FastQCFastQC Report
Fri 17 Jun 2016
SRR1526890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54295
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4000.7367160880375725No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.5046505203057372No Hit
CGGCAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.3075789667556865No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.29837001565521687No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.20812229487061426No Hit
CGGCAGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGGGAAGACAAGCAAA680.12524173496638732No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.1233999447462934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGA150.004501906758.51724256
ACATCTA150.00465347958.03049540
CAAAAAG150.00465347958.03049543
CATTTTA201.9971607E-457.9769232
CGGGTAA201.9971607E-457.9769230
TGGGTTA201.9971607E-457.9769230
ACTCTAC150.004670551557.97691336
CATTTCC150.004670551557.97691336
GGTTAGA150.004670551557.97691332
TACCCCG150.004670551557.97691325
TTAACTC150.004670551557.97691336
ACGGCTT150.004670551557.97691324
AAAGACA150.004670551557.97691327
TTTAACT150.004670551557.97691335
ATCTCAT202.0063091E-457.9234312
TCGGTTC150.00468767157.92342815
ATCGGGG150.00468767157.92342811
TAACAAA150.00468767157.92342822
TCATCGC150.00468767157.92342822
CTGTCAT150.00468767157.92342814
Produced by FastQC (version 0.11.5)