FastQCFastQC Report
Fri 17 Jun 2016
SRR1526888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79015
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9771.236474087198633No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.5606530405619186No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3890.4923115864076441No Hit
CGGCAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.37461241536417134No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2010.2543820793520218No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.21894576979054609No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1650.20882110991583877No Hit
CGGCAGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGGGAAGACAAGCAAA1570.19869645004113143No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1520.19236853761943934No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.18730620768208567No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1440.182243877744732No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1430.1809782952603936No Hit
CGGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1390.17591596532303994No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1380.17465038283870152No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG1370.1733848003543631No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1350.17085363538568626No Hit
CGGCAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1340.16958805290134785No Hit
CGGCAGGGGATCTTCCTGACCTATTGTAGAACACAGGGGCACTGCATTCTGGGAATCAACCTAC1270.16072897551097892No Hit
CGGCAGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1210.15313548060494842No Hit
CGGCAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1200.15186989812061No Hit
CGGCAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1170.14807315066759477No Hit
CGGCAGGGGACTGGCTCCAAGCTCCCTACCTGTATAAACTCTATCAGCATTACCACTTCCTGGA1110.14047965576156424No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1100.13921407327722585No Hit
CGGCAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1010.1278238309181801No Hit
CGGCAGGGGGAAAGCCTTTGTCATCACCCTACTCCCTCCCCAACCCAGACTCCAGACCCTGTTT970.12276150098082643No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.11263684110611909No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC890.11263684110611909No Hit
CGGCAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT870.11010567613744225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAAGG150.0045287758.45448745
TTTGTAT150.004540285658.4170439
GCAGTAG150.004540285658.4170440
TAAGTTC150.004540285658.4170439
TCCAGTA150.004540285658.4170442
ACCCCAA150.004540285658.4170441
GTGCGAA150.004540285658.4170443
CATGTCC201.9291851E-458.41703843
TAGCGCT150.004551822358.37964226
CTAAGAA150.004551822358.37964225
CCGTCGG150.004551822358.37964224
GTCCGAC150.004551822358.37964225
CGTCGGT150.004551822358.37964225
TGCTAGC150.004551822358.37964223
TAAGAAA150.004551822358.37964226
AAACTAA150.004563381458.34229322
CATCACC150.004563381458.34229322
CAAACTA150.00457496258.30498521
GGCTTCG150.00457496258.30498521
ATATTCT150.00457496258.30498521
Produced by FastQC (version 0.11.5)