FastQCFastQC Report
Fri 17 Jun 2016
SRR1526884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35444
Sequences flagged as poor quality0
Sequence length64
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3701.0439002369935673No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.7533009818304932No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.7363728698792461No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.5191287665049091No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.5022006545536621No Hit
ACTTATGGGATTTTTAAAAAATGTGTTTATATTTTACTTGAAGACACTATGTAGCATGAATTGG1580.445773614716172No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.3695971109355603No Hit
ACTTATGGGGGCCATGTGTATGCAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTG1220.34420494300868976No Hit
ACTTATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.25674303126058007No Hit
ACTTATGGGAACGATGTTGAACTTGTTGCTGAGGGCAACAGCAGGTTCACCTACTCTGTCCTAG890.25110032727683107No Hit
ACTTATGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.21160139939058797No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG660.1862092314637174No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.1805665274799684No Hit
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1777451754880939No Hit
ACTTATGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACG620.1749238234962194No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.17210247150434488No Hit
ACTTATGGGAAGGCTGAAGAGGTGCCCTCTCCGGCCCACTTCTGTTCTCCTCAAGCCATCCCAG610.17210247150434488No Hit
ACTTATGGGATCCAAACGTTTCTACAGTCACCCAGAGACTAATGAAGGCATACACATTTTTTTT610.17210247150434488No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.16645976752059588No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.16081706353684685No Hit
ACTTATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.15517435955309786No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.15235300756122333No Hit
ACTTATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1438889515855998No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.1438889515855998No Hit
ACTTATGGGGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAA500.1410675995937253No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG440.12413948764247829No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG430.12131813565060376No Hit
ACTTATGGGAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGATGAGGAGACGGG400.11285407967498026No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA390.11003272768310575No Hit
ACTTATGGGGCAGACATTTATACTCTGCATTTTGAAGCCAGTAATTTGCAAGATACCAAAGAAA380.10721137569123124No Hit
ACTTATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG370.10439002369935672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCA150.004484247458.53219245
AAGGCAT150.004484247458.53219245
GAAGGCA150.004484247458.53219244
GACTCCT150.00450975558.44857439
TGAGAGA150.00450975558.44857439
CCATGAT201.9365201E-458.28205526
CTACAGT150.004561093658.2820522
ACAGTCA150.004561093658.2820524
TGCGCGC150.004561093658.2820525
CCTGAGC303.5364428E-758.2820520
GAGCCAT258.255707E-658.2820523
CCGTCCA150.004561093658.2820526
CGGATGA150.004561093658.2820525
TCTACAG150.004561093658.2820521
GAGTCCC150.004561093658.2820523
GTCCCTT150.004561093658.2820525
CACTCCG150.004561093658.2820522
TTCTACA150.004561093658.2820520
CGTAAAC150.004561093658.2820521
TCCGTCC150.004561093658.2820525
Produced by FastQC (version 0.11.5)