FastQCFastQC Report
Fri 17 Jun 2016
SRR1526872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223083
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3478715.593747618599355No Hit
TAGCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT13000.5827427459734718No Hit
TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG12840.5755705275614906No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4450.19947732458322687No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.1923051061712457No Hit
TAGCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4240.19006378791750156No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4150.18602941506076215No Hit
TAGCATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG4110.18423636045776684No Hit
TAGCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG3930.176167614744288No Hit
TAGCATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA3880.1739262964905439No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3830.17168497823679976No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3550.15913359601583266No Hit
TAGCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG3480.1559957504605909No Hit
TAGCATGGGTACCTGGTTGATCCTGNCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3330.14927179569935853No Hit
TAGCATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT3330.14927179569935853No Hit
TAGCATGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG3320.1488235320486097No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.14434089554112148No Hit
TAGCATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2960.13268604062165204No Hit
TAGCATGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2920.13089298601865673No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2910.1304447223679079No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2880.12909993141566142No Hit
TAGCATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2870.12865166776491263No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2820.12641034951116847No Hit
TAGCATGGGGGTTGGCCATAGTAAGGAACAGAAACTCGGCTGCTGGGGAATGGCATTCTTTAAA2670.11968639474993611No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCNCAAAGATTAAGCCATGCAT2570.11520375824244787No Hit
TAGCATGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC2490.1116176490364573No Hit
TAGCATGGGGAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCCAACTCTTTC2450.10982459443346199No Hit
TAGCATGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA2340.10489369427522492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATC201.9274134E-458.47546845
CAATTAT201.9295797E-458.4621944
TAATTAC150.004545538758.43564242
CCTTGAT201.9339181E-458.43564242
GCTAATT150.004553717658.40912240
CTAATTA150.004553717658.40912241
TTCGGTA150.004574212758.34292625
GATACAT201.953537E-458.31649422
ATAGAAC201.953537E-458.31649423
ATACATC201.953537E-458.31649423
TCATCTA150.0045824358.3164923
AGATACA201.9601117E-458.27688621
CCAAATA150.004594776358.27688621
TAGATAC201.9623071E-458.2636920
CAAGCAT150.004652721358.09275458
CGCGACA150.00465688158.07964756
CCTAGTC201.9977015E-458.05345553
ATTATCT201.9977015E-458.05345546
TTATGAT150.00466520958.0534553
CACTATC150.00466520958.0534547
Produced by FastQC (version 0.11.5)