FastQCFastQC Report
Fri 17 Jun 2016
SRR1526889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78927
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10121.2821974736148594No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.6208268399913844No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3810.48272454293207645No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2570.3256173426077261No Hit
CGGCAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.3142144006487007No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1770.2242578585275001No Hit
CGGCAGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGGGAAGACAAGCAAA1750.22172387142549446No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1740.22045687787449164No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.21792289077248597No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1720.21792289077248597No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.2090539359154662No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1650.2090539359154662No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1560.19765099395644076No Hit
CGGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1540.19511700685443512No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1490.18878203909942098No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1430.18118007779340403No Hit
CGGCAGGGGATCTTCCTGACCTATTGTAGAACACAGGGGCACTGCATTCTGGGAATCAACCTAC1430.18118007779340403No Hit
CGGCAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1410.17864609069139836No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1380.17484511003838993No Hit
CGGCAGGGGACTGGCTCCAAGCTCCCTACCTGTATAAACTCTATCAGCATTACCACTTCCTGGA1270.16090818097735882No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG1250.15837419387535318No Hit
CGGCAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1120.14190327771231645No Hit
CGGCAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1110.1406362841613136No Hit
CGGCAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1100.13936929061031078No Hit
CGGCAGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG970.12289837444727407No Hit
CGGCAGGGGGAAAGCCTTTGTCATCACCCTACTCCCTCCCCAACCCAGACTCCAGACCCTGTTT950.12036438734526841No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.12036438734526841No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG910.11529641314125712No Hit
CGGCAGGGGTCACCTCCTCCCTACATCAGCTCTGGTCTTTCTTCCTCTTTATCCACCCCTGCTG900.1140294195902543No Hit
CGGCAGGGGGGTCCCTCGCGCAGGAATGGGCCCGCAGGCGGCAGCTGGCAGGATGATTCTGCTG900.1140294195902543No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT860.10896144538624299No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC850.10769445183524017No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC840.10642745828423733No Hit
CGGCAGGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATGGGTCAAATTGTGTCTTCCA830.10516046473323451No Hit
CGGCAGGGGGTTTTTACCTGCGCCGTAACGCAGCCGGTTTACACGTGACTTGCATAGTGTGTAC800.10135948408022603No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG790.1000924905292232No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT790.1000924905292232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG150.004621431758.1565958
AATCAAC150.004621431758.1565954
ATCTAAG150.004621431758.1565956
TTGTGCA150.004621431758.1565951
ACTTATT150.004621431758.1565955
GACCACT150.004621431758.1565951
CTTATTC150.004621431758.1565956
ACAGGAT150.004621431758.1565953
CCACGCT150.004621431758.1565958
TTATTCG150.004621431758.1565957
CACTTAT150.004621431758.1565954
CGGCCCA201.9785707E-458.11959547
AGCATTA201.9785707E-458.11959546
GGACCAC150.004633099358.11959550
ACTCCAG150.004633099358.11959549
CCACATG150.004633099358.11959547
CAGCATT201.9910601E-458.0457445
TGATCAA150.00466823558.0088929
TATCAGC201.9973284E-458.0088942
CAGCGAT150.00466823558.0088933
Produced by FastQC (version 0.11.5)