##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526867_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96415 Sequences flagged as poor quality 0 Sequence length 56 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.65381942643779 39.0 39.0 40.0 36.0 40.0 2 38.778177669449775 39.0 39.0 40.0 38.0 40.0 3 38.309049421770474 39.0 38.0 40.0 35.0 40.0 4 38.25820671057408 39.0 38.0 40.0 35.0 40.0 5 38.51961831665197 39.0 39.0 40.0 36.0 40.0 6 38.566447129596014 39.0 39.0 40.0 36.0 40.0 7 38.81686459575792 39.0 39.0 40.0 38.0 40.0 8 38.830161281958205 39.0 39.0 40.0 38.0 40.0 9 38.857584400767514 39.0 39.0 40.0 38.0 40.0 10 38.291220245812376 39.0 38.0 40.0 35.0 40.0 11 38.108022610589636 39.0 38.0 40.0 35.0 40.0 12 38.0254213555982 39.0 38.0 40.0 35.0 40.0 13 37.8319141212467 39.0 38.0 40.0 35.0 40.0 14 38.013607841103564 39.0 38.0 40.0 35.0 40.0 15 38.00156614634652 39.0 38.0 40.0 35.0 40.0 16 37.89148991339522 39.0 38.0 40.0 35.0 40.0 17 37.744728517346886 39.0 38.0 40.0 34.0 40.0 18 37.67689674843126 39.0 38.0 40.0 33.0 40.0 19 37.63297204791785 39.0 38.0 40.0 33.0 40.0 20 37.522989161437536 39.0 37.0 40.0 33.0 40.0 21 37.3910802261059 39.0 37.0 40.0 33.0 40.0 22 37.28587875330602 39.0 37.0 40.0 33.0 40.0 23 37.178239900430434 39.0 36.0 40.0 33.0 40.0 24 37.02299434735259 39.0 36.0 40.0 33.0 40.0 25 36.86294663693409 39.0 36.0 40.0 32.0 40.0 26 36.46851630970285 38.0 36.0 40.0 31.0 40.0 27 36.25592490795001 38.0 35.0 40.0 31.0 40.0 28 35.943722449826275 38.0 35.0 40.0 30.0 40.0 29 35.87580770626977 38.0 35.0 40.0 30.0 40.0 30 35.5295545298968 38.0 35.0 40.0 29.0 40.0 31 34.811595706062334 38.0 34.0 39.0 27.0 40.0 32 34.607270652906706 38.0 33.0 39.0 27.0 40.0 33 34.318819685733544 38.0 33.0 39.0 26.0 40.0 34 34.5724835347197 38.0 33.0 39.0 27.0 40.0 35 34.268184411139345 38.0 33.0 39.0 26.0 40.0 36 33.897671524140435 38.0 33.0 39.0 23.0 40.0 37 33.57310584452627 38.0 33.0 39.0 22.0 40.0 38 33.29507856661308 37.0 33.0 39.0 20.0 40.0 39 33.16171757506612 37.0 33.0 39.0 19.0 40.0 40 32.81778768863766 37.0 32.0 39.0 16.0 40.0 41 32.20707358813463 36.0 31.0 39.0 4.0 40.0 42 31.97195457138412 36.0 31.0 39.0 4.0 40.0 43 31.803837577140488 36.0 31.0 39.0 4.0 40.0 44 31.580034227039363 36.0 31.0 39.0 4.0 40.0 45 31.173313281128454 36.0 30.0 39.0 4.0 40.0 46 30.46092413006275 36.0 29.0 39.0 4.0 40.0 47 30.140994658507495 35.0 29.0 39.0 4.0 40.0 48 29.895337862365814 35.0 29.0 38.0 4.0 40.0 49 29.6005497069958 35.0 28.0 38.0 4.0 40.0 50 29.132583104288752 35.0 27.0 38.0 4.0 40.0 51 28.040024892392264 34.0 23.0 38.0 4.0 40.0 52 27.809096095005962 34.0 23.0 38.0 4.0 40.0 53 27.406918010682986 33.0 20.0 38.0 4.0 40.0 54 26.805631903749415 33.0 11.0 38.0 4.0 39.0 55 26.454732147487423 33.0 4.0 38.0 4.0 39.0 56 15.833864025307266 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.3749055133613695 1 2 -0.7896334125447311 1 3 -1.094863964522034 1 4 -0.7382657600028821 1 5 -1.1716483024954911 1 6 -0.7156422659253749 1 7 -0.728689086583536 1 8 -1.16657099553656 1 9 -1.0971528828705317 1 10 -0.5725392663189623 1 11 -0.5749315610535035 1 12 -1.3984592587038023 1 13 -0.8851882746627382 1 14 -0.8725523407413434 1 15 -1.0350666590572075 1 16 -0.5748580218399155 1 17 -1.647101637710776 1 18 -1.4067460135934624 1 19 -0.8771406756076061 1 20 -1.6958573000159873 1 21 -1.581374229964176 1 22 -1.4640761016298498 1 23 -1.1044650319212934 1 24 -1.6533693641743739 1 25 -1.6736799592069858 1 26 -1.4787613093222731 1 27 -1.331142765418278 1 28 -0.9751424641052608 1 29 -0.5986157907203022 1 30 -1.0635122845645455 1 31 -0.2287600757166004 1 32 -0.6168778577835496 1 33 -1.7004257605063273 1 34 -2.042399701069428 1 35 -1.8424118485942387 1 36 -0.20455327183016436 1 37 -0.26721465780381237 1 38 -0.49226393078332364 1 39 -0.8206332302701043 1 40 -1.070232354275344 1 41 0.13019495558145167 1 42 -0.17573589758654862 1 43 -0.10628884089868151 1 44 0.5774754484967701 1 45 0.38388848887024807 1 46 -0.3456255067779104 1 47 0.28795712343221425 1 48 -0.5240685202551312 1 49 0.17765725399640786 1 50 0.14131394738637582 1 51 0.41223320759992177 1 52 0.9443499049716415 1 53 0.7556781513462454 1 54 0.427468001112036 1 55 0.007856598850519703 1 56 1.0531067560740013 2 1 -0.3711150706918147 2 2 -0.14837173662758119 2 3 -0.647997848372043 2 4 -0.26838624193059246 2 5 -0.623793963792366 2 6 0.09266963335714706 2 7 -0.05940520950217376 2 8 -0.0314687889574401 2 9 -0.10338003868479717 2 10 0.16605556243425212 2 11 -0.6347664060514759 2 12 -0.28420448680722643 2 13 -0.30809337822304883 2 14 -0.13910168418252056 2 15 0.0027023946858548697 2 16 -0.7741270078964959 2 17 -0.5957952887193017 2 18 -0.705514119725855 2 19 -0.5189438433346965 2 20 -0.41319857522919534 2 21 -0.7111968913964191 2 22 -0.8772343526113033 2 23 -0.6012837675379217 2 24 -0.874298022628416 2 25 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10 -0.10481121124151116 3 11 -0.4029579523329119 3 12 -0.3346325231903222 3 13 0.40124316538315696 3 14 -0.14779617321408978 3 15 -0.5581590285351226 3 16 0.01693773260644349 3 17 -0.02891460386108946 3 18 -0.005139587890653274 3 19 0.0432551935834411 3 20 -0.03880050139292024 3 21 -0.05469608882820154 3 22 0.026457461192869403 3 23 0.014152294527356446 3 24 0.36714124970973927 3 25 -0.17511426787020667 3 26 0.11805452544537332 3 27 0.08753193337690135 3 28 -0.330851012584894 3 29 0.1850904628654675 3 30 -0.5683889340195094 3 31 -0.35340149855079517 3 32 -0.06782450150127772 3 33 0.07869070535712552 3 34 -0.38103997645669097 3 35 -0.2392850557084074 3 36 0.20408126632244716 3 37 -0.1745583181595194 3 38 0.33275041230331226 3 39 -0.13388624231829738 3 40 -0.34045610642680657 3 41 -0.6715835871609492 3 42 -0.45858156597438793 3 43 -0.7448430577661505 3 44 -0.7095297150144155 3 45 -1.1572761697642875 3 46 -0.21452969495920726 3 47 -0.6923928478816102 3 48 -0.3836497021254708 3 49 0.4557697037956068 3 50 1.0440678200197695 3 51 0.8836904652017594 3 52 2.019794540657241 3 53 0.9805777497398189 3 54 1.3907496993334938 3 55 1.0031233802618793 3 56 0.24559097867870605 4 1 -0.08383212891448721 4 2 0.18832387113544513 4 3 0.34804403109680493 4 4 0.10661375806940754 4 5 0.43213044887362173 4 6 -0.08174303262855176 4 7 0.3581126658808671 4 8 0.3412329475083453 4 9 0.3824473361875249 4 10 0.262071905641605 4 11 -0.25414842852338637 4 12 0.05389561533782228 4 13 0.28868905282904933 4 14 0.2456020951841822 4 15 0.36608339570730664 4 16 0.5678035334722438 4 17 0.7806092056627207 4 18 0.18046647271540905 4 19 0.7542941546224071 4 20 0.3605501479577313 4 21 0.6709532618211469 4 22 0.46801590275131133 4 23 0.6662085715836312 4 24 0.5467949293634149 4 25 0.6717472039912664 4 26 0.4605869929778379 4 27 -0.6397407938958253 4 28 0.4635212817874006 4 29 0.5281640559390652 4 30 -0.0981508387814145 4 31 0.3581785880292898 4 32 0.08314952447274493 4 33 0.07490282656924308 4 34 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8 15 -0.13953157755472745 8 16 0.21833829283053774 8 17 -0.24880255904316328 8 18 -0.683570960439674 8 19 0.3419946893817638 8 20 -1.1266156274433428 8 21 -0.0933515510130718 8 22 -0.1886685892272979 8 23 -0.1523743161169051 8 24 0.4106986726789259 8 25 0.13524987778805553 8 26 0.653768811159658 8 27 0.11988487455337093 8 28 0.31004534595852107 8 29 0.9738859810727547 8 30 1.0915550435715247 8 31 1.4307721709169954 8 32 2.280074658162583 8 33 2.1218279602590826 8 34 1.9000524605181042 8 35 1.4908269891095216 8 36 2.3207479329891143 8 37 2.3041531664343182 8 38 2.0442350061408447 8 39 2.195525522387584 8 40 2.772148935589996 8 41 3.0160914828670613 8 42 3.0416985460704318 8 43 2.9022157657632626 8 44 3.104335831204068 8 45 2.9260571635690447 8 46 4.654657980110816 8 47 4.515030121306065 8 48 4.824053379107024 8 49 5.2920442135995245 8 50 5.607933366238253 8 51 4.838172257918842 8 52 5.18758165550318 8 53 6.070773828171191 8 54 4.531365945832093 8 55 4.492619178581208 8 56 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1.3817533661006927 120 46 0.7423720530817803 120 47 1.5334962419315303 120 48 0.448327392509853 120 49 -0.23304886160523708 120 50 1.061246843527904 120 51 0.9133770233693284 120 52 2.1677752519290934 120 53 1.4067380872925632 120 54 2.2671812846258383 120 55 2.306468769050305 120 56 2.0825409485400677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 11.0 5 0.0 6 9.0 7 24.0 8 19.0 9 21.0 10 40.0 11 59.0 12 88.0 13 114.0 14 177.0 15 251.0 16 322.0 17 408.0 18 530.0 19 644.0 20 694.0 21 911.0 22 965.0 23 970.0 24 1387.0 25 1655.0 26 1597.0 27 1742.0 28 1923.0 29 2088.0 30 2470.0 31 2855.0 32 3469.0 33 4330.0 34 5588.0 35 7671.0 36 10926.0 37 14961.0 38 16818.0 39 10678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.68054763262978 0.2841881449981849 0.017632111186018772 0.017632111186018772 8 99.85578818500612 0.12553690369970744 0.009337455647085676 0.009337455647085676 9 99.73136960016595 0.12757351034590053 0.035264222372037544 0.10579266711611263 10 73.32054140953171 12.336254732147488 6.2282839807083965 8.114919877612405 11 41.651195353420114 30.185137167453195 11.82803505678577 16.33563242234092 12 34.38054244671472 12.330031634081834 23.448633511383083 29.84079240782036 13 25.869418658922367 16.540994658507493 25.932686822589844 31.656899859980292 14 24.28875175024633 17.0440284188145 32.36944458849764 26.297775242441528 15 27.76227765389203 18.875693616138566 27.914743556500543 25.44728517346886 16 32.158896437276354 18.552092516724574 22.038064616501583 27.250946429497485 17 30.751439091427685 17.075143909142767 25.54270601047555 26.630710988954 18 28.693667997718197 19.91806254213556 25.84348908364881 25.544780376497435 19 33.16911268993414 19.1806254213556 20.24062645853861 27.409635430171654 20 29.669657211014883 22.960120313229268 21.91360265518851 25.45661982056734 21 28.528755898978375 18.634029974589016 26.186796660270705 26.6504174661619 22 29.884354094279935 17.28984079240782 24.935953949074314 27.88985116423793 23 29.217445418244047 18.74812010579267 22.920707358813463 29.113727117149825 24 27.86859739020393 21.335808974545152 24.80862187000809 25.986971765242828 25 31.787200049797182 19.47484723677522 20.7145895363675 28.0233631770601 26 25.994083761482173 19.313923919248534 23.405469925787532 31.286522393481757 27 25.988624333132666 18.394121188217465 23.55157453345235 32.065679945197516 28 29.242461998504858 20.263310906221445 23.570271617243957 26.923955478029736 29 30.911470777669276 19.3066838420145 21.844549251002473 27.93729612931375 30 27.282369088716052 17.825354573056607 28.488541363390592 26.403734974836752 31 29.53427165579586 18.653059950691258 24.787525096484934 27.025143297027952 32 33.37321196358908 18.289726918075424 22.87230169050715 25.464759427828348 33 31.4215553659501 18.57428256300335 23.691548914739975 26.31261315630658 34 27.157487198701137 20.959785187960534 26.876483077307356 25.006244536030977 35 27.102054416526965 21.755156866624322 30.90683798952487 20.235950727323843 36 30.204068877152412 22.804879319145392 24.338260570642834 22.652791233059368 37 28.254914011312383 20.573744023499753 27.338256893157364 23.8330850720305 38 31.23430155603498 21.904345016623413 23.582319774046628 23.27903365329498 39 30.593007372185898 19.971637417753726 22.110301248162166 27.32505396189821 40 29.340192630777096 19.379950120524676 25.667059719714914 25.612797528983318 41 28.74157115717835 19.616500699359094 25.56835974196781 26.07356840149475 42 26.94853324981731 19.137697045620627 24.943104708215888 28.970664996346173 43 23.82752535009764 16.811996783592143 30.654037740577913 28.706440125732307 44 26.045365303479677 16.886670286979406 26.350307030368853 30.717657379172064 45 26.021101013266478 17.71858351613914 28.627389533061738 27.632925937532644 46 25.606837964075613 18.4459932498093 26.483526817901588 29.4636419682135 47 25.2362533974493 21.57118962993937 27.942713777963625 25.24984319464771 48 26.216272746287387 20.19661158753399 27.623928048525414 25.96318761765321 49 26.822867657821554 20.91357714907053 24.981703364489892 27.28185182861803 50 27.000564865373754 19.85397184041507 24.885457854766834 28.260005439444342 51 26.227279382543035 23.560417494613986 26.42389508251585 23.788408040327134 52 27.64486196399243 20.032220606542456 25.997217311253152 26.325700118211966 53 27.71855679809191 19.181320808008955 27.28128628665279 25.818836107246344 54 27.47135652944528 21.25419365729116 24.978372333466968 26.296077479796594 55 27.562821170289592 20.46384577546599 24.777862667322527 27.195470386921894 56 28.08337431593927 18.9287321202482 23.07233517144681 29.915558392365725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 9.0 14 17.0 15 39.0 16 61.0 17 87.0 18 113.0 19 139.0 20 165.0 21 226.0 22 264.0 23 241.0 24 285.0 25 329.0 26 450.0 27 571.0 28 625.0 29 849.0 30 1019.0 31 1017.5 32 1016.0 33 1135.0 34 1254.0 35 1187.0 36 1120.0 37 938.0 38 772.5 39 789.0 40 870.5 41 952.0 42 887.0 43 822.0 44 947.0 45 1072.0 46 1460.5 47 3524.0 48 5199.0 49 3748.5 50 2298.0 51 2265.5 52 2233.0 53 3133.0 54 3829.0 55 3625.0 56 3696.0 57 3767.0 58 4137.0 59 4507.0 60 5740.0 61 6973.0 62 7959.0 63 9108.0 64 9271.0 65 8643.5 66 8016.0 67 7060.5 68 6105.0 69 5515.5 70 4926.0 71 4765.5 72 3809.5 73 3014.0 74 2702.0 75 2390.0 76 1918.0 77 1446.0 78 1130.5 79 645.5 80 476.0 81 393.0 82 310.0 83 243.0 84 176.0 85 144.5 86 113.0 87 77.0 88 26.5 89 12.0 90 7.5 91 3.0 92 2.5 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.030078307317326143 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.009334647098480527 25 0.02489239226261474 26 0.07260281076595965 27 0.07156562775501737 28 0.10682985012705493 29 0.15868900067416897 30 0.2520354716589742 31 0.2966343411294923 32 0.3007830731732614 33 0.3204895503811647 34 0.3433075766218949 35 0.3920551781361821 36 0.4335424985738734 37 0.4293937665301042 38 0.48332728309910283 39 0.5331120676243324 40 0.605714878390292 41 0.6357931857076181 42 0.6482393818389255 43 0.6803920551781362 44 0.683503604210963 45 0.7104703624954624 46 0.7405486698127884 47 0.783073173261422 48 0.8245604936991132 49 0.7975937354146139 50 0.8473785199398435 51 0.8266348597209977 52 0.8546388010164394 53 0.8525644349945547 54 1.6895711248249754 55 0.8982004874760151 56 0.8774568272571696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 96415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.2991754395063 #Duplication Level Percentage of deduplicated Percentage of total 1 68.04126578054614 25.37883109474667 2 12.482620543907458 9.311829072239796 3 5.961848617985652 6.671161126380749 4 3.740058951115066 5.5800445988694705 5 2.257938935543073 4.210963024425659 6 1.6767699238084646 3.7525281335891716 7 1.1261887547967298 2.9404138360213663 8 0.8342138924420222 2.489239226261474 9 0.5867304376842223 1.9696105377793913 >10 2.967020744118792 20.062230980656537 >50 0.20299204716089206 5.302079551936939 >100 0.11122851899226963 7.741533993673183 >500 0.008342138924420222 2.375149095057823 >1k 0.002780712974806741 2.2143857283617696 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2135 2.2143857283617696 No Hit CCGCTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 909 0.9427993569465332 No Hit CCGCTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 767 0.7955193693927294 No Hit CCGCTAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 614 0.6368303687185604 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 400 0.41487320437691233 No Hit CCGCTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 395 0.4096872893222009 No Hit CCGCTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 349 0.361976870818856 No Hit CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 326 0.33812166156718354 No Hit CCGCTAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG 321 0.3329357465124721 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 295 0.30596898822797286 No Hit CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 287 0.2976715241404346 No Hit CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 272 0.28211377897630036 No Hit CCGCTAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 265 0.2748534978997044 No Hit CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 249 0.2582585697246279 No Hit CCGCTAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA 214 0.22195716434164808 No Hit CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 209 0.2167712492869367 No Hit CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 208 0.2157340662759944 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 187 0.19395322304620652 No Hit CCGCTAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 184 0.19084167401337965 No Hit CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT 178 0.18461857594772596 No Hit CCGCTAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCC 173 0.17943266089301457 No Hit CCGCTAGGGACCACACCCAGCCTATGTGGGTTTCTGTAGGATACAAACCTGATGTC 172 0.1783954778820723 No Hit CCGCTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA 164 0.17009801379453404 No Hit CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 163 0.16906083078359177 No Hit CCGCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 158 0.16387491572888035 No Hit CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG 155 0.1607633666960535 No Hit CCGCTAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC 145 0.1503915365866307 No Hit CCGCTAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 139 0.14416843852097702 No Hit CCGCTAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC 138 0.14313125551003475 No Hit CCGCTAGGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTC 134 0.1389825234662656 No Hit CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 130 0.13483379142249652 No Hit CCGCTAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT 127 0.13172224238966967 No Hit CCGCTAGGGATGTGTGTGTGTATATGTATGTGAGTGTGTAGGTGAGTGTCTGTGTG 122 0.12653632733495826 No Hit CCGCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 120 0.12446196131307369 No Hit CCGCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 119 0.12342477830213142 No Hit CCGCTAGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG 118 0.12238759529118913 No Hit CCGCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.11823886324742001 No Hit CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 110 0.11409013120365087 No Hit CCGCTAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG 110 0.11409013120365087 No Hit CCGCTAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGC 104 0.1078670331379972 No Hit CCGCTAGGGGGTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC 104 0.1078670331379972 No Hit CCGCTAGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT 103 0.10682985012705493 No Hit CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 102 0.10579266711611263 No Hit CCGCTAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT 101 0.10475548410517035 No Hit CCGCTAGGGGACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCTCTACCGGAC 100 0.10371830109422808 No Hit CCGCTAGGGGGGGAACCTCACGACCTTTCCGTCCCCACGCTAACCCCTTCTCCCGA 99 0.10268111808328578 No Hit CCGCTAGGGGATAAATGGGATGTGTGCCCACCCCTGACATCTGTCCATGTTGTCTA 99 0.10268111808328578 No Hit CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 99 0.10268111808328578 No Hit CCGCTAGGGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT 99 0.10268111808328578 No Hit CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 98 0.10164393507234351 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 97 0.10060675206140124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0020743660218845617 0.0 0.0 0.0 0.0 7 0.0020743660218845617 0.0 0.0 0.0 0.0 8 0.004148732043769123 0.0 0.0 0.0 0.0 9 0.004148732043769123 0.0 0.0 0.0 0.0 10 0.006223098065653685 0.0 0.0 0.0 0.0 11 0.008297464087538247 0.0 0.0 0.0 0.0 12 0.009334647098480527 0.0 0.0 0.0 0.0 13 0.009334647098480527 0.0 0.0 0.0 0.0 14 0.011409013120365089 0.0 0.0 0.0 0.0 15 0.011409013120365089 0.0 0.0 0.0 0.0 16 0.011409013120365089 0.0 0.0 0.0 0.0 17 0.011409013120365089 0.0 0.0 0.0 0.0 18 0.011409013120365089 0.0 0.0 0.0 0.0 19 0.01244619613130737 0.0 0.0 0.0 0.0 20 0.01348337914224965 0.0 0.0 0.0 0.0 21 0.01348337914224965 0.0 0.0 0.0 0.0 22 0.016594928175076493 0.0 0.0 0.0 0.0 23 0.016594928175076493 0.0 0.0 0.0 0.0 24 0.016594928175076493 0.0 0.0 0.0 0.0 25 0.016594928175076493 0.0 0.0 0.0 0.0 26 0.017632111186018772 0.0 0.0 0.0 0.0 27 0.018669294196961055 0.0 0.0 0.0 0.0 28 0.020743660218845616 0.0 0.0 0.0 0.0 29 0.0269667582844993 0.0 0.0 0.0 0.0 30 0.028003941295441582 0.0 0.0 0.0 0.0 31 0.030078307317326143 0.0 0.0 0.0 0.0 32 0.031115490328268422 0.0 0.0 0.0 0.0 33 0.035264222372037544 0.0 0.0 0.0 0.0 34 0.03837577140486439 0.0 0.0 0.0 0.0 35 0.04252450344863351 0.0 0.0 0.0 0.0 36 0.044598869470518075 0.0 0.0 0.0 0.0 37 0.045636052481460354 0.0 0.0 0.0 0.0 38 0.04667323549240263 0.0 0.0 0.0 0.0 39 0.0487476015142872 0.0 0.0 0.0 0.0 40 0.05289633355805632 0.0 0.0 0.0 0.0 41 0.05704506560182544 0.0 0.0 0.0 0.0 42 0.05911943162371 0.0 0.0 0.0 0.0 43 0.060156614634652286 0.0 0.0 0.0 0.0 44 0.06326816366747913 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGATGT 35 4.6751666E-8 50.70523 49 TCAACAG 35 4.6751666E-8 50.70523 50 TCTTGGT 15 0.007954494 50.705227 49 CATATTA 20 3.8862458E-4 50.705227 50 GTAGCTA 15 0.007954494 50.705227 50 CGGTATT 15 0.007954494 50.705227 49 GCATATT 20 3.8862458E-4 50.705227 49 TTAAGCA 20 4.0617067E-4 50.25393 45 TAAGCAT 20 4.0617067E-4 50.25393 46 AAAGCTA 20 4.0617067E-4 50.25393 45 AAGCTAA 20 4.0617067E-4 50.25393 46 CAAACCT 35 5.0169547E-8 50.253925 44 TAGAAAT 15 0.008240842 50.253925 45 ACCTGAT 35 5.0169547E-8 50.253925 47 TTCGGTA 15 0.008240842 50.253925 47 GGTCTTG 15 0.008240842 50.253925 47 GAATAGA 15 0.008257922 50.22763 42 TAAGGTT 15 0.008275027 50.20136 41 GGATACA 35 5.1004463E-8 50.148907 39 CTAAGGT 15 0.008309318 50.148903 40 >>END_MODULE