FastQCFastQC Report
Fri 17 Jun 2016
SRR1526867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96415
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG21352.2143857283617696No Hit
CCGCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA9090.9427993569465332No Hit
CCGCTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC7670.7955193693927294No Hit
CCGCTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC6140.6368303687185604No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4000.41487320437691233No Hit
CCGCTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC3950.4096872893222009No Hit
CCGCTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3490.361976870818856No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3260.33812166156718354No Hit
CCGCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG3210.3329357465124721No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2950.30596898822797286No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2870.2976715241404346No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2720.28211377897630036No Hit
CCGCTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT2650.2748534978997044No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2490.2582585697246279No Hit
CCGCTAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA2140.22195716434164808No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG2090.2167712492869367No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2080.2157340662759944No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1870.19395322304620652No Hit
CCGCTAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT1840.19084167401337965No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT1780.18461857594772596No Hit
CCGCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCC1730.17943266089301457No Hit
CCGCTAGGGACCACACCCAGCCTATGTGGGTTTCTGTAGGATACAAACCTGATGTC1720.1783954778820723No Hit
CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA1640.17009801379453404No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1630.16906083078359177No Hit
CCGCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC1580.16387491572888035No Hit
CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG1550.1607633666960535No Hit
CCGCTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC1450.1503915365866307No Hit
CCGCTAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1390.14416843852097702No Hit
CCGCTAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC1380.14313125551003475No Hit
CCGCTAGGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTC1340.1389825234662656No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1300.13483379142249652No Hit
CCGCTAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT1270.13172224238966967No Hit
CCGCTAGGGATGTGTGTGTGTATATGTATGTGAGTGTGTAGGTGAGTGTCTGTGTG1220.12653632733495826No Hit
CCGCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1200.12446196131307369No Hit
CCGCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1190.12342477830213142No Hit
CCGCTAGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG1180.12238759529118913No Hit
CCGCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.11823886324742001No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1100.11409013120365087No Hit
CCGCTAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG1100.11409013120365087No Hit
CCGCTAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGC1040.1078670331379972No Hit
CCGCTAGGGGGTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1040.1078670331379972No Hit
CCGCTAGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT1030.10682985012705493No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1020.10579266711611263No Hit
CCGCTAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT1010.10475548410517035No Hit
CCGCTAGGGGACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCTCTACCGGAC1000.10371830109422808No Hit
CCGCTAGGGGGGGAACCTCACGACCTTTCCGTCCCCACGCTAACCCCTTCTCCCGA990.10268111808328578No Hit
CCGCTAGGGGATAAATGGGATGTGTGCCCACCCCTGACATCTGTCCATGTTGTCTA990.10268111808328578No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT990.10268111808328578No Hit
CCGCTAGGGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT990.10268111808328578No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG980.10164393507234351No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC970.10060675206140124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATGT354.6751666E-850.7052349
TCAACAG354.6751666E-850.7052350
TCTTGGT150.00795449450.70522749
CATATTA203.8862458E-450.70522750
GTAGCTA150.00795449450.70522750
CGGTATT150.00795449450.70522749
GCATATT203.8862458E-450.70522749
TTAAGCA204.0617067E-450.2539345
TAAGCAT204.0617067E-450.2539346
AAAGCTA204.0617067E-450.2539345
AAGCTAA204.0617067E-450.2539346
CAAACCT355.0169547E-850.25392544
TAGAAAT150.00824084250.25392545
ACCTGAT355.0169547E-850.25392547
TTCGGTA150.00824084250.25392547
GGTCTTG150.00824084250.25392547
GAATAGA150.00825792250.2276342
TAAGGTT150.00827502750.2013641
GGATACA355.1004463E-850.14890739
CTAAGGT150.00830931850.14890340
Produced by FastQC (version 0.11.5)