FastQCFastQC Report
Fri 17 Jun 2016
SRR1526879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22644
Sequences flagged as poor quality0
Sequence length56
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4722.0844373785550254No Hit
CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.8567390920332096No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.7419183889772125No Hit
CATGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1380.6094329623741388No Hit
CATGATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.4062886415827592No Hit
CATGATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT900.39745627980922094No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG700.30913266207383855No Hit
CATGATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.3003003003003003No Hit
CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.29146793852676206No Hit
CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.29146793852676206No Hit
CATGATGGGGGGAGTCTCATCATTGGCTATGCCAGGAACCCTTCTCTGAAGCAACA660.29146793852676206No Hit
CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.28705175763999297No Hit
CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA620.27380321497968557No Hit
CATGATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG580.2561384914326091No Hit
CATGATGGGATCACAGCTCACCGCGAGGCCACTGCCTCTGAGCCACGTACCTTACT570.25172231054584No Hit
CATGATGGGGATGCCTTAAACTTATGAGTAAGGAAAATAACGATTCGGGGTGACGC510.22522522522522523No Hit
CATGATGGGATTGTTAGGTTCTCCTAACCTCTGTTGACTACTATGGTAAGTGATGT470.20756050167814877No Hit
CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG430.18989577813107225No Hit
CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.181063416357534No Hit
CATGATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG400.17664723547076489No Hit
CATGATGGGCAAAACAAAACAAAACAAAACATGGAGAAGATGTTCCTCAGAAATAT400.17664723547076489No Hit
CATGATGGGGGGCAAAATCTGATCACTATAAAGCATGATAGTCACTAAACAGAAGG400.17664723547076489No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC400.17664723547076489No Hit
CATGATGGGGCTCTTTTCCCCTCGGAAGAGAAGAGGCGATGGCGGCGATGGCATCT390.17223105458399576No Hit
CATGATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG370.16339869281045752No Hit
CATGATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1589825119236884No Hit
CATGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG350.15456633103691927No Hit
CATGATGGGGCATTTCATTCTTTATCCTTGTGGCTGTCCTTGGGTCCCAGGCTGGC350.15456633103691927No Hit
CATGATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.15015015015015015No Hit
CATGATGGGAGCAAATGGGCATTTTACAAGAAGTACGAATCTTATTTTTCCTGTCC340.15015015015015015No Hit
CATGATGGGATATAACAAAAATAAATGACTGGGTGATTTCTGGTATATCATGAGAG340.15015015015015015No Hit
CATGATGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.15015015015015015No Hit
CATGATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.14573396926338103No Hit
CATGATGGGTAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.13248542660307366No Hit
CATGATGGGATCGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.12806924571630454No Hit
CATGATGGGCCTTGTCGCCGCCAAGAACAAGATGGCCGGGAGAATCTCTGCCTTCC290.12806924571630454No Hit
CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA280.12365306482953542No Hit
CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC270.11923688394276628No Hit
CATGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.11923688394276628No Hit
CATGATGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA270.11923688394276628No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA260.11482070305599718No Hit
CATGATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG250.11040452216922805No Hit
CATGATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11040452216922805No Hit
CATGATGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.11040452216922805No Hit
CATGATGGGATTCGAGGGAAGTACTCTACCAACCTAGTTATATCCCCAGCATCCCA240.10598834128245893No Hit
CATGATGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.10598834128245893No Hit
CATGATGGGTGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.10598834128245893No Hit
CATGATGGGGCTTTCTCTTCTCCATTCCTCCGCCATCGTGCGCGCTATTCTTGATT230.1015721603956898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGG150.007677993751.02954549
CCAGGCT150.00802994150.45617747
TTGGGTC150.008101745550.3430540
TCCTTGG150.00817402650.23042337
CCTTGGG150.00817402650.23042338
TCCTTGT150.00824678750.118325
AAACTCT150.00824678750.118334
TGTCCTT150.00824678750.118335
CCTTGTG150.00824678750.118326
AACTCTA150.00824678750.118335
GTCCTTG150.00824678750.118336
CTTGTGG150.00824678750.118327
GATCAAA150.00824678750.118330
ATCAAAC150.00824678750.118331
CTGTCCT150.00824678750.118334
TGGCTGT150.00824678750.118331
TCAAACT150.00832002750.0066832
CAAACTC150.00832002750.0066833
ATCCTTG150.008393750549.89555424
ATGGGTT301.0487347E-649.6747785
Produced by FastQC (version 0.11.5)