FastQCFastQC Report
Fri 17 Jun 2016
SRR1526876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26798
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1270.47391596387790136No Hit
GGCTGCGGGATTTTTCTTTATTGCCAATCTTTGAAGTCATTCAACCCAGGACTGAAAGAGGCTC1050.3918202850959027No Hit
GGCTGCGGGATCCACACATTGTCCCACACACCCCTGGTTAGCCATCTCAATACCTAATTACCTG950.3545040674677215No Hit
GGCTGCGGGCACCCACTCCCCCTAAAAAAATTATTTATTTATTATATCTAAGTACACTGAAGCT820.3059929845510859No Hit
GGCTGCGGGATTCAAGCCACTACACATGTTGGCTGATGTTCCTTTTAATTCCTGTGCCCTGAAA790.2947981192626315No Hit
GGCTGCGGGAGGTCTACCTGGTAGATTTTCTTTAAATGGGGGACACCTGTAATGGGTTTTTATA770.28733487573699534No Hit
GGCTGCGGGATAACTGGGGGCCGGTTAGCAAGAGCCTCCAGACAGTGCTAGGCAGTGCTTTGTG650.24255541458317784No Hit
GGCTGCGGGCTGTTCCGTTTTGGTTGATGGGTGTTTGGGAGGGGGCTGCGGGGGATACTTTTTT570.21270244048063291No Hit
GGCTGCGGGAGGAGGAGTACATCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAAGGAAAGTA570.21270244048063291No Hit
GGCTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG570.21270244048063291No Hit
GGCTGCGGCAGTGCAGGATTGCAAATGGTTTAATGTAATCAAGAAATTCAAGCTGAAGAGATGT560.20897081871781475No Hit
GGCTGCGGGATCCGGACCCAGTCTTCTCTCTATTGCTCCCTCCCCGGGGGTCACCGGCACTGCC530.1977759534293604No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.18658108814090604No Hit
GGCTGCGGGAACCATACCTGGCTTTGCCCTTGGTCTCTCTTAGGCGGACTCAGTTCTTCTTCAG480.1791178446152698No Hit
GGCTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA480.1791178446152698No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.17538622285245167No Hit
GGCTGCGGGATTCCATTCCCTGCCCTCCCATACACCCTCTGTCTGTTCCACATCCCATATCTCA460.17165460108963354No Hit
GGCTGCGGGATCGCAGAGATGGTTCGGCACGCCCTGGATGCCCACCAGAGGTCCATTCCAGCTG450.16792297932681544No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC430.1604597358011792No Hit
GGCTGCGGGGTGCTGGCTGCGGGGTGCCTGTACCAGAGTTTGCGGACAGCGATCCTGCCAACAT430.1604597358011792No Hit
GGCTGCGGGATGCTTTGCCCTCCTTTGTAATTTGTAAAAGTCACATTCTTGACTTTCTCCTTGG400.14926487051272483No Hit
GGCTGCGGGATTGCGAGCTGGGCACGGAGCAGGCGCCATGGTTCTGCTGCACGTGCTGTTCGAG400.14926487051272483No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC390.1455332487499067No Hit
GGCTGCGGGGGAGTAGAACAAGCCCCAGCTCAGGTGGCATGGTTGGCTCAGACCCATGCCCCTG380.1418016269870886No Hit
GGCTGCGGGGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGAGCACTCTG370.13807000522427046No Hit
GGCTGCGGGGGTGATCCGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGAT370.13807000522427046No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC370.13807000522427046No Hit
GGCTGCGGGGCTTCTGTTTGCCCTTTACACTGTCCAAGTAAGTAAATAACTGCCCAATTATAAA360.13433838346145233No Hit
GGCTGCGGGGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCAAGAGACA350.13060676169863422No Hit
GGCTGCGGGACCACAGCCACGTTTCAGACCTTCTCCCCCGATACCTTCACAGTCATCCTGGACT320.11941189641017987No Hit
GGCTGCGGGATTGTGTCTCTTCTTTTATGTGGTACTCAACTGCTCTGTAAGAAAGTAACACTCA310.11568027464736175No Hit
GGCTGCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG300.11194865288454363No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT300.11194865288454363No Hit
GGCTGCGGGAATGATCACGTTAGAATTCACACTGGAATAAAATCATACAAATGTATGGAATGTG290.1082170311217255No Hit
GGCTGCGGGGTGTCGTGCGAGCGCCGGGAACGAGCACTGCCTGGGCAGAACACAGCGCACAGGA290.1082170311217255No Hit
GGCTGCGGGAAGAAACCCAGCGACATTCCCGCGTGGAGGTCCTAATGGAGTCTGTCCAGAATAG280.10448540935890738No Hit
GGCTGCGGAGACAAATTTTACTTCCCACAATCCTCCTGCATCCAGTCTATAAAAATGTTGAGTA280.10448540935890738No Hit
GGCTGCGGGGGAAAGTCATTCTGAATGAGAAGTAAATTATTTTGTGTAATACATCCTGAGTGTG280.10448540935890738No Hit
GGCTGCGGGGACTGTTCACTTCTGTAAAACCTCGAGGTTTAATGGTCATTCTGCATTCCTTAAA270.10075378759608926No Hit
GGCTGCGGGCAATCTTGATCTGAAACAATACGCATTGCAGTTCCTCAGCCTAAGAACACAGAAC270.10075378759608926No Hit
GGCTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT270.10075378759608926No Hit
GGCTGCGGGGGCCTGGGCAGAACACAGCGCACAGGAGCCAGCTTGCGGCGCGCCCTGAAGAGGT270.10075378759608926No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.10075378759608926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTTT201.8915106E-458.50568426
AGTGCTA258.015082E-658.50568444
GTGCTAG258.015082E-658.50568445
GATGGGT150.004480115658.5056826
GCCTAGC150.004480115658.5056844
TATCTAA150.004480115658.5056845
AAAAATT150.004480115658.5056826
ATATCTA150.004480115658.5056844
TGATGGG150.004513899358.39508425
ATCCCCA258.10591E-658.39508424
CCAATCT201.9093536E-458.39508424
TCAAGCG150.004513899358.39508422
TATATCT150.004513899358.39508443
ACTTGAC201.9093536E-458.39508443
CCTAAAA150.004513899358.39508421
AACCCAG201.9093536E-458.39508443
TTATATC150.004513899358.39508442
TGCCTAG150.004513899358.39508443
GCCGGTT258.10591E-658.39508420
AAGATCC258.10591E-658.39508421
Produced by FastQC (version 0.11.5)