FastQCFastQC Report
Fri 17 Jun 2016
SRR1526874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159060
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT147419.267571985414309No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT7480.4702627939142462No Hit
TAGCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT4530.28479818936250473No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT4470.2810260279139947No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT3130.19678108889727147No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.16911857160819818No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.15277253866465484No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC2390.15025776436564817No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAG2380.14962907079089652No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTACGCCATGCAT2180.1370551992958632No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAGCCATGCAT2000.1257387149503332No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1890.11882308562806487No Hit
TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG1850.11630831132905821No Hit
TAGCATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA1740.10939268200678988No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAGCCATGCAT1680.1056205205582799No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.10121966553501822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA35800.058.0712935
ATAGGGG150.00465709158.0712935
CATGAGG150.00465709158.0712934
AACGATT650.058.03468347
TTAGGTG258.604795E-658.0346855
ACGATTA550.058.0346848
GATTAAC453.092282E-1158.0346850
GTACGCT258.604795E-658.0346849
ATATACC150.00466873958.0346749
AGGATTA150.00466873958.0346748
TATACCT150.00466873958.0346750
TATTAGT150.004674571658.01638852
TAAGCCC1350.058.01638853
CTTAGGT258.620897E-658.01638454
TGTGTCA258.637024E-657.99810841
AATCTCA150.00468040957.99810443
TGTAGTA150.00468040957.99810443
TAGGCTC150.00468040957.99810441
TCTCAAC600.057.99810443
TATGTCT150.00468040957.99810442
Produced by FastQC (version 0.11.5)