SRR1526883_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526883_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29470
Sequences flagged as poor quality	0
Sequence length	56
%GC	35

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	449	1.5235833050559893	No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	237	0.8042076688157448	No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	218	0.7397353240583645	No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	188	0.6379368849677638	No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	187	0.6345436036647438	No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	134	0.4546996946046827	No Hit
ACTTATGGGATTTTTAAAAAATGTGTTTATATTTTACTTGAAGACACTATGTAGCA	117	0.3970139124533424	No Hit
ACTTATGGGGGCCATGTGTATGCAGTATTCGAGACAGCTACAAGGAACACAGGCAA	97	0.32914828639294197	No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76	0.2578893790295216	No Hit
ACTTATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.2443162538174415	No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.2341364099083814	No Hit
ACTTATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	69	0.2341364099083814	No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	69	0.2341364099083814	No Hit
ACTTATGGGATCCAAACGTTTCTACAGTCACCCAGAGACTAATGAAGGCATACACA	64	0.2171700033932813	No Hit
ACTTATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	58	0.19681031557516118	No Hit
ACTTATGGGAACGATGTTGAACTTGTTGCTGAGGGCAACAGCAGGTTCACCTACTC	58	0.19681031557516118	No Hit
ACTTATGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.17984390906006106	No Hit
ACTTATGGGAAGGCTGAAGAGGTGCCCTCTCCGGCCCACTTCTGTTCTCCTCAAGC	53	0.17984390906006106	No Hit
ACTTATGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC	52	0.17645062775704107	No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52	0.17645062775704107	No Hit
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52	0.17645062775704107	No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	52	0.17645062775704107	No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.17305734645402104	No Hit
ACTTATGGGGCAGACATTTATACTCTGCATTTTGAAGCCAGTAATTTGCAAGATAC	50	0.16966406515100102	No Hit
ACTTATGGGACCTTGGAGAAGAAAAGGGGTTTATATGGTTTAAAATCCCGGTTTAT	46	0.15609093993892093	No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.1526976586359009	No Hit
ACTTATGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	45	0.1526976586359009	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	43	0.14591109602986085	No Hit
ACTTATGGGAAGGGAGATCACCACCAGTGAGCCTGGGAGCAGTGAGATGGGCGCTT	43	0.14591109602986085	No Hit
ACTTATGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	0.13912453342382083	No Hit
ACTTATGGGTGGGGACTCTTCTGAAGCTGTTGGATGAATATAGATTGTAGTGTGTG	40	0.1357312521208008	No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	40	0.1357312521208008	No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	39	0.13233797081778081	No Hit
ACTTATGGGAGAACATCACCTACCACTGCAAGAACAGCATTGCGTACCTGGATGAG	39	0.13233797081778081	No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	39	0.13233797081778081	No Hit
ACTTATGGGGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTAT	39	0.13233797081778081	No Hit
ACTTATGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.13233797081778081	No Hit
ACTTATGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37	0.12555140821174077	No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36	0.12215812690872074	No Hit
ACTTATGGGGTTGCTGCCGGGCAGTGTGTGGGGACTGGGGCTGGCAGCATTTGCCA	31	0.10519172039362064	No Hit
ACTTATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.10179843909060061	No Hit
ACTTATGGGGCTGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGAC	30	0.10179843909060061	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGATA	30	8.828265E-7	51.001743	49
ACAGAGC	15	0.0077184383	51.001743	50
AAGATAC	30	8.828265E-7	51.001743	50
AGTCGGA	20	3.731772E-4	51.001743	49
CCTCAAG	15	0.0077184383	51.001743	49
GTCGGAG	20	3.731772E-4	51.001743	50
CTGAGTC	20	3.9289647E-4	50.47414	46
AGCCAAA	15	0.008043179	50.47414	44
ACTGAGT	20	3.9289647E-4	50.47414	45
CTCCTCA	15	0.008043179	50.47414	47
TGCAAGA	30	9.4882853E-7	50.47414	47
GTTCTCC	15	0.008043179	50.47414	44
TGCTTGA	15	0.008043179	50.47414	44
TTCTCCT	15	0.008043179	50.47414	45
TACTGAG	20	3.9289647E-4	50.47414	44
AGGCATA	15	0.008043179	50.47414	46
TCTCCTC	15	0.008043179	50.47414	46
GCCAAAC	15	0.008043179	50.47414	45
TGAGTCG	20	3.9289647E-4	50.47414	47
TGTTCTC	15	0.008098275	50.387264	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content