FastQCFastQC Report
Fri 17 Jun 2016
SRR1526881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26244
Sequences flagged as poor quality0
Sequence length64
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4401.6765736930345985No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.6668190824569425No Hit
CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.5525072397500381No Hit
CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.411522633744856No Hit
CATGATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.3734186861758878No Hit
CATGATGGGATCACAGCTCACCGCGAGGCCACTGCCTCTGAGCCACGTACCTTACTCTTGGTAC810.30864197530864196No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT770.2934003962810547No Hit
CATGATGGGGGGAGTCTCATCATTGGCTATGCCAGGAACCCTTCTCTGAAGCAACAACTCTTCT770.2934003962810547No Hit
CATGATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG740.2819692120103643No Hit
CATGATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.2629172382258802No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.2591068434689834No Hit
CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.2591068434689834No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.2591068434689834No Hit
CATGATGGGAGCAAATGGGCATTTTACAAGAAGTACGAATCTTATTTTTCCTGTCCTGCCCCTG660.2514860539551898No Hit
CATGATGGGGATGCCTTAAACTTATGAGTAAGGAAAATAACGATTCGGGGTGACGCCCGAGTCC620.23624447492760248No Hit
CATGATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.18289894833104708No Hit
CATGATGGGGCTCTTTTCCCCTCGGAAGAGAAGAGGCGATGGCGGCGATGGCATCTTTCGGCGC470.1790885535741503No Hit
CATGATGGGCAAAACAAAACAAAACAAAACATGGAGAAGATGTTCCTCAGAAATATGGACTTCA440.16765736930345984No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA430.16384697454656302No Hit
CATGATGGGATTGTTAGGTTCTCCTAACCTCTGTTGACTACTATGGTAAGTGATGTTATTTTAA420.1600365797896662No Hit
CATGATGGGGCATTTCATTCTTTATCCTTGTGGCTGTCCTTGGGTCCCAGGCTGGCCTCATACA420.1600365797896662No Hit
CATGATGGGGGGCAAAATCTGATCACTATAAAGCATGATAGTCACTAAACAGAAGGGATTATAA410.1562261850327694No Hit
CATGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC390.14860539551897575No Hit
CATGATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG390.14860539551897575No Hit
CATGATGGGGTCGCTAACTCTTAACTCCCTGTCTGCATGAGCATGTGCTCTCCCTGTCCTCGCC360.1371742112482853No Hit
CATGATGGGCAGTGGATCTGTTGCTGGTTTTCTGCTACTGTTCGTCAGCTGAAGTCATTTTGCA350.1333638164913885No Hit
CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG340.1295534217344917No Hit
CATGATGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC340.1295534217344917No Hit
CATGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.1257430269775949No Hit
CATGATGGGGGACACACTTTTACATAGAAAGCTCTGCTCTCAAGGTACGGTGTTGCCTTCTAAT330.1257430269775949No Hit
CATGATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGTCC330.1257430269775949No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT320.12193263222069807No Hit
CATGATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.12193263222069807No Hit
CATGATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.12193263222069807No Hit
CATGATGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.11812223746380124No Hit
CATGATGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.11812223746380124No Hit
CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG280.1066910531931108No Hit
CATGATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.102880658436214No Hit
CATGATGGGATATAACAAAAATAAATGACTGGGTGATTTCTGGTATATCATGAGAGGCTTTTTT270.102880658436214No Hit
CATGATGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.102880658436214No Hit
CATGATGGGAGACACACCTTCCTTTCAGCTCTCTGCTGCACTGGTGCGACACCTCTGATTCCTC270.102880658436214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAA201.9287401E-458.2716844
GAGCGTG150.00455089558.2716851
TGGCATC150.00455089558.2716849
AACAACT201.9287401E-458.2716853
CGGAGCG201.9287401E-458.2716849
TTTCCAC150.00455089558.2716848
TATGTTC150.00455089558.2716855
CATCTTT150.00455089558.2716852
CAACTCT201.9287401E-458.2716855
GTTCTAT150.00455089558.2716858
TGTTCTA150.00455089558.2716857
TAGCCCA150.00455089558.2716841
GACGGAG201.9287401E-458.2716847
ATGGCAT150.00455089558.2716848
ACTCTTC201.9287401E-458.2716857
CATTCAG258.203813E-658.2716841
CAACAAC201.9287401E-458.2716852
ACATTCA258.203813E-658.2716840
GCGTGCT150.00455089558.2716853
CATTAAA150.00455089558.2716848
Produced by FastQC (version 0.11.5)