FastQCFastQC Report
Fri 17 Jun 2016
SRR1526870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70513
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8491.2040332988243303No Hit
CCGCTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3250.4609079176889368No Hit
CCGCTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG1430.2027994837831322No Hit
CCGCTAGGGGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTCTTTTTTTCTTTCTT1000.1418178208273652No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.12763603874462864No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC890.126217860536355No Hit
CCGCTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG870.1233815041198077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCT150.00447741858.61627258
CGAGAGA150.00447741858.61627258
TTGGAAA150.00461960758.15571648
CAACGAT150.00461960758.15571646
ATTACGC150.00461960758.15571651
ACGATTA150.00461960758.15571648
AACGATT150.00461960758.15571647
ACTGTTC150.00461960758.15571646
TACTATT150.00461960758.15571646
GTTGGGT150.00461960758.15571648
GATTACG150.00461960758.15571650
AGATTAA1600.058.15571249
AAGATTA1700.058.15571248
ACAAATA150.00464581658.07275433
AAATATA150.00464581658.07275435
TGAACTG150.00464581658.07275436
ACACAAA150.00464581658.07275431
ATGTCCC150.00464581658.07275443
GTAATGA150.00464581658.07275432
TTATACT150.00464581658.07275443
Produced by FastQC (version 0.11.5)