FastQCFastQC Report
Fri 17 Jun 2016
SRR1526875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25059
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG1350.5387286005028133No Hit
GGCTGCGGGATTTTTCTTTATTGCCAATCTTTGAAGTCATTCAACCCAGGACTGAA980.39107705814278304No Hit
GGCTGCGGGATTCAAGCCACTACACATGTTGGCTGATGTTCCTTTTAATTCCTGTG930.3711241470130492No Hit
GGCTGCGGGATCCACACATTGTCCCACACACCCCTGGTTAGCCATCTCAATACCTA920.36713356478710246No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC850.3391994892054751No Hit
GGCTGCGGGAACCATACCTGGCTTTGCCCTTGGTCTCTCTTAGGCGGACTCAGTTC830.33121832475358154No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC790.31525599584979447No Hit
GGCTGCGGGATTCCATTCCCTGCCCTCCCATACACCCTCTGTCTGTTCCACATCCC670.2673690091384333No Hit
GGCTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC670.2673690091384333No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT670.2673690091384333No Hit
GGCTGCGGGAGGTCTACCTGGTAGATTTTCTTTAAATGGGGGACACCTGTAATGGG650.25938784468653975No Hit
GGCTGCGGCAGTGCAGGATTGCAAATGGTTTAATGTAATCAAGAAATTCAAGCTGA600.23943493355680595No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT570.22746318687896566No Hit
GGCTGCGGGTGAGAAAAAGCTAAGTTTTGTCATAGGTTGATCTTCACTAATTTGCT520.2075102757492318No Hit
GGCTGCGGGGTGCTGGCTGCGGGGTGCCTGTACCAGAGTTTGCGGACAGCGATCCT520.2075102757492318No Hit
GGCTGCGGGAGGAGGAGTACATCAAGCGCGCCCTGGCCAACAGCCTCGCTTGTCAA500.1995291112973383No Hit
GGCTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT500.1995291112973383No Hit
GGCTGCGGGATCGCAGAGATGGTTCGGCACGCCCTGGATGCCCACCAGAGGTCCAT500.1995291112973383No Hit
GGCTGCGGGATAACTGGGGGCCGGTTAGCAAGAGCCTCCAGACAGTGCTAGGCAGT490.19553852907139152No Hit
GGCTGCGGGATGCTTTGCCCTCCTTTGTAATTTGTAAAAGTCACATTCTTGACTTT470.18755736461949798No Hit
GGCTGCGGGCACCCACTCCCCCTAAAAAAATTATTTATTTATTATATCTAAGTACA450.17957620016760445No Hit
GGCTGCGGGGGAAAGTCATTCTGAATGAGAAGTAAATTATTTTGTGTAATACATCC440.1755856179416577No Hit
GGCTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT410.16361387126381738No Hit
GGCTGCGGGCAATCTTGATCTGAAACAATACGCATTGCAGTTCCTCAGCCTAAGAA400.15962328903787062No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.15962328903787062No Hit
GGCTGCGGGAACATTTATCTATCTACTTTCCTCTCATGATTTTTTTTAAATTTTAT380.1516421245859771No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC380.1516421245859771No Hit
GGCTGCGGGAGCTTTGGCCACCAAGCCAGGCATCAATTGCCCTCCCCTCTGCCCTG360.14366096013408355No Hit
GGCTGCGGAGACAAATTTTACTTCCCACAATCCTCCTGCATCCAGTCTATAAAAAT360.14366096013408355No Hit
GGCTGCGGGGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGA330.13168921345624326No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA330.13168921345624326No Hit
GGCTGCGGGATCCGGACCCAGTCTTCTCTCTATTGCTCCCTCCCCGGGGGTCACCG330.13168921345624326No Hit
GGCTGCGGGACTCTAGATGTTAAAGAGGTTGCCAGTGTATGACAAAGTAGAGTTAG330.13168921345624326No Hit
GGCTGCGGGGAGATGCTTTAGCTCTGCAGTGGAGTCCCGCTGTATCTGACCATCCG320.1276986312302965No Hit
GGCTGCGGGACCACAGCCACGTTTCAGACCTTCTCCCCCGATACCTTCACAGTCAT320.1276986312302965No Hit
GGCTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG310.12370804900434973No Hit
GGCTGCGGGATTGCGAGCTGGGCACGGAGCAGGCGCCATGGTTCTGCTGCACGTGC310.12370804900434973No Hit
GGCTGCGGGGCTTCTGTTTGCCCTTTACACTGTCCAAGTAAGTAAATAACTGCCCA300.11971746677840298No Hit
GGCTGCGGGGTGTCGTGCGAGCGCCGGGAACGAGCACTGCCTGGGCAGAACACAGC300.11971746677840298No Hit
GGCTGCGGGGAGTTCCCAGGCGCTTGGGGGCCCGCGCTGGCCTGGGAGGAGTAGCC280.11173630232650944No Hit
GGCTGCGGGGAAGGAGCCATTATATCACAGTAATGTCCGACCCATAATCTCTTTTC280.11173630232650944No Hit
GGCTGCGGGAAGACCAAGCGTACCATCCAGTTTGTGGACTGGTGCCCCACTGGCTT270.10774572010056266No Hit
GGCTGCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAA270.10774572010056266No Hit
GGCTGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCACTTCC260.1037551378746159No Hit
GGCTGCGGGCTGTTCCGTTTTGGTTGATGGGTGTTTGGGAGGGGGCTGCGGGGGAT260.1037551378746159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATG150.00784773250.76578550
GAGTTAG150.00784773250.76578550
TGCCCAT150.00784773250.76578549
ATAAACA150.00784773250.76578550
TAGAGTT150.00784773250.76578548
ACAGCGA251.9450386E-550.35555646
TCGTCTG203.9634004E-450.35555644
ATCGTCT203.9634004E-450.35555643
CAGCGAT251.9450386E-550.35555647
CATCGTC203.9634004E-450.35555642
GTAGAGT150.00810429450.35555347
TAATTCC150.00810429450.35555346
CAAAGTA150.00810429450.35555343
TTAATTC150.00810429450.35555345
TTCATCG204.0032263E-450.25403240
ATGACAA150.00816939750.25403239
TGACAAA150.00816939750.25403240
GACAAAG150.00816939750.25403241
TCATCGT204.0032263E-450.25403241
TTTGCGG251.9685176E-550.25403239