FastQCFastQC Report
Fri 17 Jun 2016
SRR1526882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22811
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2551.1178817237297796No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.6751128841348473No Hit
CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.5085265880496251No Hit
CATGATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.35509184165534174No Hit
CATGATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.3331725921704441No Hit
CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.29371794309762833No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.2761825435097102No Hit
CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.26303099381877165No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.21480864495199686No Hit
CATGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.157818596291263No Hit
CATGATGGGATTGTTAGGTTCTCCTAACCTCTGTTGACTACTATGGTAAGTGATGTTATTTTAA350.15343474639428345No Hit
CATGATGGGATCGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.1446670466003244No Hit
CATGATGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.14028319670334488No Hit
CATGATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.13151549690938583No Hit
CATGATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG300.13151549690938583No Hit
CATGATGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.12713164701240628No Hit
CATGATGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.12274779711542677No Hit
CATGATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12274779711542677No Hit
CATGATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11398009732146772No Hit
CATGATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11398009732146772No Hit
CATGATGGGATCACAGCTCACCGCGAGGCCACTGCCTCTGAGCCACGTACCTTACTCTTGGTAC250.10959624742448819No Hit
CATGATGGGTAACTTTTCCTGGGTTTCATTCTTTCTAGTGTACTAGATCCAGAAATTTAGTGTG250.10959624742448819No Hit
CATGATGGGGGGAGTCTCATCATTGGCTATGCCAGGAACCCTTCTCTGAAGCAACAACTCTTCT250.10959624742448819No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT240.10521239752750866No Hit
CATGATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.10521239752750866No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT230.10082854763052912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA550.058.497785
TGATGGA501.8189894E-1258.497783
GATGGAA257.982944E-658.497784
ATGGGAC1300.058.497785
ATGGAAG257.982944E-658.497785
TGGGGGC257.982944E-658.497786
GGGGCTC650.058.497787
GGGGCCT257.982944E-658.497787
GGGAAGA257.982944E-658.497787
TGGGATT257.982944E-658.497786
TGGGACG257.982944E-658.497786
TGGGACA650.058.497786
ATGGGTT150.004473889758.4977765
ATGGGCT850.058.4977765
GGGAGAT303.391251E-758.4977767
TGGGGTC150.004473889758.4977766
TGGGGCT950.058.4977766
GGGATAT150.004473889758.4977767
TGGGTAT150.004473889758.4977766
GGGGTGC351.4455509E-858.4977767
Produced by FastQC (version 0.11.5)