FastQCFastQC Report
Fri 17 Jun 2016
SRR1526866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278334
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT77602.7880172742101217No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.26838259070038156No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5930.2130533819080673No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4700.16886187098953057No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.16059841772834077No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT4110.14766431697169588No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.146227194665402No Hit
TACTTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA3070.11029913700805506No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2880.10347280605315916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA150.004514503858.54074558
TCGGCTA150.004645976258.1179165
ACTATTA201.996531E-458.0654947
CGATTAA453.092282E-1158.06548749
AACGATT351.6207196E-858.06548747
ATAGGTC258.603845E-658.0550146
TTAGTCA202.0000956E-458.04454854
AATCGAG150.00467594658.02361742
TAGTGTG202.003665E-458.02361340
TACGTAC407.1304385E-1058.02361340
GATAGTG150.00468596857.99225638
GTACGTG150.00468596857.99225638
CAGCGTA303.7687641E-757.96092635
ATATACT150.0047060657.9296329
GTATATA150.004712771657.90878327
CCAGTTA150.004712771657.90878327
GTACCCT202.023388E-457.9087826
TACGCCT150.00472285257.8775424
CAACTAG407.2941475E-1057.8775416
ACGACCA150.004729581557.85673523
Produced by FastQC (version 0.11.5)