FastQCFastQC Report
Fri 17 Jun 2016
SRR1526873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222174
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3662216.483476914490446No Hit
TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG15140.681447874188699No Hit
TAGCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT14240.6409390837811805No Hit
TAGCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4850.21829737052940487No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4720.21244610080387444No Hit
TAGCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4500.20254395203759215No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4490.20209385436639754No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4430.19939326833922963No Hit
TAGCATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG4310.19399209628489383No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.19354199861369917No Hit
TAGCATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA4030.18138936149144363No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3780.1701369197115774No Hit
TAGCATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC3720.16743633368440952No Hit
TAGCATGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG3680.16563594299963091No Hit
TAGCATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT3590.1615850639588791No Hit
TAGCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG3480.15663398957573793No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3450.155283696562154No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.14943242683662356No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3320.14943242683662356No Hit
TAGCATGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA3190.1435811571110931No Hit
TAGCATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3120.14043047341273057No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2920.13142851998883756No Hit
TAGCATGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC2820.12692754327689107No Hit
TAGCATGGGGGTTGGCCATAGTAAGGAACAGAAACTCGGCTGCTGGGGAATGGCATTCTTTAAA2800.12602734793450177No Hit
TAGCATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2670.12017607820897133No Hit
TAGCATGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA2630.11837568752419275No Hit
TAGCATGGGGAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCCAACTCTTTC2610.11747549218180345No Hit
TAGCATGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT2360.10622305040193722No Hit
TAGCATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC2280.10262226903238003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT150.004642348758.1254854
TAGGCCG150.004642348758.1254853
TAAGACA150.004642348758.1254852
ATACCTT150.004642348758.1254851
ACGCTTA201.9854849E-458.12547751
CCTTTGT303.697951E-758.11234348
GATTAGG150.004646506658.11234350
TCGGGCA201.9877058E-458.1123456
AACGATT150.004650667358.0992147
TACCATT150.00465483158.08608241
GAACTAC150.00465483158.08608242
ATTAATT150.00465483158.08608241
ACCGTAC150.00465483158.08608244
ACCCGAT150.00465483158.08608241
CACCGTA150.00465483158.08608243
TAGGATT550.058.03363834
TTAATGC150.004671512658.03363433
CAAATCG150.004671512658.03363432
GTGCAAA150.0046756958.0205429
CGTTAAA150.0046756958.0205431
Produced by FastQC (version 0.11.5)