FastQCFastQC Report
Fri 17 Jun 2016
SRR1526878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16972
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGATTTTTCTTTATTGCCAATCTTTGAAGTCATTCAACCCAGGACTGAAAGAGGCTC620.36530756540183834No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.3299552203629507No Hit
GGCTGCGGGAGGTCTACCTGGTAGATTTTCTTTAAATGGGGGACACCTGTAATGGGTTTTTATA490.2887108178175819No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.21800612773980674No Hit
GGCTGCGGGCACCCACTCCCCCTAAAAAAATTATTTATTTATTATATCTAAGTACACTGAAGCT370.21800612773980674No Hit
GGCTGCGGGATGCTTTGCCCTCCTTTGTAATTTGTAAAAGTCACATTCTTGACTTTCTCCTTGG220.1296252651425878No Hit
GGCTGCGGGGGTGATCCGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGAT220.1296252651425878No Hit
GGCTGCGGGATCCACACATTGTCCCACACACCCCTGGTTAGCCATCTCAATACCTAATTACCTG210.12373320763610653No Hit
GGCTGCCAGTTACACTGCGATTGTAACAACATTTTTACTTTCTGTACAACAAGGAAAAGCTTTG210.12373320763610653No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11784115012962526No Hit
GGCTGCGGCAGTGCAGGATTGCAAATGGTTTAATGTAATCAAGAAATTCAAGCTGAAGAGATGT180.10605703511666273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAG150.00438240258.7365356
CTGAAGC150.00438240258.7365357
AAACTGG150.00459487858.04142439
AAATTCC150.00459487858.04142428
AATTCCG150.00459487858.04142429
TTATTAT150.00459487858.04142439
GTACACT150.00459487858.04142452
TACACTG201.9470953E-458.04142453
AGTACAC150.00459487858.04142451
ATTATAT150.00459487858.04142441
GCAAACT150.00459487858.04142437
TATTATA150.00459487858.04142440
ATTCCGG150.00459487858.04142430
GCCCGCG150.00459487858.04142431
TTTATTT150.00459487858.04142434
CAAACTG150.00459487858.04142438
GCGGGAG1850.057.870215
GAGGCAA150.00464917457.870219
GGCGCTT150.00464917457.8702120
CCTGTGG150.00464917457.8702125
Produced by FastQC (version 0.11.5)