##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526879_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22644 Sequences flagged as poor quality 0 Sequence length 56 %GC 38 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.63297120650062 39.0 38.0 40.0 36.0 40.0 2 38.02477477477478 39.0 38.0 40.0 35.0 40.0 3 38.418433139021374 39.0 38.0 40.0 36.0 40.0 4 38.23679561914856 39.0 38.0 40.0 35.0 40.0 5 37.46789436495319 39.0 38.0 40.0 33.0 40.0 6 38.31390213743155 39.0 38.0 40.0 35.0 40.0 7 38.50596184419714 39.0 38.0 40.0 36.0 40.0 8 38.51881293057764 39.0 38.0 40.0 36.0 40.0 9 38.47098569157393 39.0 38.0 40.0 36.0 40.0 10 37.77534887829005 39.0 38.0 40.0 33.0 40.0 11 37.88866807984455 39.0 38.0 40.0 35.0 40.0 12 37.832008479067305 39.0 38.0 40.0 35.0 40.0 13 37.549549549549546 39.0 38.0 40.0 33.0 40.0 14 37.8046723193782 39.0 38.0 40.0 34.0 40.0 15 37.81955484896661 39.0 38.0 40.0 34.0 40.0 16 37.642068539127365 39.0 38.0 40.0 34.0 40.0 17 37.61552729199788 39.0 38.0 40.0 33.0 40.0 18 37.53687511040452 39.0 38.0 40.0 33.0 40.0 19 37.322425366543015 39.0 37.0 40.0 33.0 40.0 20 37.38866807984455 39.0 37.0 40.0 33.0 40.0 21 37.30926514750044 39.0 37.0 40.0 33.0 40.0 22 37.174174174174176 39.0 37.0 40.0 33.0 40.0 23 37.00017664723547 39.0 36.0 40.0 33.0 40.0 24 36.86720544073485 39.0 36.0 40.0 32.0 40.0 25 36.8101483836778 39.0 36.0 40.0 32.0 40.0 26 36.47774244833068 39.0 36.0 40.0 31.0 40.0 27 36.5057410351528 39.0 36.0 40.0 31.0 40.0 28 36.28113407525172 38.0 36.0 40.0 31.0 40.0 29 36.326797385620914 39.0 36.0 40.0 31.0 40.0 30 36.12599364069953 38.0 35.0 40.0 31.0 40.0 31 35.88787316728493 39.0 35.0 40.0 30.0 40.0 32 35.606606606606604 39.0 35.0 40.0 29.0 40.0 33 35.660086557145384 39.0 35.0 40.0 29.0 40.0 34 35.75406288641583 39.0 35.0 40.0 29.0 40.0 35 35.55657127715951 39.0 35.0 40.0 29.0 40.0 36 35.60082140964494 39.0 35.0 40.0 29.0 40.0 37 35.434728846493556 39.0 35.0 40.0 29.0 40.0 38 35.21533298003886 39.0 35.0 40.0 28.0 40.0 39 35.11526232114468 39.0 35.0 40.0 27.0 40.0 40 35.06712594947889 39.0 35.0 40.0 27.0 40.0 41 34.64842783960431 39.0 35.0 40.0 25.0 40.0 42 34.49244833068362 39.0 35.0 40.0 23.0 40.0 43 34.463389860448686 39.0 35.0 40.0 24.0 40.0 44 34.2357798975446 39.0 34.0 40.0 23.0 40.0 45 33.86486486486486 39.0 34.0 40.0 18.0 40.0 46 33.5229641406112 39.0 34.0 40.0 10.0 40.0 47 33.33907436848613 38.0 33.0 39.0 8.0 40.0 48 33.15765765765766 38.0 33.0 39.0 4.0 40.0 49 32.80127186009539 38.0 33.0 39.0 4.0 40.0 50 32.53276806217983 38.0 32.0 39.0 4.0 40.0 51 31.86914856032503 38.0 32.0 39.0 4.0 40.0 52 31.666887475711004 38.0 32.0 39.0 4.0 40.0 53 31.294117647058822 37.0 31.0 39.0 4.0 40.0 54 30.749735029146795 37.0 30.0 39.0 4.0 40.0 55 30.2959282812224 36.0 30.0 39.0 4.0 40.0 56 17.93075428369546 21.0 15.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.1628796815209625 1 2 -1.9308486922100343 1 3 -1.3343847426838522 1 4 -1.222191560618569 1 5 -2.9651449127193885 1 6 -2.442450582157356 1 7 -1.3534133412254192 1 8 -1.492151325799533 1 9 -1.1390940053501524 1 10 -1.693839025855354 1 11 -0.9154931727786817 1 12 -1.2434303363207562 1 13 -2.5392004848497294 1 14 -1.2980894849240983 1 15 -1.5584070732965074 1 16 -2.3111261356029402 1 17 -3.2640966452906213 1 18 -1.7844822345250861 1 19 -1.667099685789367 1 20 -2.5714570997861728 1 21 -0.6029187028074645 1 22 -1.1807061137087231 1 23 -0.8325865265157901 1 24 -0.7221760238214401 1 25 -0.7640991906701089 1 26 -0.6104764565754408 1 27 -0.1882223061971402 1 28 -1.1668567274150234 1 29 -0.9103713175207488 1 30 0.18676585481412644 1 31 -0.3651017783774151 1 32 -1.9160186634523697 1 33 -0.7071782671511571 1 34 -0.5694010317979874 1 35 -1.4756835095790066 1 36 0.568301384165963 1 37 0.3710360110424702 1 38 0.484481389132533 1 39 0.6680885225760349 1 40 1.0775606616235507 1 41 0.2583734177355268 1 42 0.20263933069893625 1 43 0.7100658754305584 1 44 0.07992281125423517 1 45 0.91513031641027 1 46 0.3953352694985881 1 47 1.4416923525080918 1 48 1.5360071571958116 1 49 1.0794535925948026 1 50 0.30978167779306887 1 51 0.10866105571683704 1 52 0.2709594916255007 1 53 -0.045526865538853656 1 54 1.2425758145661199 1 55 1.3687770385180542 1 56 2.4017723286404618 2 1 -0.7462130148542983 2 2 -1.51418202554337 2 3 -1.042718076017188 2 4 -1.972191560618569 2 5 -1.0901449127193885 2 6 -0.6924505821573561 2 7 -0.7700800078920835 2 8 -1.2004846591328615 2 9 -1.097427338683488 2 10 -0.9855056925220183 2 11 -2.5404931727786817 2 12 -0.9517636696540848 2 13 -1.3308671515164008 2 14 -0.38142281825743396 2 15 -0.2250737399631717 2 16 -0.22779280226960452 2 17 -1.3057633119572856 2 18 -0.36781556785841474 2 19 -0.542099685789367 2 20 -0.5714570997861728 2 21 -1.1445853694741288 2 22 -0.5973727803753874 2 23 -0.04091985984912583 2 24 0.19449064284523132 2 25 -0.6390991906701089 2 26 0.3478568767578949 2 27 0.5201110271361955 2 28 -1.9006074835914433E-4 2 29 0.7562953491459155 2 30 -0.47990081185253786 2 31 -1.115101778377415 2 32 0.042314669880965994 2 33 -0.8321782671511571 2 34 -0.8194010317979874 2 35 -1.2256835095790066 2 36 -0.18169861583403701 2 37 -0.5039639889575298 2 38 0.6928147224658687 2 39 -0.1235781440906365 2 40 0.7025606616235507 2 41 0.0083734177355268 2 42 -0.17236066930106375 2 43 0.33506587543055844 2 44 -0.7117438554124291 2 45 0.29013031641027 2 46 0.6870019361652524 2 47 1.1500256858414204 2 48 1.7443404905291473 2 49 2.2044535925948026 2 50 2.3931150111264046 2 51 1.9419943890501727 2 52 1.3959594916255043 2 53 0.8294731344611499 2 54 1.8259091478994556 2 55 1.0354437051847185 2 56 0.6101056619737939 3 1 0.1371203184790346 3 2 0.3841513077899634 3 3 0.5422819239828129 3 4 -0.2638582272852332 3 5 -0.2651449127193857 3 6 0.32254941784264446 3 7 0.4399199921079173 3 8 0.3061820075338062 3 9 0.36757266131651534 3 10 0.5444943074779829 3 11 0.6095068272213169 3 12 0.5349029970125798 3 13 1.502466181816935 3 14 1.1219105150759034 3 15 0.9149262600368289 3 16 1.0338738643970586 3 17 1.4042366880427153 3 18 1.3488510988082467 3 19 1.427900314210632 3 20 1.3168762335471627 3 21 0.09041463052587062 3 22 1.1392938862912771 3 23 1.284080140150877 3 24 1.316157309511894 3 25 0.7142341426632299 3 26 1.119523543424556 3 27 -0.8748889728638076 3 28 0.8748099392516409 3 29 -0.20537131752075055 3 30 0.6167658548141262 3 31 -0.585101778377414 3 32 -0.06268533011903088 3 33 0.8144883995155041 3 34 -0.17606769846464942 3 35 1.4359831570876551 3 36 -0.11669861583403929 3 37 0.34103601104246906 3 38 0.33781472246587185 3 39 -1.5252448107573002 3 40 -1.209106005043111 3 41 -1.094959915597812 3 42 -1.625694002634397 3 43 -2.456600791236106 3 44 -2.1750771887457674 3 45 -3.8315363502563926 3 46 -2.0329980638347465 3 47 -2.341640980825243 3 48 -0.6856595094708524 3 49 -2.6305464074051983 3 50 -2.32021832220693 3 51 -1.4463389442831627 3 52 -1.8023738417078299 3 53 -1.8938601988721864 3 54 -0.592424185433881 3 55 -1.532889628148613 3 56 0.35010566197379234 4 1 -0.07197059061186906 4 2 -0.4649396013009408 4 3 -0.6904453487444613 4 4 -0.4456764091034131 4 5 -1.056054003628482 4 6 -0.13563240033917623 4 7 0.025374537562463217 4 8 0.14800018935198267 4 9 0.027572661316511926 4 10 -0.09914205615838512 4 11 0.8913250090394982 4 12 0.12399390610348604 4 13 1.2297389090896615 4 14 0.7928196059849952 4 15 -0.27052828541771845 4 16 0.26841931894251303 4 17 -0.10121785741183231 4 18 0.639760189717343 4 19 -0.2466451403348202 4 20 0.45505805172898306 4 21 0.8213237214349647 4 22 0.41020297720037036 4 23 1.0840801401508742 4 24 -0.3547517813971979 4 25 0.6942341426632268 4 26 1.3667962706972858 4 27 1.054201936227102 4 28 0.8293553937970941 4 29 0.7146286824792512 4 30 1.3004022184504933 4 31 0.27126185798621805 4 32 0.917314669880966 4 33 1.5617611267882339 4 34 2.0821141197171684 4 35 0.9978013389058376 4 36 -0.8294258885613104 4 37 -0.19714580713934993 4 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0.7732398699107961 8 21 0.9576873577985978 8 22 1.137475704473097 8 23 1.0840801401508742 8 24 0.23615730951189562 8 25 0.9669614153905002 8 26 0.0031599070609260593 8 27 -0.12761624559107787 8 28 -0.03428096983926565 8 29 -0.012644044793475473 8 30 -0.7450523270040534 8 31 0.36217094889531154 8 32 -0.6735944210281275 8 33 0.5617611267882339 8 34 0.3548413924444418 8 35 1.1341649752694778 8 36 -0.8294258885613104 8 37 1.484672374678837 8 38 1.4087238133749622 8 39 1.016573371060879 8 40 0.638166722229613 8 41 3.1977673571294645 8 42 1.585215088274694 8 43 0.8615810269457143 8 44 2.932195538526962 8 45 2.4757363770163323 8 46 3.4597292088925258 8 47 3.4947226555383892 8 48 3.4488859450746006 8 49 3.9658172289584357 8 50 3.877963495974889 8 51 3.097297419353204 8 52 3.346717067383075 8 53 3.473412528400541 8 54 2.7312121782024867 8 55 0.8271103718513864 8 56 1.6555602074283406 9 1 -0.1174251360664158 9 2 0.48960585324451245 9 3 0.35500919671008546 9 4 -0.3547673181943267 9 5 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0.9009646162294516 117 9 0.9623552700121607 117 10 1.5253638726953724 117 11 0.7312459576560997 117 12 1.4322943013604075 117 13 0.9372487905125837 117 14 1.0714757324672064 117 15 1.2314479991672656 117 16 0.7486564730927086 117 17 0.5746714706514098 117 18 0.7227641422865076 117 19 0.925291618558461 117 20 0.5933979726776002 117 21 -0.8782810216480428 117 22 -0.5285322006652464 117 23 -0.30722420767521186 117 24 -0.633407907879409 117 25 -0.6318528138585151 117 26 -0.6213460217928315 117 27 -2.1374976685159766 117 28 -1.6469291911831405 117 29 -1.6766756653468349 117 30 -1.5414950147510922 117 31 -0.7781452566382825 117 32 -0.8870331562059874 117 33 -1.0568159483105788 117 34 -1.4317198723776983 117 35 -0.553582060303647 117 36 0.1824318189485723 117 37 -0.2920074672183972 117 38 -0.008272234055873184 117 39 0.09562475446008989 117 40 -0.39345383113006704 117 41 -0.09669904603259027 117 42 0.14647991040907726 117 43 -0.5000790521056686 117 44 -0.5559467539631555 117 45 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-0.08352339408169485 120 29 0.29796201581258686 120 30 0.06176585481412644 120 31 1.384898221622585 120 32 -0.04101866345236971 120 33 0.37615506618217154 120 34 0.847265634868684 120 35 1.3993164904209934 120 36 1.1516347174992987 120 37 1.579369344375806 120 38 2.0261480557992044 120 39 1.7097551892426992 120 40 0.8275606616235507 120 41 2.8417067510688554 120 42 1.8276393306989362 120 43 1.9600658754305584 120 44 2.2465894779208995 120 45 0.8734636497436057 120 46 0.020335269498588104 120 47 -0.1833076474919082 120 48 -0.8806595094708527 120 49 0.9961202592614669 120 50 -0.5235516555402633 120 51 -1.9330056109498308 120 52 -2.8957071750411636 120 53 -2.462193532205518 120 54 -0.799090852100548 120 55 0.4937770385180542 120 56 0.568438995307126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 17.0 5 2.0 6 3.0 7 1.0 8 10.0 9 15.0 10 12.0 11 31.0 12 7.0 13 26.0 14 39.0 15 48.0 16 52.0 17 87.0 18 90.0 19 146.0 20 121.0 21 133.0 22 156.0 23 139.0 24 166.0 25 189.0 26 209.0 27 251.0 28 313.0 29 352.0 30 430.0 31 553.0 32 711.0 33 818.0 34 1156.0 35 1482.0 36 2022.0 37 3417.0 38 4990.0 39 4450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.1697579932874 0.6226815050344462 0.03532944709415298 0.17223105458399576 8 99.14722516790385 0.5832449628844115 0.18115942028985507 0.08837044892188052 9 96.4405582052641 1.6030736618971912 0.6800918565624448 1.2762762762762763 10 33.50556438791733 41.41494435612083 8.986928104575163 16.09256315138668 11 24.02844020491079 34.388800565271154 20.367426249779193 21.215332980038863 12 40.58470234940823 17.934110581169406 23.820879703232645 17.66030736618972 13 23.595654478007418 17.262851086380497 30.29058470234941 28.850909733262675 14 25.225225225225223 27.230171347818406 28.68309485956545 18.86150856739092 15 30.374492139198022 23.03038332450097 26.5589118530295 20.036212683271508 16 22.363540010598832 25.24730612965907 28.245892951775303 24.14326090796679 17 26.06871577459813 24.134428546193252 22.001413177883766 27.795442501324853 18 25.922981805334743 26.179120296767355 24.019607843137255 23.878290054760644 19 22.659424130012365 26.377848436671965 26.651651651651655 24.31107578166402 20 21.113760819643172 28.59918742271683 23.02155096272743 27.265500794912562 21 22.03674262497792 24.83218512630277 28.100158982511925 25.03091326620738 22 23.410174880763115 23.904787140081257 26.104045221692278 26.580992757463346 23 27.397986221515637 27.799858682211624 26.21003356297474 18.592121533298002 24 23.00490217727333 30.927880581195073 26.635163185090317 19.432054056441284 25 25.905637038345997 23.27708075631737 29.258702951051426 21.55857925428521 26 25.926907949975696 23.085421361969154 29.179371602810555 21.8082990852446 27 21.179849756959786 20.680512593901902 31.948740609809988 26.190897039328327 28 20.23609514545937 25.952780970908123 34.57423291184013 19.236890971792377 29 22.030973451327434 21.216814159292035 36.96017699115045 19.792035398230087 30 18.447978030739247 24.22819683748948 40.5412588032068 16.782566328564467 31 19.721680553093424 23.41783371742599 41.09643680198546 15.764048927495125 32 15.006204573657152 21.18861903917745 48.90533593334515 14.899840453820246 33 16.754576481538937 20.13651877133106 46.83303045077789 16.27587429635211 34 16.00744945015963 22.978006385242995 48.65200425682866 12.362539907768712 35 14.349079618540697 25.136393878908848 49.28365491239743 11.230871590153027 36 15.751175791995742 23.10764042949685 47.47981187328068 13.661371905226726 37 13.96566257042722 22.825074309036868 50.370436094228296 12.838827026307618 38 13.810855190165535 23.028447166378204 51.772955221231086 11.38774242222518 39 12.463935372186958 22.681876692263305 52.62994362821253 12.224244307337209 40 14.715421868751942 22.966188296974273 51.46843204336429 10.849957790909496 41 12.950535531754145 22.327896537931647 52.49544464690457 12.226123283409626 42 12.996133161473844 21.969865327347883 52.70900928930175 12.324992221876528 43 13.35735254870563 21.323725647184414 52.62432167956588 12.694600124544081 44 13.738047587280407 21.44096064042695 52.381587725150105 12.43940404714254 45 12.409343715239155 23.78197997775306 52.33815350389322 11.470522803114571 46 13.09804619698251 22.08821042324981 50.05563220437047 14.758111175397215 47 13.00921597435555 23.86803793241619 51.19985753083122 11.922888562397043 48 13.614500757103412 23.92001425135833 49.80404382292687 12.661441168611384 49 11.901157613535174 27.337488869100625 49.759572573463934 11.001780943900268 50 13.395402708481823 26.585887384176765 49.32733428367783 10.691375623663578 51 12.468827930174564 26.44727467046669 50.12468827930174 10.959209120057 52 13.217213114754097 26.49233784746971 48.03545972915182 12.254989308624378 53 12.624777183600713 28.58288770053476 47.606951871657756 11.185383244206774 54 13.81894888021184 27.39553879987433 48.02746734886226 10.758044971051568 55 13.046579006017383 27.742366837530646 46.146645865834635 13.064408290617338 56 13.245977626242366 27.08918304586175 47.065115657173415 12.599723670722469 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 2.0 5 3.0 6 4.0 7 5.0 8 47.5 9 90.0 10 92.0 11 94.0 12 100.0 13 130.0 14 154.0 15 294.5 16 435.0 17 500.0 18 565.0 19 539.5 20 514.0 21 619.0 22 873.5 23 1023.0 24 1214.5 25 1406.0 26 1539.0 27 1672.0 28 1744.0 29 1669.5 30 1523.0 31 1221.0 32 919.0 33 973.0 34 1027.0 35 688.5 36 350.0 37 324.5 38 304.5 39 310.0 40 373.5 41 437.0 42 399.0 43 361.0 44 459.5 45 558.0 46 519.5 47 509.0 48 537.0 49 564.5 50 592.0 51 706.0 52 820.0 53 821.5 54 786.0 55 749.0 56 843.5 57 938.0 58 936.5 59 935.0 60 775.5 61 616.0 62 550.0 63 452.0 64 420.0 65 402.0 66 384.0 67 278.0 68 172.0 69 142.5 70 113.0 71 98.0 72 68.5 73 54.0 74 37.5 75 21.0 76 19.5 77 18.0 78 13.0 79 4.5 80 1.0 81 1.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.052994170641229466 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004416180886769122 25 0.03532944709415298 26 0.06624271330153683 27 0.06182653241476771 28 0.11482070305599718 29 0.19431195901784137 30 0.29588411941353115 31 0.35329447094152977 32 0.35329447094152977 33 0.36654301360183716 34 0.4062886415827592 35 0.43720190779014306 36 0.4681151739975269 37 0.4548666313372196 38 0.49019607843137253 39 0.507860801978449 40 0.6050167814873697 41 0.6315138668079845 42 0.6403462285815227 43 0.7154213036565978 44 0.7021727609962904 45 0.7463345698639817 46 0.7728316551845964 47 0.8081611022787493 48 0.8390743684861331 49 0.8125772831655184 50 0.8655714538067479 51 0.8302420067125951 52 0.8655714538067479 53 0.9009009009009009 54 1.6030736618971912 55 0.9229818053347464 56 0.9141494435612083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 22644.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.693340399222755 #Duplication Level Percentage of deduplicated Percentage of total 1 71.29540900775328 36.14202437731849 2 14.04303510758777 14.237767178943649 3 5.444725150274414 8.280339162692103 4 2.874814879344891 5.829358770535241 5 1.803292969770886 4.5707472178060415 6 1.1934837529401516 3.630100688924218 7 0.8188866626012719 2.9058470234940823 8 0.5923860963498563 2.4024024024024024 9 0.2613468072131719 1.192368839427663 >10 1.533234602317275 12.93499381734676 >50 0.10453872288526875 3.5815226991697577 >100 0.03484624096175625 4.292527821939586 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 472 2.0844373785550254 No Hit CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 194 0.8567390920332096 No Hit CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168 0.7419183889772125 No Hit CATGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 138 0.6094329623741388 No Hit CATGATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.4062886415827592 No Hit CATGATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 90 0.39745627980922094 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 70 0.30913266207383855 No Hit CATGATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.3003003003003003 No Hit CATGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.29146793852676206 No Hit CATGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.29146793852676206 No Hit CATGATGGGGGGAGTCTCATCATTGGCTATGCCAGGAACCCTTCTCTGAAGCAACA 66 0.29146793852676206 No Hit CATGATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 0.28705175763999297 No Hit CATGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 62 0.27380321497968557 No Hit CATGATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 58 0.2561384914326091 No Hit CATGATGGGATCACAGCTCACCGCGAGGCCACTGCCTCTGAGCCACGTACCTTACT 57 0.25172231054584 No Hit CATGATGGGGATGCCTTAAACTTATGAGTAAGGAAAATAACGATTCGGGGTGACGC 51 0.22522522522522523 No Hit CATGATGGGATTGTTAGGTTCTCCTAACCTCTGTTGACTACTATGGTAAGTGATGT 47 0.20756050167814877 No Hit CATGATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 43 0.18989577813107225 No Hit CATGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41 0.181063416357534 No Hit CATGATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 40 0.17664723547076489 No Hit CATGATGGGCAAAACAAAACAAAACAAAACATGGAGAAGATGTTCCTCAGAAATAT 40 0.17664723547076489 No Hit CATGATGGGGGGCAAAATCTGATCACTATAAAGCATGATAGTCACTAAACAGAAGG 40 0.17664723547076489 No Hit CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 40 0.17664723547076489 No Hit CATGATGGGGCTCTTTTCCCCTCGGAAGAGAAGAGGCGATGGCGGCGATGGCATCT 39 0.17223105458399576 No Hit CATGATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG 37 0.16339869281045752 No Hit CATGATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.1589825119236884 No Hit CATGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 35 0.15456633103691927 No Hit CATGATGGGGCATTTCATTCTTTATCCTTGTGGCTGTCCTTGGGTCCCAGGCTGGC 35 0.15456633103691927 No Hit CATGATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.15015015015015015 No Hit CATGATGGGAGCAAATGGGCATTTTACAAGAAGTACGAATCTTATTTTTCCTGTCC 34 0.15015015015015015 No Hit CATGATGGGATATAACAAAAATAAATGACTGGGTGATTTCTGGTATATCATGAGAG 34 0.15015015015015015 No Hit CATGATGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.15015015015015015 No Hit CATGATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33 0.14573396926338103 No Hit CATGATGGGTAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.13248542660307366 No Hit CATGATGGGATCGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.12806924571630454 No Hit CATGATGGGCCTTGTCGCCGCCAAGAACAAGATGGCCGGGAGAATCTCTGCCTTCC 29 0.12806924571630454 No Hit CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 28 0.12365306482953542 No Hit CATGATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 27 0.11923688394276628 No Hit CATGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.11923688394276628 No Hit CATGATGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.11923688394276628 No Hit CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 26 0.11482070305599718 No Hit CATGATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 25 0.11040452216922805 No Hit CATGATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.11040452216922805 No Hit CATGATGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.11040452216922805 No Hit CATGATGGGATTCGAGGGAAGTACTCTACCAACCTAGTTATATCCCCAGCATCCCA 24 0.10598834128245893 No Hit CATGATGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.10598834128245893 No Hit CATGATGGGTGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.10598834128245893 No Hit CATGATGGGGCTTTCTCTTCTCCATTCCTCCGCCATCGTGCGCGCTATTCTTGATT 23 0.1015721603956898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.004416180886769122 0.0 0.0 0.0 0.0 10 0.013248542660307366 0.0 0.0 0.0 0.0 11 0.03532944709415298 0.0 0.0 0.0 0.0 12 0.03532944709415298 0.0 0.0 0.0 0.0 13 0.03532944709415298 0.0 0.0 0.0 0.0 14 0.04857798975446034 0.0 0.0 0.0 0.0 15 0.052994170641229466 0.0 0.0 0.0 0.0 16 0.05741035152799859 0.0 0.0 0.0 0.0 17 0.05741035152799859 0.0 0.0 0.0 0.0 18 0.07065889418830595 0.0 0.0 0.0 0.0 19 0.07507507507507508 0.0 0.0 0.0 0.0 20 0.0794912559618442 0.0 0.0 0.0 0.0 21 0.08832361773538244 0.0 0.0 0.0 0.0 22 0.1192368839427663 0.0 0.0 0.0 0.0 23 0.13248542660307366 0.0 0.0 0.0 0.0 24 0.13690160748984279 0.0 0.0 0.0 0.0 25 0.14573396926338103 0.0 0.0 0.0 0.0 26 0.14573396926338103 0.0 0.0 0.0 0.0 27 0.15015015015015015 0.0 0.0 0.0 0.0 28 0.17223105458399576 0.0 0.0 0.0 0.0 29 0.17664723547076489 0.0 0.0 0.0 0.0 30 0.18547959724430313 0.0 0.0 0.0 0.0 31 0.18547959724430313 0.0 0.0 0.0 0.0 32 0.18547959724430313 0.0 0.0 0.0 0.0 33 0.18989577813107225 0.0 0.0 0.0 0.0 34 0.20314432079137962 0.0 0.0 0.0 0.0 35 0.2561384914326091 0.0 0.0 0.0 0.0 36 0.2561384914326091 0.0 0.0 0.0 0.0 37 0.2649708532061473 0.0 0.0 0.0 0.0 38 0.27380321497968557 0.0 0.0 0.0 0.0 39 0.2870517576399929 0.0 0.0 0.0 0.0 40 0.29588411941353115 0.0 0.0 0.0 0.0 41 0.3223812047341459 0.0 0.0 0.0 0.0 42 0.33121356650768413 0.0 0.0 0.0 0.0 43 0.3444621091679915 0.0 0.0 0.0 0.0 44 0.35329447094152977 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCTGG 15 0.0076779937 51.029545 49 CCAGGCT 15 0.008029941 50.456177 47 TTGGGTC 15 0.0081017455 50.34305 40 TCCTTGG 15 0.008174026 50.230423 37 CCTTGGG 15 0.008174026 50.230423 38 TCCTTGT 15 0.008246787 50.1183 25 AAACTCT 15 0.008246787 50.1183 34 TGTCCTT 15 0.008246787 50.1183 35 CCTTGTG 15 0.008246787 50.1183 26 AACTCTA 15 0.008246787 50.1183 35 GTCCTTG 15 0.008246787 50.1183 36 CTTGTGG 15 0.008246787 50.1183 27 GATCAAA 15 0.008246787 50.1183 30 ATCAAAC 15 0.008246787 50.1183 31 CTGTCCT 15 0.008246787 50.1183 34 TGGCTGT 15 0.008246787 50.1183 31 TCAAACT 15 0.008320027 50.00668 32 CAAACTC 15 0.008320027 50.00668 33 ATCCTTG 15 0.0083937505 49.895554 24 ATGGGTT 30 1.0487347E-6 49.674778 5 >>END_MODULE