FastQCFastQC Report
Fri 17 Jun 2016
SRR1526887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71222
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG10401.4602229648142429No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4680.6571003341664092No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3430.48159276628008196No Hit
CGGCAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.4380668894442728No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2580.36224762011737943No Hit
CGGCAGGGGATCTTCCTGACCTATTGTAGAACACAGGGGCACTGCATTCTGGGAAT2070.29064053241975796No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1870.26255932155794554No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.2190334447221364No Hit
CGGCAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC1500.2106090814635927No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1440.202184718205049No Hit
CGGCAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1430.20078065766195838No Hit
CGGCAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1420.1993765971188678No Hit
CGGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1340.18814411277414284No Hit
CGGCAGGGGGAGTCTGAAACCATCTGAGCAGAGTCTCTCTTCAGTCCCCGGGAAGA1290.18112381005868972No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG1230.172699446800146No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1190.16708320462778353No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.1642750835416023No Hit
CGGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1160.1628710229985117No Hit
CGGCAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1150.16146696245542108No Hit
CGGCAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1140.16006290191233047No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1070.15023447811069612No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG970.13619387267978994No Hit
CGGCAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC960.13478981213669936No Hit
CGGCAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG940.1319816910505181No Hit
CGGCAGGGGACTGGCTCCAAGCTCCCTACCTGTATAAACTCTATCAGCATTACCAC880.12355732779197438No Hit
CGGCAGGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT880.12355732779197438No Hit
CGGCAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC880.12355732779197438No Hit
CGGCAGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC870.12215326724888376No Hit
CGGCAGGGGTCACCTCCTCCCTACATCAGCTCTGGTCTTTCTTCCTCTTTATCCAC840.1179410856196119No Hit
CGGCAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.1179410856196119No Hit
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA810.11372890399034005No Hit
CGGCAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG800.11232484344724945No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC760.10670860127488697No Hit
CGGCAGGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC760.10670860127488697No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC760.10670860127488697No Hit
CGGCAGGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATGGGTCAAATTGT740.10390048018870575No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC740.10390048018870575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATC150.00791767750.75071749
GAACGGT150.00791767750.75071749
AGTATCC150.00791767750.75071750
AAAAGGA150.00791767750.75071748
ATTAAGC251.8927614E-550.75071750
TACCCGG203.8620416E-450.75071750
AACGGTA150.00791767750.75071750
TTGTGCA150.00791767750.75071750
GGTACCC150.00791767750.75071748
GCGCCCC204.043044E-450.2824745
CAGCATT150.00821346650.28246745
CGCCCCT150.00821346650.28246746
AATTACT150.00821346650.28246742
ATTACTC150.00821346650.28246743
TCAGCAT150.00821346650.28246744
TCTCGAT150.00821346650.28246743
TATCAGC150.00821346650.28246742
AGCATTA150.00821346650.28246746
ATTCTCG150.00821346650.28246741
CGATCCA150.00821346650.28246746