FastQCFastQC Report
Fri 17 Jun 2016
SRR1526885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35956
Sequences flagged as poor quality0
Sequence length64
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4621.284903771276004No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.873289576148626No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.7954166203137167No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.5367671598620536No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.5339859828679497No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.44776949605072863No Hit
ACTTATGGGATTTTTAAAAAATGTGTTTATATTTTACTTGAAGACACTATGTAGCATGAATTGG1520.42273890310379353No Hit
ACTTATGGGGGCCATGTGTATGCAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTG1270.3532094782511959No Hit
ACTTATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.26699299143397487No Hit
ACTTATGGGAACGATGTTGAACTTGTTGCTGAGGGCAACAGCAGGTTCACCTACTCTGTCCTAG860.23918122149293583No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.23083769051062408No Hit
ACTTATGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.21136945155189676No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG750.20858827455779286No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.20580709756368892No Hit
ACTTATGGGATCCAAACGTTTCTACAGTCACCCAGAGACTAATGAAGGCATACACATTTTTTTT730.20302592056958504No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.19746356658137723No Hit
ACTTATGGGGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAA640.1779953276226499No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.1779953276226499No Hit
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1779953276226499No Hit
ACTTATGGGAAGGCTGAAGAGGTGCCCTCTCCGGCCCACTTCTGTTCTCCTCAAGCCATCCCAG630.175214150628546No Hit
ACTTATGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACG630.175214150628546No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1724329736344421No Hit
ACTTATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.15852708866392257No Hit
ACTTATGGGGCAGACATTTATACTCTGCATTTTGAAGCCAGTAATTTGCAAGATACCAAAGAAA540.15018355768161085No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG500.13905884970519525No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.13349649571698743No Hit
ACTTATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG450.12515296473467571No Hit
ACTTATGGGAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGATGAGGAGACGGG450.12515296473467571No Hit
ACTTATGGGGTTGCTGCCGGGCAGTGTGTGGGGACTGGGGCTGGCAGCATTTGCCAGGGAAAGC430.11959061074646792No Hit
ACTTATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.1140282567582601No Hit
ACTTATGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1112470797641562No Hit
ACTTATGGGTCGAGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.10568472577594838No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG380.10568472577594838No Hit
ACTTATGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10290354878184446No Hit
ACTTATGGGATTATAGAGATTGGCAACAGCAGCTCTCAAACCCTTGATACCTGCTGTAGGACCA370.10290354878184446No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT360.10012237178774056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAT150.00462836558.0696455
GCAGCCC150.00462836558.0696453
GCATGAA150.00462836558.0696454
GGAGATG150.004653990357.9888768
GGATGTC150.004653990357.9888768
GAGGTCC150.004653990357.98887624
TGTTGAA150.004653990357.98887615
CAAGAGT150.004653990357.98887615
GTGTCTT150.004653990357.98887640
GTGTTTA150.004653990357.98887623
GTTGAAC150.004653990357.98887616
GGCATGC150.004653990357.9888768
TATATTT150.004653990357.98887628
TCAAGAG150.004653990357.98887614
GAGTGTA150.004653990357.98887611
CGCCTTG150.004653990357.98887617
TGTCTTG150.004653990357.98887641
TGACTGC150.004653990357.98887643
TATGTAG150.004653990357.98887648
GAAGTGG150.004653990357.9888769
Produced by FastQC (version 0.11.5)