FastQCFastQC Report
Fri 17 Jun 2016
SRR1526863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344727
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG193735.619809298372335No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC22570.6547209821104816No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC17130.49691495009094155No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11150.3234443487165206No Hit
TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG10770.31242113324456744No Hit
TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC8480.24599175579516544No Hit
TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT8420.2442512480890676No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8280.2401900634415059No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT7710.22365524023357614No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.1972575400244251No Hit
TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5470.15867628587258903No Hit
TACTTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA4930.14301171651770822No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4800.13924061648782948No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.13895053187014653No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA4700.13633977031099972No Hit
TACTTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4650.13488934722258483No Hit
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG4530.13140833181038908No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA4340.12589672407441252No Hit
TACTTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC4300.1247363856036806No Hit
TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4250.1232859625152657No Hit
TACTTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC4250.1232859625152657No Hit
TACTTCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT4000.11603384707319125No Hit
TACTTCGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTT3950.11458342398477638No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3940.11429333936709338No Hit
TACTTCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT3900.11313300089636147No Hit
TACTTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT3820.11081232395489765No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3770.10936190086648276No Hit
TACTTCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA3580.10385029313050617No Hit
TACTTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT3560.1032701238951402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA150.00798692750.68143549
ACCGAAC150.00798692750.68143550
TCGTGAT150.00825128350.2657147
TATTCCG150.008275250.22892442
ATGTCGT150.00830876750.17751338
CAGTACG204.116193E-450.1555137
AATGATG150.00834243750.12620534
TATGGAC204.16092E-450.04579532
CAATTAA150.00839555250.0457932
GTACTAG150.00839555250.0457932
TACGAGG150.008424628550.0020429
AGTCCTC252.0892792E-549.98019428
AATGAAC150.00843919549.9801928
CTTATGA150.00847813249.92202425
CAATAAC150.00854168749.8277921
CGGGATA2250.049.798876
AGCGCAT204.2728987E-449.777215
GATATAC150.00857605749.777210
GGACGTA150.00857605749.77729
CTAATCT150.00857605749.777213
Produced by FastQC (version 0.11.5)