FastQCFastQC Report
Fri 17 Jun 2016
SRR1526880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26326
Sequences flagged as poor quality0
Sequence length64
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4321.6409633062371798No Hit
CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.5811745042923345No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.5469877687457266No Hit
CATGATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.36845703866899643No Hit
CATGATGGGGGGAGTCTCATCATTGGCTATGCCAGGAACCCTTCTCTGAAGCAACAACTCTTCT860.32667325077869785No Hit
CATGATGGGATCACAGCTCACCGCGAGGCCACTGCCTCTGAGCCACGTACCTTACTCTTGGTAC850.32287472460685257No Hit
CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.28109093671655394No Hit
CATGATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.27729241054470866No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT690.26209830585732735No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.25829977968548207No Hit
CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.24310567499810073No Hit
CATGATGGGGATGCCTTAAACTTATGAGTAAGGAAAATAACGATTCGGGGTGACGCCCGAGTCC630.23930714882625542No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.21651599179518347No Hit
CATGATGGGAGCAAATGGGCATTTTACAAGAAGTACGAATCTTATTTTTCCTGTCCTGCCCCTG540.20512041327964747No Hit
CATGATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG510.19372483476411154No Hit
CATGATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG500.1899263085922662No Hit
CATGATGGGGCATTTCATTCTTTATCCTTGTGGCTGTCCTTGGGTCCCAGGCTGGCCTCATACA440.16713515156119427No Hit
CATGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.15573957304565827No Hit
CATGATGGGATTGTTAGGTTCTCCTAACCTCTGTTGACTACTATGGTAAGTGATGTTATTTTAA410.15573957304565827No Hit
CATGATGGGGGGCAAAATCTGATCACTATAAAGCATGATAGTCACTAAACAGAAGGGATTATAA370.14054546835827697No Hit
CATGATGGGCAAAACAAAACAAAACAAAACATGGAGAAGATGTTCCTCAGAAATATGGACTTCA360.13674694218643166No Hit
CATGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC340.12914988984274103No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA340.12914988984274103No Hit
CATGATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.12914988984274103No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT330.1253513636708957No Hit
CATGATGGGTAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.1253513636708957No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC320.12155283749905037No Hit
CATGATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.12155283749905037No Hit
CATGATGGGATATAACAAAAATAAATGACTGGGTGATTTCTGGTATATCATGAGAGGCTTTTTT320.12155283749905037No Hit
CATGATGGGGCTCTTTTCCCCTCGGAAGAGAAGAGGCGATGGCGGCGATGGCATCTTTCGGCGC310.11775431132720504No Hit
CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG310.11775431132720504No Hit
CATGATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGTCC310.11775431132720504No Hit
CATGATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGCTAGTAG300.11395578515535972No Hit
CATGATGGGATCGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.11015725898351439No Hit
CATGATGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.11015725898351439No Hit
CATGATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC290.11015725898351439No Hit
CATGATGGGGTCGCTAACTCTTAACTCCCTGTCTGCATGAGCATGTGCTCTCCCTGTCCTCGCC280.10635873281166906No Hit
CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG270.10256020663982374No Hit
CATGATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.10256020663982374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAA150.0045283458.3454944
GCTAGGC150.0045283458.3454939
CGCTTGC150.0045283458.3454939
GCTATGC150.0045283458.3454926
CTAGGCT150.0045283458.3454940
CTCAGTG258.14337E-658.3454926
GGCTCAC150.0045283458.3454943
GCTTGCC150.0045283458.3454940
GGCTATG150.0045283458.3454925
TCTCTGA150.0045283458.3454943
GCTCAGT258.14337E-658.3454925
GCTCACA150.0045283458.3454944
TAGGCTC150.0045283458.3454941
CATTGGC150.00456294858.23371521
AGCTCAG150.00456294858.23371524
ATTGGCT150.00456294858.23371522
TAGCTCA150.00456294858.23371523
TGTCAGG150.00456294858.23371521
TTGGCTA150.00456294858.23371523
TCATTGG150.00456294858.23371520
Produced by FastQC (version 0.11.5)