FastQCFastQC Report
Fri 17 Jun 2016
SRR1526877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26899
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1610.598535261533886No Hit
GGCTGCGGGATTTTTCTTTATTGCCAATCTTTGAAGTCATTCAACCCAGGACTGAAAGAGGCTC1060.3940666939291423No Hit
GGCTGCGGGAGGTCTACCTGGTAGATTTTCTTTAAATGGGGGACACCTGTAATGGGTTTTTATA990.3680434216885386No Hit
GGCTGCGGGATCCACACATTGTCCCACACACCCCTGGTTAGCCATCTCAATACCTAATTACCTG910.33830253912784863No Hit
GGCTGCGGGATTCAAGCCACTACACATGTTGGCTGATGTTCCTTTTAATTCCTGTGCCCTGAAA870.32343209784750365No Hit
GGCTGCGGGATAACTGGGGGCCGGTTAGCAAGAGCCTCCAGACAGTGCTAGGCAGTGCTTTGTG830.3085616565671586No Hit
GGCTGCGGGCACCCACTCCCCCTAAAAAAATTATTTATTTATTATATCTAAGTACACTGAAGCT820.3048440462470724No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.24164467080560617No Hit
GGCTGCGGGCTGTTCCGTTTTGGTTGATGGGTGTTTGGGAGGGGGCTGCGGGGGATACTTTTTT630.23420945016543365No Hit
GGCTGCGGGAGGAGGAGTACATCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTA600.22305661920517494No Hit
GGCTGCGGGGTGCTGGCTGCGGGGTGCCTGTACCAGAGTTTGCGGACAGCGATCCTGCCAACAT580.2156213985650024No Hit
GGCTGCGGGAACCATACCTGGCTTTGCCCTTGGTCTCTCTTAGGCGGACTCAGTTCTTCTTCAG570.21190378824491618No Hit
GGCTGCGGCAGTGCAGGATTGCAAATGGTTTAATGTAATCAAGAAATTCAAGCTGAAGAGATGT570.21190378824491618No Hit
GGCTGCGGGATTCCATTCCCTGCCCTCCCATACACCCTCTGTCTGTTCCACATCCCATATCTCA570.21190378824491618No Hit
GGCTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA560.2081861779248299No Hit
GGCTGCGGGATCGCAGAGATGGTTCGGCACGCCCTGGATGCCCACCAGAGGTCCATTCCAGCTG540.20075095728465742No Hit
GGCTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG490.18216290568422616No Hit
GGCTGCGGGATCCGGACCCAGTCTTCTCTCTATTGCTCCCTCCCCGGGGGTCACCGGCACTGCC440.16357485408379493No Hit
GGCTGCGGGGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGAGCACTCTG420.15613963344362244No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1524220231235362No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC410.1524220231235362No Hit
GGCTGCGGGGCTTCTGTTTGCCCTTTACACTGTCCAAGTAAGTAAATAACTGCCCAATTATAAA400.14870441280344993No Hit
GGCTGCGGGACCACAGCCACGTTTCAGACCTTCTCCCCCGATACCTTCACAGTCATCCTGGACT390.14498680248336368No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT390.14498680248336368No Hit
GGCTGCGGGATTGCGAGCTGGGCACGGAGCAGGCGCCATGGTTCTGCTGCACGTGCTGTTCGAG370.1375515818431912No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC360.13383397152310494No Hit
GGCTGCGGGTGAGAAAAAGCTAAGTTTTGTCATAGGTTGATCTTCACTAATTTGCTAGCCAGAA360.13383397152310494No Hit
GGCTGCGGGGGAAAGTCATTCTGAATGAGAAGTAAATTATTTTGTGTAATACATCCTGAGTGTG350.1301163612030187No Hit
GGCTGCGGGATTGTGTCTCTTCTTTTATGTGGTACTCAACTGCTCTGTAAGAAAGTAACACTCA350.1301163612030187No Hit
GGCTGCGGGCAATCTTGATCTGAAACAATACGCATTGCAGTTCCTCAGCCTAAGAACACAGAAC340.12639875088293245No Hit
GGCTGCGGGGGGGTTGGAGAGATGGCTCAGCGGGTAAGAACACTGACTGCTCTTCCAGGGGTCC330.1226811405628462No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11896353024275995No Hit
GGCTGCGGGATCTACGTGGCTAATGGGGACCTGTTCGGACTGATGGACGAGGAGGATGACGGCA310.1152459199226737No Hit
GGCTGCGGGGAAGGAGCCATTATATCACAGTAATGTCCGACCCATAATCTCTTTTCCCTCATTA310.1152459199226737No Hit
GGCTGCGGGGTGTCGTGCGAGCGCCGGGAACGAGCACTGCCTGGGCAGAACACAGCGCACAGGA310.1152459199226737No Hit
GGCTGCGGGATGCTTTGCCCTCCTTTGTAATTTGTAAAAGTCACATTCTTGACTTTCTCCTTGG300.11152830960258747No Hit
GGCTGCGGGGGTGATCCGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGAT300.11152830960258747No Hit
GGCTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT290.1078106992825012No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC290.1078106992825012No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC280.10409308896241495No Hit
GGCTGCGGGGGAGTAGAACAAGCCCCAGCTCAGGTGGCATGGTTGGCTCAGACCCATGCCCCTG280.10409308896241495No Hit
GGCTGCGGGGAGGAGGACAAATTCTACCTTGAACCCTATCTAAAAGAGGTTATTCGGGAAAGAA280.10409308896241495No Hit
GGCTGCGGGACTCTAGATGTTAAAGAGGTTGCCAGTGTATGACAAAGTAGAGTTAGTAGAACAA270.10037547864232871No Hit
GGCTGCGGGCATGCAACGTAATGCCTCAGTGCCCAACCTTCGTGGCTCTGAGGAGAGGCTCCTG270.10037547864232871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT201.9662236E-458.05224252
GGGTAGA201.9662236E-458.05224240
TTTTTAT201.9662236E-458.05224257
GTTTTTA201.9662236E-458.05224256
ACCTGTA201.9662236E-458.05224245
CGGGTAG201.9662236E-458.05224239
AATGGGT201.9662236E-458.05224251
TGGGTTT201.9662236E-458.05224253
CCGGGTA201.9662236E-458.05224238
GTAATGG201.9662236E-458.05224249
GGAGTTC201.9662236E-458.0522428
CTGTAAT201.9662236E-458.05224247
TGTAATG201.9662236E-458.05224248
CGGGGTC351.5506885E-858.052246
CGGGATG550.058.052246
CGGGACT700.058.052246
GGGATTC351.5506885E-858.052247
ACAGTGC150.00462103958.05223542
ACCGGCT150.00462103958.05223544
ACCGGCA150.00462103958.05223553
Produced by FastQC (version 0.11.5)