##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526871_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 209651 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.64610710180252 39.0 39.0 40.0 36.0 40.0 2 38.4190964984665 39.0 38.0 40.0 35.0 40.0 3 38.326084778989845 39.0 38.0 40.0 35.0 40.0 4 37.91255944402841 39.0 38.0 40.0 34.0 40.0 5 38.20754491989067 39.0 38.0 40.0 35.0 40.0 6 38.464586384038235 39.0 38.0 40.0 35.0 40.0 7 38.65941493243533 39.0 38.0 40.0 37.0 40.0 8 38.677087159135894 39.0 39.0 40.0 37.0 40.0 9 38.693991442921806 39.0 39.0 40.0 37.0 40.0 10 38.198844746745785 39.0 38.0 40.0 35.0 40.0 11 38.166085542162925 39.0 38.0 40.0 35.0 40.0 12 38.053002370606386 39.0 38.0 40.0 35.0 40.0 13 37.923267716347645 39.0 38.0 40.0 35.0 40.0 14 38.06888591039394 39.0 38.0 40.0 35.0 40.0 15 37.95524466852054 39.0 38.0 40.0 35.0 40.0 16 37.90655422583246 39.0 38.0 40.0 35.0 40.0 17 37.77779738708616 39.0 38.0 40.0 34.0 40.0 18 37.815321653605274 39.0 38.0 40.0 34.0 40.0 19 37.55251823268193 39.0 38.0 40.0 33.0 40.0 20 37.50403766259164 39.0 37.0 40.0 33.0 40.0 21 37.464276344973314 39.0 38.0 40.0 33.0 40.0 22 37.45736008890967 39.0 38.0 40.0 33.0 40.0 23 37.41395938965233 39.0 37.0 40.0 33.0 40.0 24 37.153574273435375 39.0 37.0 40.0 33.0 40.0 25 36.927541485611805 39.0 36.0 40.0 32.0 40.0 26 36.756099422373374 39.0 36.0 40.0 32.0 40.0 27 36.64152329347344 39.0 36.0 40.0 31.0 40.0 28 36.053627218568 38.0 35.0 40.0 30.0 40.0 29 36.16867556081297 38.0 35.0 40.0 30.0 40.0 30 35.69078611597369 38.0 35.0 40.0 29.0 40.0 31 35.224225021583486 38.0 35.0 39.0 28.0 40.0 32 35.1338748682334 38.0 35.0 39.0 28.0 40.0 33 34.886859590462244 38.0 34.0 39.0 27.0 40.0 34 35.12101540178678 38.0 35.0 40.0 28.0 40.0 35 34.82886320599472 38.0 34.0 40.0 27.0 40.0 36 34.661589975721554 38.0 34.0 40.0 27.0 40.0 37 34.33590586260023 38.0 33.0 39.0 25.0 40.0 38 34.3885075673381 38.0 34.0 40.0 25.0 40.0 39 34.23692231374999 38.0 33.0 40.0 24.0 40.0 40 33.83720087192525 38.0 33.0 40.0 23.0 40.0 41 33.50616977739195 38.0 33.0 39.0 19.0 40.0 42 33.23372652646541 38.0 33.0 39.0 18.0 40.0 43 32.840949959694925 37.0 32.0 39.0 16.0 40.0 44 32.94100672069296 38.0 33.0 39.0 14.0 40.0 45 32.45659691582678 37.0 32.0 39.0 6.0 40.0 46 32.11932211150913 37.0 32.0 39.0 4.0 40.0 47 31.598504180757544 36.0 31.0 39.0 4.0 40.0 48 31.497746254489606 36.0 31.0 39.0 4.0 40.0 49 31.134332772083127 36.0 30.0 39.0 4.0 40.0 50 30.870265345741256 36.0 30.0 39.0 4.0 40.0 51 29.770132267434928 35.0 29.0 39.0 4.0 40.0 52 29.678723211432334 35.0 28.0 39.0 4.0 40.0 53 29.480493772984627 35.0 28.0 39.0 4.0 40.0 54 28.99211546808744 35.0 27.0 39.0 4.0 40.0 55 28.71140132887513 35.0 26.0 39.0 4.0 40.0 56 17.457760754778178 21.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.337877410535512 1 2 -0.6581774431701106 1 3 -0.6581295589849958 1 4 -0.8144058702965964 1 5 -1.2306051922374337 1 6 -1.0249794430209747 1 7 -0.5529990573263817 1 8 -0.6130883742523423 1 9 -0.6716392971952203 1 10 -0.7025614931965194 1 11 -0.6728400647805728 1 12 -0.9301845227519294 1 13 -0.5380939331629335 1 14 -0.6527134310780554 1 15 -0.6367335193210124 1 16 -0.7162720123681225 1 17 -0.33203302541691926 1 18 -0.5150699108865311 1 19 0.016271657542127116 1 20 -0.43893746737518313 1 21 -0.2555706929744801 1 22 -0.581636608647095 1 23 -0.105820070125489 1 24 -0.371946120980887 1 25 -0.6523438121056415 1 26 -0.08310386001239323 1 27 -0.7391226228190533 1 28 0.11315934184288068 1 29 0.2009923743952342 1 30 -0.25232725288179836 1 31 -0.26537878905385526 1 32 0.6525372924804742 1 33 0.3967026432466554 1 34 -0.3960495865537865 1 35 -1.360234185344936 1 36 0.3891539786458722 1 37 0.7824338443367438 1 38 0.870123496542341 1 39 0.575338934798701 1 40 0.928299420281725 1 41 1.2988980748143177 1 42 0.873534638923843 1 43 1.3894129667303972 1 44 1.3986898006110664 1 45 1.6237481157065332 1 46 2.079050872534072 1 47 1.9560741809374917 1 48 1.53511454197686 1 49 1.2689526432651554 1 50 1.5423010049789063 1 51 1.4728688667168477 1 52 1.170476936356934 1 53 0.9174113939002595 1 54 1.0761929897385727 1 55 0.9768710454480569 1 56 1.7151398394778106 2 1 -0.3020981503778444 2 2 -0.4221115078925308 2 3 -0.5109880407094565 2 4 -0.5593752732405335 2 5 -0.6818979867685542 2 6 -0.4319754185433311 2 7 -0.2521555709158889 2 8 -0.2593478152358628 2 9 -0.12028585985027718 2 10 -0.2619363209157939 2 11 -0.5559923885029363 2 12 -0.08887574056988967 2 13 -0.1909689538366237 2 14 -0.09521301760432266 2 15 -0.21970391460189376 2 16 -0.4492506771236222 2 17 -0.316927451791031 2 18 -0.48483119871937674 2 19 0.01643704703437976 2 20 -0.053497255676631994 2 21 -0.25085709244523713 2 22 -0.2873811370113941 2 23 -0.12674184089565443 2 24 -0.2718303483363087 2 25 -0.5270061418922936 2 26 -0.9208843330263434 2 27 -0.8032386159829557 2 28 -0.6959811840957073 2 29 -1.0524670224844215 2 30 -0.8536558818029079 2 31 -0.9533990768315661 2 32 -0.837622032730394 2 33 -0.9657208641131803 2 34 -0.9707505072219575 2 35 -0.27084116478151543 2 36 -0.730698273407711 2 37 -0.4113199458391179 2 38 0.15511715661180148 2 39 -0.031061638551541648 2 40 -0.43448243097799377 2 41 0.2658201763634622 2 42 -0.03175231184521721 2 43 0.2599681241260612 2 44 -0.18899419519906502 2 45 -0.5308359297204497 2 46 -0.27965137125003636 2 47 -0.3328888269460748 2 48 -0.5650287955830926 2 49 -0.8252366725736415 2 50 -0.6660621903460857 2 51 0.3317640649085867 2 52 0.5087260129322679 2 53 -0.030711435362654527 2 54 0.14995670328397281 2 55 0.1801898612592936 2 56 0.4228139119184071 3 1 -0.07587260861474476 3 2 -0.029043971599662655 3 3 -0.07510544024658827 3 4 -0.08314973147743387 3 5 -0.26337284475298617 3 6 -0.1209087238242077 3 7 -0.03166325027628858 3 8 0.07323160828612885 3 9 -0.013721584655662866 3 10 -0.1338067185593914 3 11 -0.6334697712086026 3 12 -0.07819827370685317 3 13 0.14345141224795555 3 14 -0.2070897270509917 3 15 -0.39028039261578584 3 16 0.030972339918243108 3 17 -0.2909438624790184 3 18 -0.04437254102949595 3 19 -0.2597569357133622 3 20 -0.05265568193373582 3 21 -0.6861611109598584 3 22 -0.3908421090290659 3 23 -0.4815703702455423 3 24 -0.07179878932095107 3 25 -0.030740625376402875 3 26 0.24942915319288517 3 27 0.040070872760992415 3 28 0.32616272501432775 3 29 0.19695439556733874 3 30 0.5822793170188874 3 31 0.40642629661677887 3 32 0.35893803459550355 3 33 0.6023940107027315 3 34 0.7683616324792837 3 35 0.7243723845557497 3 36 0.8175344217321978 3 37 0.6728900268097391 3 38 0.824641667447068 3 39 0.764278146847154 3 40 1.05773301190019 3 41 1.5913514197886727 3 42 0.702520778834355 3 43 0.9499007982266292 3 44 1.2900190595873866 3 45 1.4109902853016436 3 46 1.3164458305172708 3 47 1.151752452246015 3 48 1.2546114316418198 3 49 1.0871344614469756 3 50 1.5941944896454991 3 51 1.8173521145614373 3 52 1.4070533851182567 3 53 1.4878031869811288 3 54 1.0694812141192358 3 55 1.1219108795635222 3 56 0.22067841854580195 4 1 -0.1815000080893867 4 2 -0.08961176307268914 4 3 0.03223947791870074 4 4 -0.2655098042194055 4 5 -0.09217664350020982 4 6 -0.01674626676216917 4 7 0.09371561414076268 4 8 0.056207967146505666 4 9 0.02598340625159068 4 10 0.10169540308134373 4 11 -0.079468962964512 4 12 -0.02268200779455043 4 13 0.28024672443223153 4 14 0.09928529820663812 4 15 -0.20352549263598974 4 16 0.058149547434915405 4 17 0.08589994435064341 4 18 -0.36938769560617857 4 19 -0.10007821273902096 4 20 -0.03042896946656981 4 21 -0.34629447123465695 4 22 0.014037664662588156 4 23 0.14956723331073363 4 24 -0.010321723246995873 4 25 0.15156893210995293 4 26 0.7846888016067055 4 27 0.6876259343896081 4 28 0.880062502747208 4 29 0.9997428376768553 4 30 0.8085876621391108 4 31 1.0669132636808314 4 32 1.1529873374849728 4 33 1.031319046063409 4 34 0.8879817577571174 4 35 0.8145421158145894 4 36 1.2535315928778843 4 37 1.1531385618673227 4 38 1.1942617927249017 4 39 1.6795640631938937 4 40 1.8026309710838646 4 41 1.8312483686672323 4 42 1.4864165153467823 4 43 1.9327761265757957 4 44 1.610740417437455 4 45 1.9166479939296437 4 46 1.910151629668615 4 47 2.2263129695222332 4 48 1.8636738684977487 4 49 2.0326790159024206 4 50 1.95295383496779 4 51 1.9936503566305426 4 52 2.4012441307234305 4 53 2.0163948216547993 4 54 1.6940518344465723 4 55 2.1965724273509544 4 56 1.7125454623930416 5 1 -0.12934955494883837 5 2 -0.031122580821126178 5 3 -0.06697996783540106 5 4 -0.13794942913427377 5 5 -0.5579684607771753 5 6 0.0075871189573533115 5 7 -0.024860529187854752 5 8 0.12708648356957042 5 9 0.06375384996263023 5 10 -0.12057920533187882 5 11 0.11577437089268727 5 12 -0.09010303561161948 5 13 0.2568300970552144 5 14 -0.1226226068695837 5 15 -0.17958500334896144 5 16 -0.024961144586669093 5 17 0.2083002796222715 5 18 -0.9997769250053068 5 19 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0.33652808071857976 6 44 0.12798898439941553 6 45 -0.042393173344976276 6 46 0.3536638756300512 6 47 -0.26435925559501783 6 48 0.5439239987739342 6 49 0.39766077723644955 6 50 0.45488192251338333 6 51 0.8930771414142846 6 52 1.0763874345274935 6 53 1.0310899753806986 6 54 1.352832449350167 6 55 0.6741128129684633 6 56 1.0135355614029429 7 1 -0.08377170734053152 7 2 -0.27966554002398425 7 3 -0.1574373577851773 7 4 0.16656538299474732 7 5 -0.12582493218694424 7 6 -0.25454593505387635 7 7 -0.04999951879385378 7 8 0.05932401479187632 7 9 0.018444581303107555 7 10 0.02826724808310388 7 11 0.09487892867744563 7 12 0.09457184957088316 7 13 0.10760763311193244 7 14 -0.21141480733690798 7 15 0.20593321973271372 7 16 -0.42038993087840026 7 17 -0.27814991241167775 7 18 -0.04830914105021833 7 19 -0.3526037190967628 7 20 -0.40503318115677445 7 21 0.3050851337309126 7 22 -0.0712264455047702 7 23 0.10767649601365292 7 24 0.3997903539505714 7 25 0.6146286898623785 7 26 0.2241520372703789 7 27 0.1685024484369535 7 28 0.6137158173172423 7 29 0.4035223019176968 7 30 0.5761154301443412 7 31 0.2967971657766242 7 32 0.6105437530250057 7 33 1.1790800150537066 7 34 0.7185843611646661 7 35 0.8336907311837365 7 36 0.4250398604559109 7 37 0.9427802163881083 7 38 0.32181871085326463 7 39 0.7599530665612377 7 40 0.47521980357117144 7 41 0.5409784787340755 7 42 0.37878722449891455 7 43 0.5967204397888395 7 44 0.19846721042132742 7 45 0.8036661373683529 7 46 0.8942195796108159 7 47 1.0680479044370443 7 48 0.9678352998693143 7 49 1.4932258320053542 7 50 1.3695493016210527 7 51 2.0437584131214734 7 52 2.2148592485803924 7 53 1.9410819604194387 7 54 2.0552369377284734 7 55 2.204890264210782 7 56 1.4307944446516743 8 1 -1.0230565101083045 8 2 -0.9489789847110544 8 3 -1.1213088378067297 8 4 -1.2579872642559096 8 5 -0.7362664714774212 8 6 -0.7882214589741281 8 7 -0.8115059135986016 8 8 -0.8844414050532876 8 9 -1.2244729242976646 8 10 -0.2275816587030448 8 11 -0.18788203890897393 8 12 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2.5211517219258326 8 54 3.1572135137429953 8 55 3.3012518897105956 8 56 3.9487310921292007 9 1 -0.28327710887639057 9 2 -0.22713397683252623 9 3 -0.05346755432298522 9 4 -0.3097850009698462 9 5 -0.28951099231970545 9 6 -0.22692651555627918 9 7 -0.31057481490213945 9 8 -0.5127181562350671 9 9 -0.29237150616272345 9 10 -0.05651734127000907 9 11 0.09737795249626657 9 12 0.07033652482794395 9 13 0.09761153783665577 9 14 0.04856176431479042 9 15 -0.05927568303964392 9 16 0.12798960836408924 9 17 0.04997712129910781 9 18 0.21601469078630942 9 19 0.45455494288423637 9 20 0.4059000480505688 9 21 0.5226824264551055 9 22 0.19395642576947125 9 23 0.4923699751233741 9 24 0.6261342300406412 9 25 0.6557324253823609 9 26 0.6544527010735806 9 27 0.6949374347704165 9 28 0.6356603702262618 9 29 0.936985792741595 9 30 1.27568591042548 9 31 1.2351285467476032 9 32 0.7868030267462061 9 33 1.5428702011789213 9 34 1.387880209140718 9 35 1.6754712856546519 9 36 2.065336619534399 9 37 2.446385578876715 9 38 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0.9596009513129928 120 54 1.785971830466277 120 55 1.4839250319581474 120 56 1.4125305362773162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 83.0 5 7.0 6 14.0 7 38.0 8 63.0 9 49.0 10 73.0 11 118.0 12 174.0 13 216.0 14 312.0 15 414.0 16 644.0 17 738.0 18 1076.0 19 1253.0 20 1299.0 21 1628.0 22 1678.0 23 1663.0 24 2260.0 25 2667.0 26 2839.0 27 3198.0 28 3635.0 29 4045.0 30 4926.0 31 5607.0 32 6972.0 33 8431.0 34 11457.0 35 15291.0 36 21287.0 37 30470.0 38 38340.0 39 36686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.53446441943993 0.35201358448087533 0.09062680359263729 0.022895192486560998 8 99.60636120296016 0.2982112098786638 0.06584503514120897 0.02958255201996345 9 98.87956651768891 0.5490076365006606 0.24803125193774414 0.3233945938726741 10 47.27237170345002 16.99538757268031 27.8014414431603 7.930799280709369 11 30.277461113946515 42.67950069401053 12.496959232247878 14.54607895979509 12 27.010126352843532 12.947231351150245 17.359325736581273 42.68331655942495 13 21.451841393554048 13.03595022203567 20.366227683149614 45.14598070126067 14 21.044974743740788 14.88330606579506 44.61271350959451 19.459005680869637 15 44.241143614864704 15.34120991552628 21.127492833327768 19.29015363628125 16 46.87695264988004 14.661508888581501 18.438261682510458 20.023276779028006 17 24.642381863191684 14.565635270044025 40.632288899170526 20.159693967593764 18 23.17279669546055 15.786712202660613 41.62870675551274 19.411784346366105 19 46.44385192534259 16.770728496405933 17.33213769550348 19.453281882747994 20 24.327095983324668 39.35826683392877 16.74354045532814 19.571096727418425 21 22.041392600082997 17.009697067984412 41.59436396678289 19.3545463651497 22 23.39602482220452 14.573743984049683 19.34452971843683 42.68570147530897 23 23.41462716609985 16.7378166572065 17.763807470510514 42.08374870618313 24 22.88915814379484 16.764616089146696 39.80212944588612 20.54409632117234 25 46.1584336027328 14.952147403173635 17.39344090228147 21.495978091812102 26 22.052246464318614 14.895922331950723 18.72872982764299 44.32310137608767 27 20.36285703375592 14.7892164350084 19.23113639835039 45.61679013288529 28 21.303569296884326 39.118061358481555 18.436194341649756 21.142175002984363 29 45.38121905999732 16.45673655842046 18.343718343240507 19.81832603834171 30 22.956221484662695 15.967632402031583 41.71871562616572 19.35743048714 31 22.01388456956399 37.69837662493003 20.79719055160304 19.490548253902944 32 46.56223385422149 16.015157749686608 18.968239538377624 18.45436885771428 33 24.03746297014161 16.29457342081712 19.789617760931886 39.87834584810938 34 21.769723426864644 38.524464612232784 22.853901605445305 16.85191035545727 35 20.370565473910045 18.813213546854648 44.944352923147655 15.871868056087656 36 21.830375320351607 41.247874302411915 19.43426504754377 17.4874853296927 37 21.496531503909246 18.821861106852676 42.07755250651541 17.60405488272267 38 44.248474711117716 19.033879865228208 19.73745380998711 16.980191613666975 39 23.540891480302275 16.716694157811958 19.25658828493613 40.485826076949635 40 21.36585225418882 17.437575571464215 41.72408498550947 19.472487188837494 41 21.920878466906313 16.481333058172517 41.504009829000346 20.09377864592082 42 41.08517828683346 15.867240154380843 21.236006835768737 21.811574723016953 43 18.85754075923836 15.24335486349557 44.43585372295723 21.463250654308833 44 19.824146061534474 15.155516925101207 22.630509841961956 42.389827171402366 45 19.575336872192732 15.771623471765187 43.87721278793265 20.77582686810943 46 19.214154257185008 16.301710177937313 22.288053741584857 42.19608182329282 47 18.777762821375692 38.05741976424437 23.61882968290291 19.54598773147703 48 20.19402812782577 37.35354098928372 22.653288954729977 19.799141928160534 49 20.235027118513674 37.73945070585067 21.90589298765242 20.11962918798323 50 40.504063397664446 16.759771160221526 22.2844522713166 20.45171317079743 51 20.723606681962263 38.46168645955085 22.43401971111528 18.38068714737161 52 20.49092387408409 17.696160267111853 42.08839901276383 19.72451684604023 53 21.424756662609038 15.776153657398275 43.15022685828935 19.64886282170334 54 21.76523362673068 36.99522320021338 21.10230110812056 20.13724206493538 55 20.206887256553678 37.95355867260982 19.986425731422024 21.853128339414475 56 40.83539076825342 17.300260367787548 19.23545237098319 22.628896492975844 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 2.5 5 5.0 6 17.0 7 29.0 8 87.0 9 145.0 10 222.5 11 300.0 12 302.0 13 286.5 14 269.0 15 360.5 16 452.0 17 564.5 18 677.0 19 710.5 20 744.0 21 910.0 22 1327.0 23 1578.0 24 1796.5 25 2015.0 26 2177.0 27 2339.0 28 2516.5 29 2656.0 30 2618.0 31 2167.5 32 1717.0 33 2036.5 34 2356.0 35 2039.0 36 1722.0 37 1606.5 38 1579.5 39 1668.0 40 2167.5 41 2667.0 42 3808.5 43 4950.0 44 25533.5 45 46117.0 46 26521.5 47 5822.0 48 4718.0 49 6420.0 50 8122.0 51 7852.5 52 7583.0 53 7693.0 54 8311.0 55 8819.0 56 10413.5 57 12008.0 58 13302.5 59 14597.0 60 14240.0 61 13883.0 62 12398.0 63 10113.0 64 9313.0 65 8358.5 66 7404.0 67 6649.5 68 5895.0 69 5205.5 70 4516.0 71 4060.5 72 2963.0 73 2321.0 74 1836.5 75 1352.0 76 1107.0 77 862.0 78 726.5 79 426.5 80 262.0 81 195.5 82 129.0 83 87.0 84 45.0 85 39.0 86 33.0 87 23.5 88 8.0 89 2.0 90 1.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03243485602262808 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00906268035926373 25 0.023372175663364355 26 0.068685577459683 27 0.07011652699009306 28 0.10779819795755803 29 0.17886869130125782 30 0.2637716967722548 31 0.3062231995077534 32 0.30956208174537686 33 0.3329342574087412 34 0.35153660130407205 35 0.3982809526308007 36 0.4273769264158053 37 0.436439606775069 38 0.47793714315696084 39 0.5242045113068863 40 0.5909821560593558 41 0.6143543317227201 42 0.6367725410324778 43 0.6754081783535495 44 0.6725462792927293 45 0.7087970007297842 46 0.7364620249843787 47 0.7817754267806974 48 0.8313816771682463 49 0.7989468211456182 50 0.8681093817821046 51 0.8337665930522631 52 0.8576157518924308 53 0.8642935163676778 54 1.6436840272643582 55 0.9086529518103896 56 0.8910045742686655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 209651.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.754444290749866 #Duplication Level Percentage of deduplicated Percentage of total 1 69.16109620484823 21.270110803191972 2 12.283449912371854 7.555413520565129 3 5.878065046450672 5.423298720254137 4 3.5051258588333827 4.311927918302322 5 2.0069171952789366 3.0860811539177013 6 1.5540425267924995 2.867622858941765 7 1.0003567163484655 2.1535790432671438 8 0.7165345782216914 1.7629298214651967 9 0.5660933356080463 1.5668897357990184 >10 2.957643810971354 16.878049711186684 >50 0.22643733424321852 4.822299917481911 >100 0.1364827768041317 7.789612260375576 >500 0.0031018812910029933 0.5828734420536987 >1k 0.0031018812910029933 1.158592136455347 >5k 0.0 0.0 >10k+ 0.0015509406455014967 18.770718956742396 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 39353 18.770718956742396 No Hit TAGCATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 1239 0.5909821560593558 No Hit TAGCATGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 1190 0.5676099803959914 No Hit TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 645 0.3076541490381634 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 577 0.2752192930155353 Illumina Single End Adapter 1 (95% over 21bp) TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 474 0.22609002580478987 No Hit TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 469 0.22370510992077308 No Hit TAGCATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 446 0.21273449685429596 No Hit TAGCATGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 414 0.19747103519658862 No Hit TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 403 0.19222422025175173 No Hit TAGCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 385 0.18363852306929135 No Hit TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 356 0.16980601094199407 No Hit TAGCATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 342 0.16312824646674712 No Hit TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 329 0.1569274651683035 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG 325 0.1550195324610901 No Hit TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 325 0.1550195324610901 No Hit TAGCATGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC 318 0.15168065022346663 No Hit TAGCATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 301 0.1435719362178096 No Hit TAGCATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA 298 0.14214098668739952 No Hit TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 277 0.1321243399745291 No Hit TAGCATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 272 0.1297394240905123 No Hit TAGCATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 266 0.1268775250296922 No Hit TAGCATGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 250 0.11924579420083854 No Hit TAGCATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 244 0.1163838951400184 No Hit TAGCATGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC 229 0.1092291474879681 No Hit TAGCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 222 0.10589026525034463 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGC 217 0.10350534936632784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 9.539663536067083E-4 0.0 0.0 0.0 0.0 9 9.539663536067083E-4 0.0 0.0 0.0 0.0 10 0.0057237981216402495 0.0 0.0 0.0 0.0 11 0.008108714005657021 0.0 0.0 0.0 0.0 12 0.008585697182460375 0.0 0.0 0.0 0.0 13 0.010970613066477146 0.0 0.0 0.0 0.0 14 0.015740444834510686 0.0 0.0 0.0 0.0 15 0.016694411188117396 0.0 0.0 0.0 0.0 16 0.01717139436492075 0.0 0.0 0.0 0.0 17 0.020033293425740874 0.0 0.0 0.0 0.0 18 0.02337217566336435 0.0 0.0 0.0 0.0 19 0.024326142016971062 0.0 0.0 0.0 0.0 20 0.026711057900987833 0.0 0.0 0.0 0.0 21 0.030526923315414664 0.0 0.0 0.0 0.0 22 0.03577373826025156 0.0 0.0 0.0 0.0 23 0.038158654144268334 0.0 0.0 0.0 0.0 24 0.040543570028285104 0.0 0.0 0.0 0.0 25 0.043405469089105225 0.0 0.0 0.0 0.0 26 0.045790384973121996 0.0 0.0 0.0 0.0 27 0.048652284033942124 0.0 0.0 0.0 0.0 28 0.0572379812164025 0.0 0.0 0.0 0.0 29 0.06009988027722262 0.0 0.0 0.0 0.0 30 0.061530829807632685 0.0 0.0 0.0 0.0 31 0.06630066157566622 0.0 0.0 0.0 0.0 32 0.06916256063648635 0.0 0.0 0.0 0.0 33 0.07107049334369976 0.0 0.0 0.0 0.0 34 0.07440937558132325 0.0 0.0 0.0 0.0 35 0.08299507276378362 0.0 0.0 0.0 0.0 36 0.08728792135501381 0.0 0.0 0.0 0.0 37 0.08967283723903058 0.0 0.0 0.0 0.0 38 0.09301171947665406 0.0 0.0 0.0 0.0 39 0.09921250077509766 0.0 0.0 0.0 0.0 40 0.10112043348231108 0.0 0.0 0.0 0.0 41 0.10350534936632785 0.0 0.0 0.0 0.0 42 0.10684423160395133 0.0 0.0 0.0 0.0 43 0.10970613066477146 0.0 0.0 0.0 0.0 44 0.11304501290239494 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGATCG 20 3.8729643E-4 50.768803 50 GTACGCT 20 3.8729643E-4 50.768803 49 CTATCTC 15 0.007928325 50.7688 49 ATGAACC 15 0.007928325 50.7688 49 CTTAGAT 15 0.007928325 50.7688 49 CTAACTT 15 0.0081830975 50.364315 47 TCCTTAT 15 0.0081830975 50.364315 47 ATCCTTA 15 0.008190912 50.35216 46 ACCCTAT 15 0.008190912 50.35216 46 ACATTTG 25 1.9968564E-5 50.352158 46 TTAAGCA 50 7.2759576E-12 50.34001 45 ATGCCCT 15 0.0082065575 50.327866 44 GACCACT 20 4.0530268E-4 50.303596 42 TCTCAAC 15 0.008222224 50.303593 43 TATCCCC 15 0.008293003 50.194656 39 GAGATCA 15 0.008308793 50.170517 38 GTACATC 15 0.00833252 50.134342 35 ACAGAAC 15 0.00833252 50.134342 36 ACGCACA 25 2.0575124E-5 50.098225 34 ATATGCC 75 0.0 50.098225 34 >>END_MODULE