##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526891_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26147 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.64244463992045 39.0 39.0 40.0 36.0 40.0 2 38.565571576089035 39.0 39.0 40.0 36.0 40.0 3 37.85589168929514 39.0 38.0 40.0 34.0 40.0 4 38.474394768042224 39.0 39.0 40.0 36.0 40.0 5 38.65789574329751 39.0 39.0 40.0 37.0 40.0 6 38.65414770336941 39.0 39.0 40.0 37.0 40.0 7 38.61999464565724 39.0 39.0 40.0 37.0 40.0 8 38.63808467510613 39.0 39.0 40.0 37.0 40.0 9 38.61024209278311 39.0 39.0 40.0 37.0 40.0 10 37.88025394882778 39.0 38.0 40.0 35.0 40.0 11 37.75148200558382 39.0 38.0 40.0 34.0 40.0 12 37.660572914674724 39.0 38.0 40.0 33.0 40.0 13 37.48525643477263 39.0 38.0 40.0 33.0 40.0 14 37.54767277316709 39.0 37.0 40.0 33.0 40.0 15 37.664053237465104 39.0 38.0 40.0 33.0 40.0 16 37.54097984472406 39.0 38.0 40.0 33.0 40.0 17 37.41297280758787 39.0 37.0 40.0 33.0 40.0 18 37.37094121696562 39.0 37.0 40.0 33.0 40.0 19 37.2025088920335 39.0 36.0 40.0 33.0 40.0 20 37.077484988717636 39.0 36.0 40.0 33.0 40.0 21 37.07396642062187 39.0 36.0 40.0 33.0 40.0 22 36.947565686312004 39.0 36.0 40.0 33.0 40.0 23 36.813974834589054 39.0 36.0 40.0 32.0 40.0 24 36.56243546104716 38.0 36.0 40.0 31.0 40.0 25 36.43213370558764 38.0 36.0 40.0 31.0 40.0 26 36.02367384403564 38.0 35.0 40.0 30.0 40.0 27 35.677515584961945 38.0 35.0 39.0 29.0 40.0 28 35.43473438635407 38.0 35.0 39.0 29.0 40.0 29 35.29376219069109 38.0 35.0 39.0 29.0 40.0 30 34.88755880215704 38.0 34.0 39.0 28.0 40.0 31 34.104371438405934 38.0 33.0 39.0 25.0 40.0 32 33.85822465292385 38.0 33.0 39.0 23.0 40.0 33 33.704019581596356 37.0 33.0 39.0 23.0 40.0 34 33.77320533904463 37.0 33.0 39.0 24.0 40.0 35 33.332160477301414 37.0 33.0 39.0 22.0 40.0 36 32.71075075534478 37.0 32.0 39.0 11.0 40.0 37 32.22549432057215 36.0 32.0 39.0 4.0 40.0 38 32.05557042872987 36.0 31.0 39.0 4.0 40.0 39 31.929437411557732 36.0 31.0 39.0 4.0 40.0 40 31.629402990782882 36.0 31.0 39.0 4.0 40.0 41 30.921482388036868 36.0 30.0 39.0 4.0 40.0 42 30.776532680613453 36.0 30.0 39.0 4.0 40.0 43 30.48039928098826 35.0 29.0 39.0 4.0 40.0 44 30.334875893984012 35.0 29.0 39.0 4.0 40.0 45 29.868474394768043 35.0 29.0 39.0 4.0 40.0 46 29.352239262630512 35.0 28.0 39.0 4.0 40.0 47 28.98168049871878 35.0 26.0 39.0 4.0 40.0 48 28.73002638926072 35.0 26.0 38.0 4.0 40.0 49 28.416835583432135 34.0 25.0 38.0 4.0 40.0 50 28.07327800512487 34.0 23.0 38.0 4.0 40.0 51 27.198034191303016 33.0 15.0 38.0 4.0 39.0 52 26.88560829158221 33.0 4.0 38.0 4.0 39.0 53 26.517879680269246 33.0 4.0 38.0 4.0 39.0 54 26.023673844035645 33.0 4.0 38.0 4.0 39.0 55 25.628408612842772 33.0 4.0 37.0 4.0 39.0 56 15.156117336596933 18.0 4.0 23.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.3594742734166658 1 2 -0.6287349647667853 1 3 0.009132762991420407 1 4 -0.3766420732534286 1 5 -0.7570395347615744 1 6 -0.3902008016584304 1 7 -0.8033607156623006 1 8 -0.71915912545429 1 9 -0.4824425162224273 1 10 -0.5228953503354958 1 11 -0.8950434806191438 1 12 -0.3977063645036125 1 13 -0.732413501481382 1 14 -0.5127597942648165 1 15 -0.18196783889518997 1 16 0.1386894328260766 1 17 0.49340068742434795 1 18 -0.345176802800637 1 19 0.265063352879352 1 20 0.21637312946332088 1 21 0.8728147893907021 1 22 0.6519026804286767 1 23 -1.1316186227565055 1 24 0.20521366502018168 1 25 -1.3234161913345588 1 26 -0.3699768207852401 1 27 -0.5061037738133862 1 28 0.3121311562459894 1 29 -0.18209616703189369 1 30 0.4341736260895672 1 31 0.5606212899221816 1 32 -1.0069851689247997 1 33 -1.9135689194853711 1 34 -0.23602308797273253 1 35 -0.7745781529453026 1 36 0.8500825829200664 1 37 0.29137437870448935 1 38 0.5037850842895111 1 39 0.09929789307462755 1 40 -0.41641250114144057 1 41 0.8383239493617758 1 42 -0.5959468093376259 1 43 0.17620647642267784 1 44 -0.42120357375765494 1 45 0.25067882214842996 1 46 0.7548853065119516 1 47 2.4685863752990755 1 48 0.9562850401662075 1 49 1.7972917483538886 1 50 0.8198862789484309 1 51 1.465764337597701 1 52 2.3966823048886994 1 53 2.0360655591305488 1 54 1.1297150985418476 1 55 2.280744778078734 1 56 0.5566255695701212 2 1 -0.19280760675000153 2 2 0.6052393942075724 2 3 0.9834917373503913 2 4 0.3413066446952939 2 5 0.4769348242127833 2 6 0.4687735573159273 2 7 -0.1751555874571764 2 8 0.43789215659698755 2 9 0.2323010735211568 2 10 -0.05494663238677333 2 11 -0.6194024549781147 2 12 -0.3848858516831015 2 13 0.19707367800580045 2 14 -0.25314440964942975 2 15 -0.5345319414592922 2 16 0.4239458430824925 2 17 -0.8495480305243746 2 18 0.7637975561737207 2 19 0.06955053236653441 2 20 0.3830397961299852 2 21 1.0266609432368554 2 22 0.020492424018421218 2 23 0.9324839413460637 2 24 1.1443162291227509 2 25 1.2118402189218571 2 26 0.3800231792147599 2 27 0.3624859697763583 2 28 1.0909773100921427 2 29 -0.3103012952370179 2 30 -0.5305699636540169 2 31 -0.9329684536675629 2 32 -0.3531390150786464 2 33 -0.4135689194853711 2 34 -1.4443564213060682 2 35 -0.37714225550940483 2 36 -1.2941481863107036 2 37 -1.5483692110390983 2 38 -1.4000610695566422 2 39 -0.6250610812843433 2 40 -0.6055150652440027 2 41 -0.5527016916638665 2 42 0.1316172932264763 2 43 0.2563346815508858 2 44 -0.2417163942704761 2 45 0.1384993349689445 2 46 0.10424428087092608 2 47 0.43974022145292224 2 48 0.28961837349953967 2 49 1.2171635432256807 2 50 1.069886278948431 2 51 1.9593540811874455 2 52 2.8133489715553672 2 53 1.8918347898997787 2 54 1.854074072900822 2 55 3.1429242652582197 2 56 -0.6997846868401361 3 1 -0.3594742734166658 3 2 0.27007455904273314 3 3 -1.4432481893895357 3 4 0.21859602198466632 3 5 -0.7153728680949101 3 6 0.2764658650082339 3 7 -0.06526547756706691 3 8 0.32250754121237435 3 9 -0.3098234686033834 3 10 -0.36813344557359073 3 11 -0.04980538538104895 3 12 -0.6834206502178972 3 13 -0.22050873957661565 3 14 0.40985925335423445 3 15 -0.33672974365709507 3 16 -1.367262948126303 3 17 -0.32207550305184185 3 18 -0.142795850419688 3 19 0.10434906716506731 3 20 0.4068493199395107 3 21 0.3013862179621327 3 22 -0.6278592243332284 3 23 -0.4649519560898341 3 24 0.6040231888297072 3 25 0.4444409515225871 3 26 0.3085946077861905 3 27 1.0474676547580373 3 28 0.2942740133888435 3 29 1.603618118682391 3 30 1.4639355308514723 3 31 1.8820498613507581 3 32 0.8025386405990105 3 33 2.13405012813368 3 34 1.5318340548844063 3 35 1.8682789899118362 3 36 1.6893682972057817 3 37 1.1366124739425842 3 38 2.4204517509561754 3 39 2.3492978930746276 3 40 1.1728732131442783 3 41 2.3740382350760605 3 42 2.4338150954242757 3 43 1.4083493335655355 3 44 1.745463092909013 3 45 3.697107393577003 3 46 1.0584567350833822 3 47 1.6888244705371704 3 48 1.2658088496900142 3 49 2.1127679388300784 3 50 1.3317910408531937 3 51 3.3348119566453214 3 52 2.5276346858410825 3 53 3.589636987701976 3 54 4.689238908065658 3 55 5.179554301888256 3 56 2.247101760046313 4 1 0.6271923932499988 4 2 0.8129317018998776 4 3 0.6957994296580878 4 4 0.5766912600799046 4 5 0.7246271319050877 4 6 0.39646586500823133 4 7 0.6433059510043648 4 8 0.7225075412123729 4 9 0.7492241504442347 4 10 0.580437982997843 4 11 0.8682898527141916 4 12 0.9089603021630523 4 13 0.35091983185195375 4 14 0.6422402057351846 4 15 1.0746988277714777 4 16 0.46702276615940974 4 17 0.2550673540910111 4 18 0.8914898638660276 4 19 0.6433966862126894 4 20 0.843039796129986 4 21 -0.12718521060929788 4 22 -0.09643065290465813 4 23 -0.5582852894231678 4 24 0.0935469983535171 4 25 -0.0450828580012228 4 26 1.3600231792147568 4 27 0.44556289285327466 4 28 1.07713115624599 4 29 1.1712371663014451 4 30 0.8125069594229046 4 31 3.123954623255514 4 32 2.913014831075202 4 33 1.9664310805146314 4 34 2.895643578693935 4 35 3.0720885137213614 4 36 2.1750825829200693 4 37 2.134707712037823 4 38 1.8737850842895085 4 39 2.16263122640796 4 40 1.8852541655252253 4 41 2.0749906160284404 4 42 2.782386523995708 4 43 3.057873143089349 4 44 1.5321297595756747 4 45 3.0323454888151 4 46 3.5365519731786215 4 47 3.3535863752990736 4 48 3.389618373499541 4 49 1.8556250816872222 4 50 2.9698862789484295 4 51 1.1024310042643677 4 52 1.8533489715553664 4 53 2.5610655591305473 4 54 1.306381765208517 4 55 -0.8909218885879326 4 56 -0.07670776376321342 5 1 -0.2761409400833372 5 2 -0.04540163143345666 5 3 -0.1158672370085796 5 4 -0.08497540658675717 5 5 0.11796046523842563 5 6 0.06813253167489819 5 7 0.2383059510043637 5 8 0.36417420787903865 5 9 0.2675574837775727 5 10 0.14377131633117557 5 11 0.6049565193808562 5 12 0.4356269688297161 5 13 0.14258649851861804 5 14 -0.22109312759815225 5 15 0.56803216110481 5 16 0.4720227661594123 5 17 0.32673402075767655 5 18 0.7381565305326916 5 19 0.3067300195460234 5 20 0.3830397961299852 5 21 0.039481456057366415 5 22 0.443569347095341 5 23 0.4100480439101659 5 24 0.12188033168685308 5 25 1.009917141998777 5 26 0.9633565125480956 5 27 1.535562892853278 5 28 1.4371311562459894 5 29 0.6095704996347777 5 30 0.5175069594229029 5 31 1.1439546232555173 5 32 1.1180148310752003 5 33 0.9614310805146289 5 34 -0.06935642130606823 5 35 1.058755180388026 5 36 2.558415916253402 5 37 1.2080410453711607 5 38 1.9204517509561754 5 39 0.8492978930746276 5 40 2.5419208321918916 5 41 2.2966572826951044 5 42 2.6540531906623706 5 43 3.00953980975601 5 44 2.7037964262423415 5 45 3.167345488815098 5 46 4.5465519731786195 5 47 4.4685863752990755 5 48 3.2896183734995397 5 49 3.6306250816872208 5 50 4.361552945615095 5 51 4.882431004264369 5 52 4.771682304888699 5 53 3.744398892463881 5 54 5.58804843187518 5 55 3.6140781114120664 5 56 2.764958902903455 6 1 0.2037441173879273 6 2 0.3094834260378079 6 3 0.4861442572442911 6 4 -0.1639983950925057 6 5 -0.3360625232673229 6 6 -0.4131893074055597 6 7 0.3288231923836733 6 8 0.08112823086754872 6 9 0.13681035734079217 6 10 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5.416913762885059 6 52 4.916797247417438 6 53 4.885203490165033 6 54 5.856726592794722 6 55 6.437353973481034 6 56 3.9646715465816165 7 1 0.21628330234090498 7 2 -0.2234319344637541 7 3 -0.0060187521600951754 7 4 0.4566912600799071 7 5 0.3755362228141834 7 6 -0.13262504408267262 7 7 0.5905786782770903 7 8 0.41341663212146784 7 9 0.21831505953514352 7 10 0.15134707390693336 7 11 0.7791989436232853 7 12 0.4053239385266849 7 13 0.305465286397407 7 14 -0.7741234306284497 7 15 0.4165170095896542 7 16 0.07429549343213893 7 17 0.5350673540910122 7 18 -0.0194192270430662 7 19 -0.09478513196913241 7 20 1.428494341584532 7 21 0.6000875166634287 7 22 0.7617511652771611 7 23 0.11080561966773672 7 24 -0.2682711834646625 7 25 0.9076444147260503 7 26 1.4709322701238534 7 27 1.967381074671458 7 28 1.8916766107914427 7 29 2.014873529937809 7 30 2.347052413968356 7 31 1.371227350528244 7 32 2.220287558347927 7 33 2.722794716878262 7 34 3.1010981241484785 7 35 3.6193612409940883 7 36 1.6114462192836996 7 37 2.420162257492372 7 38 2.117421447925878 7 39 1.72808577186251 7 40 2.307072347343407 7 41 3.75499061602844 7 42 2.089659251268433 7 43 2.672418597634799 7 44 2.8666752141211305 7 45 1.8378000342696446 7 46 2.114733791360436 7 47 4.184495466208169 7 48 0.8350729189540864 7 49 2.7556250816872208 7 50 0.8880680971302475 7 51 0.6551582769916422 7 52 1.7224398806462773 7 53 0.5701564682214588 7 54 0.4554726742994255 7 55 2.8527144750484297 7 56 2.8142013271458772 8 1 -0.21280760675000465 8 2 -0.3070682981001198 8 3 0.21579942965808385 8 4 -0.3033087399200909 8 5 0.48462713190509277 8 6 0.31646586500823304 8 7 0.32330595100436454 8 8 0.1225075412123715 8 9 0.26922415044423786 8 10 0.4204379829978393 8 11 0.9482898527141899 8 12 0.10896030216305519 8 13 1.2309198318519563 8 14 0.5622402057351863 8 15 0.914698827771474 8 16 0.4270227661594106 8 17 0.6150673540910105 8 18 0.8114898638660293 8 19 1.3633966862126883 8 20 0.6430397961299832 8 21 0.8728147893907021 8 22 0.14356934709534386 8 23 0.8817147105768299 8 24 0.2535469983535208 8 25 1.0349171419987755 8 26 1.400023179214763 8 27 1.9655628928532778 8 28 2.1971311562459874 8 29 1.4512371663014392 8 30 2.5725069594229026 8 31 2.563954623255519 8 32 3.0730148310751986 8 33 1.6864310805146303 8 34 2.9356435786939343 8 35 3.6320885137213637 8 36 3.7350825829200645 8 37 2.9747077120378265 8 38 4.15378508428951 8 39 2.5226312264079667 8 40 3.9252541655252244 8 41 3.3949906160284407 8 42 4.022386523995703 8 43 3.0978731430893482 8 44 2.4521297595756764 8 45 4.312345488815101 8 46 3.3765519731786178 8 47 1.9935863752990741 8 48 2.10961837349954 8 49 2.9756250816872196 8 50 3.1298862789484296 8 51 1.422431004264368 8 52 3.0533489715553657 8 53 3.6810655591305483 8 54 4.826381765208517 8 55 4.069078111412065 8 56 6.323292236236789 9 1 -0.37280760675000124 9 2 -0.06706829810011783 9 3 0.05579942965808726 9 4 -0.18330873992009344 9 5 -0.03537286809491036 9 6 -0.04353413499176639 9 7 0.1233059510043617 9 8 0.1225075412123715 9 9 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0.22719239325000018 117 2 0.33293170189988075 117 3 -0.14420057034191558 117 4 0.09669126007990769 117 5 0.20462713190509163 117 6 0.1964658650082356 117 7 0.04330595100436341 117 8 0.16250754121237776 117 9 -0.05077584955576242 117 10 -0.09956201700215672 117 11 -0.09171014728580928 117 12 0.22896030216305263 117 13 0.4709198318519512 117 14 0.5622402057351863 117 15 -0.5253011722285237 117 16 -0.7729772338405851 117 17 -0.2649326459089849 117 18 -0.46851013613397186 117 19 0.0033966862126888486 117 20 -0.15696020387001397 117 21 0.47281478939070354 117 22 -1.4964306529046567 117 23 -0.35828528942317206 117 24 0.45354699835351653 117 25 -1.2050828580012265 117 26 0.48002317921476134 117 27 0.9655628928532778 117 28 -0.682868843754008 117 29 1.2912371663014426 117 30 0.49250695942290434 117 31 1.2439546232555188 117 32 1.0330148310751994 117 33 1.1664310805146272 117 34 1.4956435786939295 117 35 1.1920885137213588 117 36 2.655082582920066 117 37 2.134707712037823 117 38 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119 3 -0.5198527442549619 119 4 0.28277821660164904 119 5 0.2846271319050899 119 6 0.2764658650082339 119 7 0.1893929075261056 119 8 0.4529423238210697 119 9 0.43965893305293235 119 10 -0.15695332134998807 119 11 0.05785507010549651 119 12 0.4863516065108797 119 13 0.698745918808477 119 14 0.5796315100830114 119 15 -0.20008378092417445 119 16 -0.3051511468840644 119 17 -0.20406308069159707 119 18 -0.9067710056991913 119 19 -0.23312505291774954 119 20 0.40477892656476655 119 21 0.046727832868960206 119 22 0.5957432601388177 119 23 0.24519297144639296 119 24 0.2396339548752593 119 25 0.24361279417269088 119 26 -0.46780290774176336 119 27 0.7203455015489268 119 28 0.3066963736372941 119 29 0.6947154271710048 119 30 0.9794634811620355 119 31 1.7743894058642127 119 32 1.3408409180317236 119 33 0.9994745587754963 119 34 1.2730348830417597 119 35 0.913827644156143 119 36 3.105517365528762 119 37 2.5051424946465204 119 38 3.536393779941683 119 39 1.9869790524949167 119 40 3.16873242639479 119 41 0.36368626820235406 119 42 1.514560437039183 119 43 1.5222209691762991 119 44 1.4121297595756772 119 45 2.4879976627281444 119 46 2.6878563210047055 119 47 2.8109776796469035 119 48 3.6157053300212816 119 49 4.081712038208963 119 50 4.178581931122345 119 51 2.512865786873064 119 52 2.9003054932944963 119 53 2.856717733043592 119 54 2.720294808686777 119 55 3.9890781114120664 119 56 1.679813975367221 120 1 0.07991966597727185 120 2 -0.9961592071910275 120 3 -0.5969278430691887 120 4 0.138509441898087 120 5 -0.30628195900400357 120 6 -0.2235341349917661 120 7 -0.4094213217229097 120 8 -0.26840154969671914 120 9 -0.054412213192129855 120 10 -0.7577438351839803 120 11 0.4155625799869185 120 12 0.04168757489032515 120 13 0.7600107409428603 120 14 0.5440583875533633 120 15 0.4165170095896542 120 16 0.4379318570685058 120 17 0.35324917227283237 120 18 -0.06487377249760584 120 19 0.9506694134854143 120 20 0.8830397961299852 120 21 1.554632971208882 120 22 1.0344784380044345 120 23 0.6108056196677367 120 24 0.7771833619898842 120 25 0.27128077836241005 120 26 1.8345686337602132 120 27 1.467381074671458 120 28 1.2553129744278095 120 29 2.287600802665075 120 30 2.6652342321501763 120 31 2.325772805073697 120 32 2.4930148310752003 120 33 1.8591583532419023 120 34 2.510189033239385 120 35 2.846633968266815 120 36 2.6569007647382463 120 37 3.238344075674192 120 38 3.1628759933804176 120 39 3.54626759004433 120 40 3.4434359837070474 120 41 0.8004451614829868 120 42 2.1805683421775264 120 43 2.7633276885438924 120 44 0.6848570323029506 120 45 2.746890943360551 120 46 3.387461064087713 120 47 3.002677284389982 120 48 3.60780019168136 120 49 0.5283523544144941 120 50 1.7062499153120676 120 51 1.836976458809822 120 52 0.9497126079190039 120 53 1.7065201045850955 120 54 3.228199947026699 120 55 3.216350838684793 120 56 3.2232922362367873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 15.0 5 4.0 6 2.0 7 4.0 8 11.0 9 12.0 10 15.0 11 30.0 12 25.0 13 58.0 14 72.0 15 76.0 16 128.0 17 156.0 18 185.0 19 213.0 20 243.0 21 299.0 22 304.0 23 339.0 24 477.0 25 691.0 26 572.0 27 546.0 28 576.0 29 574.0 30 672.0 31 778.0 32 907.0 33 1154.0 34 1538.0 35 2017.0 36 2768.0 37 3900.0 38 4453.0 39 2333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.25218954373351 1.0211496538799862 0.15680575209393047 0.5698550502925767 8 98.84477086680438 0.7765281921811644 0.16448626730931068 0.21421467370514882 9 97.0551114850652 1.3959536466898688 0.9790798179523463 0.5698550502925767 10 54.33510536581635 22.350556469193407 15.007457834550808 8.306880330439439 11 39.38884001988756 27.735495467931308 15.003633304011931 17.872031208169197 12 36.15711171453704 13.416453130378244 27.096798867938958 23.329636287145753 13 28.569243125406356 17.034459020155275 24.905342869162812 29.490954985275557 14 22.664167973381268 20.7824989482541 34.31751252533751 22.235820553027118 15 25.807932076337632 20.88958580334264 29.471832332581176 23.830649787738555 16 29.349447355337134 18.755497762649632 26.46957585956324 25.42547902244999 17 31.83539220560676 19.271809385397944 24.473170918269783 24.419627490725514 18 32.70738516847057 19.13795081653727 24.010402723065745 24.144261291926416 19 31.173748422381152 19.51275480934715 24.186331127854054 25.127165640417637 20 26.767889241595594 26.285998393697174 22.09431292308869 24.851799441618542 21 27.651355796076032 19.21826595785367 29.999617546946112 23.130760699124185 22 28.473629861934448 18.682831682410985 24.54966152904731 28.293876926607258 23 26.83673079129537 24.905342869162812 24.12131410869316 24.136612230848662 24 25.371935594905725 27.52514628829311 23.85742150151069 23.245496615290474 25 29.231534253911178 23.83812110316337 20.120108633286158 26.810236009639294 26 27.70337491390526 19.759700007652867 22.445855973061914 30.091069105379965 27 22.658043777743764 18.647635083422625 26.484769631103628 32.20955150772999 28 25.968606431852987 25.662327718223583 22.90199081163859 25.467075038284836 29 30.650166660281215 23.474196390942875 21.627523849660932 24.248113099114978 30 26.72060120394157 22.30742686246693 24.680802116483264 26.29116981710824 31 28.01795235720588 22.766504277110744 24.60009973531781 24.61544363036557 32 31.255514212282787 22.206452107867584 22.904599332540563 23.633434347309066 33 32.113259668508285 19.774401473296503 23.98327194597913 24.129066912216082 34 27.765626798664673 23.966847012777713 27.151682590844555 21.11584359771306 35 24.3752639054163 25.945261218379333 28.81271352347319 20.86676135273118 36 28.79358009522347 26.025188143142376 23.96329288895715 21.21793887267701 37 27.407834101382488 24.592933947772657 26.658986175115206 21.340245775729645 38 30.56441387789603 23.8175740577093 24.674376608906137 20.943635455488533 39 26.137761377613778 22.378536285362856 24.880842558425584 26.602859778597786 40 27.26293517984228 23.154452779380648 25.91267551452202 23.66993652625505 41 24.216765453005927 21.599568932337775 29.909167885459166 24.274497729197137 42 24.861431870669744 21.000769822940725 27.7328714395689 26.40492686682063 43 22.650955021565004 21.557301293900185 32.34750462107209 23.444239063462724 44 26.05213507373609 19.21758884910092 29.398174887374378 25.33210118978861 45 23.82328017872275 20.56081965950235 28.584084431091593 27.031815730683306 46 23.625081897714573 20.457085597564266 27.301807530735733 28.616024973985432 47 25.035660588303326 22.16353753035969 28.428235475538767 24.372566405798217 48 24.510106465051688 25.852491899398238 26.519827187162477 23.117574448387597 49 28.158747300215982 22.697469916692377 26.02591792656587 23.117864856525763 50 24.88422352577956 22.40660697746218 26.945044766903365 25.764124729854892 51 23.113425925925927 27.619598765432094 24.872685185185187 24.39429012345679 52 24.276452882611714 25.70039360963186 27.274832137068767 22.74832137068766 53 25.4669651126891 23.139857980858288 28.51574560049398 22.87743130595863 54 23.577678397822282 23.597122302158276 26.863698230604705 25.961501069414737 55 27.169170174151446 23.10692358188207 26.867976985751245 22.855929258215237 56 23.322141175562503 23.534406236733435 26.32086758519548 26.822585002508585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 3.5 9 5.0 10 5.5 11 6.0 12 11.0 13 22.0 14 28.0 15 29.5 16 31.0 17 45.5 18 60.0 19 81.0 20 102.0 21 105.0 22 104.5 23 101.0 24 133.5 25 166.0 26 168.0 27 170.0 28 268.5 29 425.0 30 483.0 31 446.5 32 410.0 33 447.5 34 485.0 35 489.0 36 493.0 37 526.5 38 446.0 39 332.0 40 367.0 41 402.0 42 376.0 43 350.0 44 422.0 45 494.0 46 583.5 47 849.5 48 1026.0 49 1059.5 50 1093.0 51 954.0 52 815.0 53 885.0 54 990.0 55 1025.0 56 1157.5 57 1290.0 58 1316.5 59 1343.0 60 1569.5 61 1796.0 62 1998.5 63 2247.0 64 2293.0 65 2009.5 66 1726.0 67 1539.5 68 1353.0 69 1214.5 70 1076.0 71 936.5 72 665.0 73 533.0 74 436.0 75 339.0 76 313.5 77 288.0 78 217.5 79 123.5 80 100.0 81 75.0 82 50.0 83 38.5 84 27.0 85 20.0 86 13.0 87 12.0 88 7.0 89 3.0 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.019122652694381764 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.015298122155505411 26 0.04971889700539258 27 0.05736795808314529 28 0.10326232454966153 29 0.17592840478831223 30 0.2524190155658393 31 0.2983133820323555 32 0.2983133820323555 33 0.3174360347267373 34 0.32890962634336635 35 0.3671549317321299 36 0.39392664550426437 37 0.40922476765976973 38 0.45894366466516234 39 0.5010135005928023 40 0.5813286419092056 41 0.6310475389145982 42 0.638696599992351 43 0.6845909664588672 44 0.6731173748422381 45 0.7075381496921253 46 0.7649061077752706 47 0.7955023520862813 48 0.8528703101694266 49 0.8375721880139212 50 0.898764676635943 51 0.8681684323249321 52 0.8911156155581902 53 0.898764676635943 54 1.6521971927945844 55 0.9561326347190882 56 0.9025892071748193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 26147.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.87547328565419 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00974596580923 33.9962519600719 2 13.460616711934815 12.888667916013311 3 5.6398785748522124 8.100355681340115 4 3.163444639718805 6.058056373580143 5 1.7335037545933856 4.149615634680843 6 1.1104010225275602 3.1896584694228785 7 0.7908611599297013 2.6503996634413123 8 0.5591947595462534 2.1417371017707576 9 0.4393673110720563 1.8931426167437946 >10 1.9491931618469405 16.483726622557082 >50 0.09586195877935773 3.109343328106475 >100 0.047930979389678864 5.3390446322713885 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 321 1.2276743029793094 No Hit ACACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 269 1.028798714957739 No Hit ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 264 1.0096760622633572 No Hit ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 227 0.8681684323249321 No Hit ACACCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 204 0.780204229930776 No Hit ACACCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 111 0.4245228898152752 No Hit ACACCCGGGGGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCT 93 0.35568134011550084 No Hit ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 87 0.3327341568822427 No Hit ACACCCGGGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTATAGTA 80 0.30596244311010823 No Hit ACACCCGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 77 0.2944888514934792 No Hit ACACCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 67 0.25624354610471567 No Hit ACACCCGGGCATGTGTTTCAAAATAAGGGAACTTCAGTCACCTCAAAGGTAAAATA 67 0.25624354610471567 No Hit ACACCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 61 0.23329636287145752 No Hit ACACCCGGGGAACTACTTAGTCAAATGTGATACCATAAATTTAAACAGTTTACCAT 60 0.22947183233258117 No Hit ACACCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 60 0.22947183233258117 No Hit ACACCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 55 0.2103491796381994 No Hit ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 54 0.20652464909932305 No Hit ACACCCGGGGTGTAACCTTTAGAATTATTTGCAGAATATCCTTTTAGCTTATATGT 52 0.19887558802157032 No Hit ACACCCGGGACTGCTTAGATGGTGGGCCTGCCCTCACCAAACAGGAATTTCTATTA 50 0.19122652694381764 No Hit ACACCCGGGGTGGACCTTCCTTCCTCCCATGGCATGTGCAAGTAGGTCTGCGTGAC 49 0.1874019964049413 No Hit ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 47 0.17975293532718858 No Hit ACACCCGGGTTGCCCCATCCCCAAACCTGTCCAGCACTTATGTAAGAACGCATTTG 47 0.17975293532718858 No Hit ACACCCGGGCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTGATTGTGATG 47 0.17975293532718858 No Hit ACACCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 46 0.17592840478831223 No Hit ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.17210387424943588 No Hit ACACCCGGGGGTGCCAGAGAGAGTAGAGGAGAGCAGAGAGCCGTGGAGCGACAGTG 44 0.16827934371055953 No Hit ACACCCGGGGGTTTCCACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCC 42 0.16063028263280682 No Hit ACACCCGGGGGCTATTACCTCATTCCTTTCACTGGGATTGTAGGATTACTGGTTTT 42 0.16063028263280682 No Hit ACACCCGGGATGTGCCCCGGGCAGTGTTCGTAGACCTGGAACCCACGGTCATCGAT 41 0.15680575209393047 No Hit ACACCCGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 41 0.15680575209393047 No Hit ACACCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC 40 0.15298122155505411 No Hit ACACCCGGGGGTGCTGGAGCCACTGTCGCCGAGCTCGGGCCACGCTGCTTCTCCTC 37 0.14150762993842506 No Hit ACACCCGGGGAGCCGCTCCTGCGCGCTCCCGGGCTTGGAGTCCGAGCACGTTCTCG 37 0.14150762993842506 No Hit ACACCCGGGGCTCTAAAATGGAAGTGGAGCGGACTCCTAGTACCGCTAGAAGCTGC 36 0.1376830993995487 No Hit ACACCCGGGGATTGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTT 32 0.12238497724404329 No Hit ACACCCGGGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA 32 0.12238497724404329 No Hit ACACCCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 32 0.12238497724404329 No Hit ACACCCCGTCATTTTGGCCTCGATCGTGGACAGCTACGAACGCCGCAACGAGGGAG 31 0.11856044670516692 No Hit ACACCCGGGAGCATTGCAGAGACACCAACTCCAGTAATTGTAGTCACCTCTTCCTT 31 0.11856044670516692 No Hit ACACCCGGGCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAG 30 0.11473591616629059 No Hit ACACCCGGGCTTGTGTCAGATGACATCATGGACTCTTCCCTCACTCGCCGGGGACA 30 0.11473591616629059 No Hit ACACCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCG 30 0.11473591616629059 No Hit ACACCCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT 29 0.11091138562741425 No Hit ACACCCAGGGGACACCGTCGTAGAGTCGTCTGGGTTGAACCGCTGGGCCGAAAGCT 29 0.11091138562741425 No Hit ACACCCGGGGACGAGATGGGGGAAAGCATGGCAGATGATCCACGGAATTTAAAAAA 28 0.10708685508853788 No Hit ACACCCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGC 28 0.10708685508853788 No Hit ACACCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA 28 0.10708685508853788 No Hit ACACCCGGGGTGGCTTACCTCGTTCACCAAATTGGGTGCACACTCACTGCTCTTGG 28 0.10708685508853788 No Hit ACACCCGGGAACACATGGCAGTTCAACTTCAAGTTCAACCTGACAGAGGATATGTA 27 0.10326232454966153 No Hit ACACCCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 27 0.10326232454966153 No Hit ACACCCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 27 0.10326232454966153 No Hit ACACCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.10326232454966153 No Hit ACACCCGGGGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGACCT 27 0.10326232454966153 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.003824530538876353 0.0 0.0 0.0 0.0 9 0.003824530538876353 0.0 0.0 0.0 0.0 10 0.003824530538876353 0.0 0.0 0.0 0.0 11 0.003824530538876353 0.0 0.0 0.0 0.0 12 0.02677171377213447 0.0 0.0 0.0 0.0 13 0.08796420239415612 0.0 0.0 0.0 0.0 14 0.10708685508853788 0.0 0.0 0.0 0.0 15 0.11091138562741423 0.0 0.0 0.0 0.0 16 0.11091138562741423 0.0 0.0 0.0 0.0 17 0.11091138562741423 0.0 0.0 0.0 0.0 18 0.11856044670516694 0.0 0.0 0.0 0.0 19 0.11856044670516694 0.0 0.0 0.0 0.0 20 0.130034038321796 0.0 0.0 0.0 0.0 21 0.1376830993995487 0.0 0.0 0.0 0.0 22 0.14150762993842506 0.0 0.0 0.0 0.0 23 0.15298122155505411 0.0 0.0 0.0 0.0 24 0.16827934371055953 0.0 0.0 0.0 0.0 25 0.16827934371055953 0.0 0.0 0.0 0.0 26 0.16827934371055953 0.0 0.0 0.0 0.0 27 0.16827934371055953 0.0 0.0 0.0 0.0 28 0.17975293532718858 0.0 0.0 0.0 0.0 29 0.17975293532718858 0.0 0.0 0.0 0.0 30 0.1874019964049413 0.0 0.0 0.0 0.0 31 0.19122652694381764 0.0 0.0 0.0 0.0 32 0.2027001185604467 0.0 0.0 0.0 0.0 33 0.2027001185604467 0.0 0.0 0.0 0.0 34 0.2027001185604467 0.0 0.0 0.0 0.0 35 0.22947183233258117 0.0 0.0 0.0 0.0 36 0.22947183233258117 0.0 0.0 0.0 0.0 37 0.23329636287145752 0.0 0.0 0.0 0.0 38 0.23712089341033388 0.0 0.0 0.0 0.0 39 0.23712089341033388 0.0 0.0 0.0 0.0 40 0.24094542394921023 0.0 0.0 0.0 0.0 41 0.24859448502696294 0.0 0.0 0.0 0.0 42 0.24859448502696294 0.0 0.0 0.0 0.0 43 0.2524190155658393 0.0 0.0 0.0 0.0 44 0.25624354610471567 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTGA 15 0.0078337705 50.79492 49 GCGTGAC 15 0.0078337705 50.79492 50 ATTGCTT 20 3.836463E-4 50.695908 48 CAGCAAG 30 9.670639E-7 50.303677 47 CCAGCAA 30 9.670639E-7 50.303677 46 CTCACTG 15 0.008141466 50.303677 46 TCTGCGT 20 3.9869474E-4 50.303673 47 TTACCTC 15 0.008204068 50.206566 42 AGTAGGT 15 0.008204068 50.206566 41 TGCCCAG 35 4.8647053E-8 50.206566 43 CTTACCT 15 0.008204068 50.206566 41 ACCTCAC 15 0.008204068 50.206566 44 GTAGGTC 15 0.008204068 50.206566 42 TACCTCA 15 0.008204068 50.206566 43 TAGGTCT 15 0.008204068 50.206566 43 ACAGCAT 20 4.0252914E-4 50.206562 43 TGACAGC 20 4.0252914E-4 50.206562 41 AGCATTG 20 4.0252914E-4 50.206562 45 AAGTAGG 15 0.008267027 50.10983 40 TGCACCG 15 0.008267027 50.10983 38 >>END_MODULE