FastQCFastQC Report
Fri 17 Jun 2016
SRR1526871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209651
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3935318.770718956742396No Hit
TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC12390.5909821560593558No Hit
TAGCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC11900.5676099803959914No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6450.3076541490381634No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA5770.2752192930155353Illumina Single End Adapter 1 (95% over 21bp)
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4740.22609002580478987No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4690.22370510992077308No Hit
TAGCATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA4460.21273449685429596No Hit
TAGCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC4140.19747103519658862No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4030.19222422025175173No Hit
TAGCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA3850.18363852306929135No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3560.16980601094199407No Hit
TAGCATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3420.16312824646674712No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA3290.1569274651683035No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG3250.1550195324610901No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.1550195324610901No Hit
TAGCATGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC3180.15168065022346663No Hit
TAGCATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3010.1435719362178096No Hit
TAGCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA2980.14214098668739952No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2770.1321243399745291No Hit
TAGCATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2720.1297394240905123No Hit
TAGCATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2660.1268775250296922No Hit
TAGCATGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2500.11924579420083854No Hit
TAGCATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG2440.1163838951400184No Hit
TAGCATGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC2290.1092291474879681No Hit
TAGCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2220.10589026525034463No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGC2170.10350534936632784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG203.8729643E-450.76880350
GTACGCT203.8729643E-450.76880349
CTATCTC150.00792832550.768849
ATGAACC150.00792832550.768849
CTTAGAT150.00792832550.768849
CTAACTT150.008183097550.36431547
TCCTTAT150.008183097550.36431547
ATCCTTA150.00819091250.3521646
ACCCTAT150.00819091250.3521646
ACATTTG251.9968564E-550.35215846
TTAAGCA507.2759576E-1250.3400145
ATGCCCT150.008206557550.32786644
GACCACT204.0530268E-450.30359642
TCTCAAC150.00822222450.30359343
TATCCCC150.00829300350.19465639
GAGATCA150.00830879350.17051738
GTACATC150.0083325250.13434235
ACAGAAC150.0083325250.13434236
ACGCACA252.0575124E-550.09822534
ATATGCC750.050.09822534
Produced by FastQC (version 0.11.5)