FastQCFastQC Report
Fri 17 Jun 2016
SRR1526886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29881
Sequences flagged as poor quality0
Sequence length64
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.9604765570094709No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.7563334560423012No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.6994411164284997No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.6090826946889328No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.4283658512097989No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.4250192429972223No Hit
ACTTATGGGATTTTTAAAAAATGTGTTTATATTTTACTTGAAGACACTATGTAGCATGAATTGG960.3212743884073492No Hit
ACTTATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.2543422241558181No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.22087614203005254No Hit
ACTTATGGGAACGATGTTGAACTTGTTGCTGAGGGCAACAGCAGGTTCACCTACTCTGTCCTAG610.20414310096716978No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.19744988454201665No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.1941032763294401No Hit
ACTTATGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.18071684347913392No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.17737023526655735No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.1606371942036746No Hit
ACTTATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.15729058599109802No Hit
ACTTATGGGGGCCATGTGTATGCAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTG450.1505973695659449No Hit
ACTTATGGGGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAA390.13051772029048558No Hit
ACTTATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.11043807101502627No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10709146280244973No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG310.10374485458987316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCA1700.058.1163645
ATGGGTT303.5873927E-758.116365
TTATGGC303.5873927E-758.116363
TGGGGTT351.5446858E-858.116366
TGGGGGA900.058.116366
TGGGGCT700.058.116366
GGGACTT150.00460572558.116367
GGGATTC201.9589523E-458.116367
TGGGCTA201.9589523E-458.116366
TGGGTGC201.9589523E-458.116366
TGGGCAT452.910383E-1158.116366
TGGGCAA150.00460572558.116366
TGGGAGA800.058.116366
GGGCCTA150.00460572558.116367
TATGGCA150.00460572558.116364
TATGGAG303.5873927E-758.116364
ATGGGCT1000.058.1163565
ATGGGAC2150.058.1163565
TGGGGTC550.058.1163566
GGGATTA258.3640625E-658.1163567
Produced by FastQC (version 0.11.5)