FastQCFastQC Report
Fri 17 Jun 2016
SRR1526868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104760
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT19091.8222604047346314No Hit
CCGCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG11841.1302023673157695No Hit
CCGCTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG7500.715922107674685No Hit
CCGCTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT7130.6806032836960672No Hit
CCGCTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4000.3818251240931653No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3480.3321878579610538No Hit
CCGCTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT3390.32359679266895763No Hit
CCGCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC3170.3025964108438335No Hit
CCGCTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2950.2815960290187094No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2810.2682321496754487No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2790.2663230240549828No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2590.24723176785032458No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2590.24723176785032458No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2360.22527682321496753No Hit
CCGCTAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT2220.21191294387170675No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA2200.21000381825124095No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2150.20523100420007634No Hit
CCGCTAGGGACCACACCCAGCCTATGTGGGTTTCTGTAGGATACAAACCTGATGTCTCCAGGTG2070.19759450171821305No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1990.18995799923634976No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1880.17945780832378772No Hit
CCGCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTCCA1840.17563955708285603No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1730.165139366170294No Hit
CCGCTAGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG1710.16323024054982818No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1670.15941198930889652No Hit
CCGCTAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA1670.15941198930889652No Hit
CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCA1620.15463917525773196No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG1600.15273004963726614No Hit
CCGCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1600.15273004963726614No Hit
CCGCTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1560.14891179839633448No Hit
CCGCTAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTC1550.14795723558610158No Hit
CCGCTAGGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGT1540.14700267277586865No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1360.1298205421916762No Hit
CCGCTAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGC1320.12600229095074456No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1300.12409316533027873No Hit
CCGCTAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGT1260.12027491408934708No Hit
CCGCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1230.11741122565864834No Hit
CCGCTAGGGGGTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG1210.11550210003818252No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1210.11550210003818252No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG1210.11550210003818252No Hit
CCGCTAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC1150.10977472317678504No Hit
CCGCTAGGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGTGTTGG1150.10977472317678504No Hit
CCGCTAGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT1120.10691103474608629No Hit
CCGCTAGGGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTG1100.10500190912562048No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.10404734631538756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTC201.9240896E-458.4664145
CGAGTTT150.004529288558.46640843
TAGGTGA150.004529288558.46640839
CATTAAG150.004529288558.46640845
ACAATTA150.004529288558.46640843
CCAGTTA150.004529288558.46640844
GCTAACC150.004529288558.46640839
GTGAACT258.214836E-658.46640844
TACAAAC303.5273843E-758.46640842
AACACTC150.004529288558.46640839
CGATACT201.9379331E-458.38175626
TTATGAA150.0045640858.35359223
TAAGTGA150.0045640858.35359223
ACTAAGG150.0045640858.35359222
ATGAAGC150.0045640858.35359225
ATTATGA150.0045640858.35359222
ACATCAA150.0045640858.35359225
GCGCTAA150.0045640858.35359225
ATACATC150.0045640858.35359223
AGGCTAA406.712071E-1058.35359224
Produced by FastQC (version 0.11.5)