##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526863_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344727 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.81583978046396 40.0 39.0 40.0 37.0 40.0 2 38.60178344022952 39.0 39.0 40.0 36.0 40.0 3 38.5300223075071 40.0 39.0 40.0 36.0 40.0 4 38.544062403002954 39.0 39.0 40.0 35.0 40.0 5 38.80832078717362 40.0 39.0 40.0 38.0 40.0 6 38.698422229764425 40.0 39.0 40.0 36.0 40.0 7 38.84336881068207 39.0 39.0 40.0 38.0 40.0 8 38.81751066786182 40.0 39.0 40.0 38.0 40.0 9 38.78424666475211 40.0 39.0 40.0 38.0 40.0 10 38.18923379949931 39.0 38.0 40.0 35.0 40.0 11 38.149674960185884 39.0 38.0 40.0 35.0 40.0 12 38.08169943172423 39.0 38.0 40.0 35.0 40.0 13 37.88330185915231 39.0 38.0 40.0 35.0 40.0 14 38.06828881984875 39.0 38.0 40.0 35.0 40.0 15 38.02544332181697 39.0 38.0 40.0 35.0 40.0 16 37.909571922129686 39.0 38.0 40.0 35.0 40.0 17 37.80666730485282 39.0 38.0 40.0 34.0 40.0 18 37.73093491371433 39.0 38.0 40.0 33.0 40.0 19 37.59693322542186 39.0 38.0 40.0 33.0 40.0 20 37.55303762107407 39.0 38.0 40.0 33.0 40.0 21 37.47331946728861 39.0 38.0 40.0 33.0 40.0 22 37.34779405152483 39.0 37.0 40.0 33.0 40.0 23 37.269752586829576 39.0 37.0 40.0 33.0 40.0 24 37.1328210438985 39.0 37.0 40.0 33.0 40.0 25 36.93142399637974 39.0 36.0 40.0 32.0 40.0 26 36.59909145497742 39.0 36.0 40.0 32.0 40.0 27 36.406588401836814 39.0 36.0 40.0 31.0 40.0 28 36.055586014440415 38.0 35.0 40.0 30.0 40.0 29 35.95821621166894 38.0 35.0 40.0 30.0 40.0 30 35.617613357816474 38.0 35.0 40.0 29.0 40.0 31 35.056209696368434 38.0 35.0 40.0 28.0 40.0 32 34.856425519323984 38.0 34.0 39.0 27.0 40.0 33 34.5842826352447 38.0 33.0 39.0 27.0 40.0 34 34.828179980100195 38.0 34.0 40.0 27.0 40.0 35 34.580653676677485 38.0 34.0 40.0 26.0 40.0 36 34.29374838640432 38.0 34.0 40.0 25.0 40.0 37 34.00543618573538 38.0 33.0 40.0 23.0 40.0 38 33.90784881950065 38.0 33.0 40.0 23.0 40.0 39 33.72776718968923 38.0 33.0 40.0 22.0 40.0 40 33.38326269772893 38.0 33.0 39.0 19.0 40.0 41 33.04876322423251 38.0 33.0 39.0 14.0 40.0 42 32.82986247088276 37.0 32.0 39.0 11.0 40.0 43 32.60482352702283 37.0 32.0 39.0 9.0 40.0 44 32.46773243755204 37.0 32.0 39.0 4.0 40.0 45 32.068761657775575 36.0 31.0 39.0 4.0 40.0 46 31.502687633982834 36.0 31.0 39.0 4.0 40.0 47 31.0933347257395 36.0 30.0 39.0 4.0 40.0 48 30.898676343889512 36.0 30.0 39.0 4.0 40.0 49 30.5828554189257 36.0 30.0 39.0 4.0 40.0 50 30.217084823643056 35.0 29.0 39.0 4.0 40.0 51 29.320703049079416 35.0 28.0 38.0 4.0 40.0 52 29.016630551131765 35.0 27.0 38.0 4.0 40.0 53 28.770389322565393 35.0 26.0 38.0 4.0 40.0 54 28.325054318344662 35.0 24.0 38.0 4.0 40.0 55 27.97892825337151 34.0 23.0 38.0 4.0 40.0 56 16.60813629335677 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.4398758778873386 1 2 -0.5067716154170014 1 3 -0.40248446442195984 1 4 -0.6942223474621372 1 5 -0.8115751951608203 1 6 -0.5856780742447043 1 7 -0.501650845141782 1 8 -0.6310140337383245 1 9 -0.6177884952324249 1 10 -0.4591777141193063 1 11 -0.6815016766493542 1 12 -0.9961786980119882 1 13 -0.6193484567852607 1 14 -0.7419348632810738 1 15 -0.5273888359426522 1 16 -0.481657083671152 1 17 -0.5472486346671914 1 18 -0.7423322785974165 1 19 -0.49195724634458315 1 20 -0.6739127535775324 1 21 -0.4142298350409277 1 22 -0.7045680545663799 1 23 -0.7037011376710396 1 24 -0.7459568164207653 1 25 -0.9690035441974487 1 26 -0.7148919672355802 1 27 -1.346692757915406 1 28 -0.9022038248891207 1 29 -0.5363043536743177 1 30 -0.39174033066781533 1 31 -0.7419185719233639 1 32 -0.21645557563216755 1 33 -0.8758399139879671 1 34 -1.0779180172472067 1 35 -1.0957914230230656 1 36 -0.38003763618192465 1 37 -0.3059202965403358 1 38 -0.4611811611489074 1 39 -0.2593227296480549 1 40 -0.6250173166804274 1 41 -0.14367467682425428 1 42 -0.07324695948697268 1 43 0.32794933244588975 1 44 0.3754844249060554 1 45 0.29442104020937876 1 46 0.03638806117992033 1 47 0.5513124984488087 1 48 0.11248583190976191 1 49 0.3795375742791016 1 50 0.9575647377086973 1 51 1.3954077818483022 1 52 1.5464482588440767 1 53 1.0449635681111786 1 54 1.6953329077023973 1 55 1.380657954977977 1 56 1.935181758078489 2 1 -0.0869358662045201 2 2 -0.12902045951074115 2 3 -0.1626104012753231 2 4 -0.033746916039305574 2 5 -0.00870795359374199 2 6 -0.04857521591376468 2 7 0.05081789341053877 2 8 -0.08654505756929609 2 9 0.013965017039488714 2 10 -0.3103999550428469 2 11 -0.1472997222519581 2 12 -0.36614790684736676 2 13 -0.28266538796552254 2 14 0.1720181876184057 2 15 -0.02576017144753706 2 16 -0.2081899589322873 2 17 -0.2018826554388582 2 18 -0.020225014456748625 2 19 -0.08837517452493415 2 20 -0.1990753725096468 2 21 -0.2255720724002117 2 22 -0.1910288527604962 2 23 0.03803920667516536 2 24 -0.17786477955057478 2 25 -0.49939373062755266 2 26 0.02872950935422125 2 27 -0.11070966830420303 2 28 0.0460848398040099 2 29 -0.20965068992175162 2 30 0.2779922515327087 2 31 0.36613762442723896 2 32 0.5645608298211329 2 33 0.13205094073911994 2 34 0.425175950258712 2 35 0.6665918725226092 2 36 -0.278624806860023 2 37 0.4648884913174527 2 38 0.5752038967206587 2 39 -0.18407743990418624 2 40 0.5806399410247849 2 41 1.0610816175432447 2 42 0.3301965025884144 2 43 1.0183743693259828 2 44 1.056375091519918 2 45 1.0544479701025509 2 46 1.2436096288561238 2 47 1.3837828211124936 2 48 1.1146540843379071 2 49 1.4435950775215787 2 50 1.637950468825597 2 51 1.34424098482247 2 52 1.3483254308092647 2 53 1.3880424865591436 2 54 1.4567360343422031 2 55 1.6402443038983066 2 56 0.8927176728532977 3 1 -0.17318206681242287 3 2 -0.2861670233775939 3 3 -0.21328131831884178 3 4 -0.22731204317993559 3 5 -0.1922556591315896 3 6 -0.14113211412712445 3 7 -0.2025922926670063 3 8 -0.3343488236008838 3 9 -0.2641597512902578 3 10 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50 0.4544861645776912 3 51 0.9924821442857343 3 52 1.1786779402614123 3 53 0.9798765735930246 3 54 1.3004512838047262 3 55 1.2305030332333189 3 56 -0.026346008664976495 4 1 -0.19070543129840445 4 2 -0.07404408439118981 4 3 -0.23725366084600097 4 4 -0.3979677684287779 4 5 -0.20692969605887157 4 6 -0.1167038791905668 4 7 -0.061170668170383635 4 8 0.12455437524419466 4 9 0.027051022967015115 4 10 -0.07355461042259037 4 11 0.017809312015955925 4 12 -0.02762562181484185 4 13 0.2051718228782491 4 14 -0.0897587442718688 4 15 -0.10675705618273668 4 16 0.05184469720497731 4 17 -0.34295017809317585 4 18 -0.2552144566444454 4 19 0.12980698798016022 4 20 0.17784326131891248 4 21 0.01694513730728886 4 22 0.20839733250780768 4 23 -0.00468442483351339 4 24 0.12584039709156514 4 25 0.053797758734148715 4 26 0.2779033487050313 4 27 0.0026329445412898167 4 28 0.7284698638076463 4 29 0.4292247847063564 4 30 0.5834491728178293 4 31 0.9399997005760525 4 32 1.1575690014731705 4 33 0.6065086100665482 4 34 1.0140178103274522 4 35 1.3357488572492855 4 36 0.9299464323336082 4 37 0.9895123396637118 4 38 1.0536290309421759 4 39 0.9466500549115935 4 40 1.0497571564136265 4 41 1.4124153106943922 4 42 1.3524483939991825 4 43 1.3914012893801484 4 44 1.4395147860535218 4 45 1.2463729007429265 4 46 1.3369350083218912 4 47 1.5077495284953848 4 48 1.0651886031762672 4 49 1.1511389421543292 4 50 1.135114536281968 4 51 1.4812652914630036 4 52 1.0425465672654362 4 53 1.5094525936522878 4 54 1.8396295377367977 4 55 1.670895100705394 4 56 1.3456052108472178 5 1 -0.08984873347908717 5 2 -0.12020392143887904 5 3 -0.029729098181256575 5 4 0.05817888865245635 5 5 -0.035829767949600466 5 6 -0.06811773013713207 5 7 0.1469230294092938 5 8 0.22834661104577236 5 9 0.2196582599667778 5 10 -0.19202453613551285 5 11 0.032187457235224315 5 12 -0.026277670700537215 5 13 0.27427678333835104 5 14 -0.03251575361766612 5 15 0.06718854650117834 5 16 -0.0266959210552713 5 17 0.3142209218349379 5 18 -0.2867864671884135 5 19 0.250014272907066 5 20 0.07770547076056289 5 21 0.3190659137271368 5 22 0.34930315565662085 5 23 0.17219671583267626 5 24 0.004554751273211366 5 25 0.23328493822133112 5 26 0.190885615659262 5 27 0.21962311947539348 5 28 0.3256421619145229 5 29 0.6554708906253595 5 30 0.8356358490699236 5 31 0.7309474599817563 5 32 1.0169028140780085 5 33 0.927770292309539 5 34 0.9549475970516355 5 35 1.0410268347235245 5 36 0.9710439640853465 5 37 0.06179247385973241 5 38 0.8239082065951777 5 39 0.307661317792018 5 40 1.074619365855277 5 41 0.78433000036609 5 42 1.257852180244086 5 43 0.6468230483065796 5 44 0.43930510476907614 5 45 1.0682037179008503 5 46 0.367518521382042 5 47 0.2685930463482471 5 48 0.32702061851295383 5 49 0.4187821245171399 5 50 0.2309089288053343 5 51 0.8813970911275106 5 52 0.8003946381976199 5 53 0.6863337343184774 5 54 1.1924931849929692 5 55 0.8398682615009854 5 56 0.6148359800779879 6 1 -0.14642487831402207 6 2 0.03486079552679655 6 3 0.05489447845510398 6 4 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0.20551389597786596 120 45 -0.18027003057289903 120 46 0.029087507951029323 120 47 -0.04593686818166631 120 48 0.19152000801234337 120 49 0.0030006837356992833 120 50 0.06821051610691953 120 51 0.2830454427758653 120 52 0.7323848701878539 120 53 0.5013825743673159 120 54 1.0197215122212953 120 55 0.7750636216963471 120 56 0.9805258457678541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 134.0 5 15.0 6 41.0 7 66.0 8 161.0 9 195.0 10 178.0 11 318.0 12 352.0 13 426.0 14 699.0 15 925.0 16 1167.0 17 1493.0 18 1845.0 19 2231.0 20 2212.0 21 2944.0 22 2996.0 23 3073.0 24 4030.0 25 5011.0 26 5026.0 27 5188.0 28 6210.0 29 6775.0 30 7639.0 31 9184.0 32 10995.0 33 13540.0 34 18034.0 35 24780.0 36 35360.0 37 51242.0 38 65542.0 39 54700.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.45899218802124 0.4037977878147056 0.08702538530489344 0.050184638859155216 8 99.15004135169252 0.7066061142467462 0.06674308265985694 0.07660945140087927 9 96.62341505017014 1.8217313990491024 0.6680648745238986 0.8867886762568642 10 50.33606302958573 27.709462850313436 13.100221334563292 8.854252785537541 11 35.30271780278307 28.732301212263618 16.935139980332263 19.029841004621048 12 32.18111723189654 15.47079283026859 22.96715952043211 29.380930417402755 13 24.36391695457565 15.560138892514946 26.726366080985827 33.34957807192358 14 23.678737087608457 18.70958758669904 33.72552773644072 23.886147589251784 15 33.775422290682194 16.7811050483426 25.343242623873387 24.100230037101824 16 35.714057790657534 17.470056015339676 24.35144331601528 22.46444287798751 17 29.71858891238574 17.369686737621365 29.152343738668566 23.759380611324325 18 28.63048151145689 19.30165029139 27.6044522187122 24.46341597844091 19 35.826610622318526 20.354367368961526 20.947590412123215 22.87143159659673 20 28.9032901874554 28.17603750268332 20.84665324522369 22.074019064637593 21 27.69611895790002 19.91720985011328 30.370118963701714 22.01655222828499 22 27.71497445804941 18.404418568896546 23.574306625242585 30.306300347811455 23 29.656220719583903 19.427837100082094 22.117211590620983 28.798730589713017 24 28.52173660772567 19.214958151409217 28.126604679499266 24.136700561365846 25 33.28226377427255 17.72432884153348 22.315366133918314 26.67804125027566 26 25.438183982118495 17.455659090249355 22.99224941217452 34.11390751545763 27 23.960368446873918 17.246634714476897 22.597416923822767 36.19557991482642 28 25.390537648148744 28.020549874685262 21.61250882140254 24.976403655763463 29 34.03537426037222 19.752856794113203 22.988328702788788 23.223440242725783 30 26.988776005840315 18.98829900790246 30.89027011462493 23.132654871632294 31 30.058779025781295 25.037537100622707 22.92498399580981 21.978699877786184 32 37.96341562780086 17.797197099323718 23.323555772834677 20.915831500040742 33 29.300011933764697 18.731062425232054 25.050718499954883 26.918207141048367 34 25.059459522402484 27.644820689876013 28.293417095813528 19.002302691907975 35 24.314193764471174 24.177017955177586 32.692985394125614 18.815802886225626 36 25.199068826972553 31.638031250819438 23.07071495791366 20.09218496429435 37 26.173694899056265 24.504750721859146 29.971067360894608 19.350487018189977 38 34.05035579044948 23.129440092817436 23.130023116905075 19.69018099982801 39 27.095351699438286 20.99347289706543 22.960644894102273 28.950530509394014 40 24.644973004523564 20.973880052531737 30.891872172771052 23.489274770173648 41 22.926457624249075 21.341417137119137 30.75883448440939 24.973290754222397 42 28.093496089245207 19.95608977072671 25.219770113600198 26.730644026427886 43 21.709300355751314 18.946824233148547 32.979431849381086 26.364443561719053 44 23.359678945708175 18.516993212061735 26.272008224971664 31.851319617258422 45 23.910680791637205 20.151747994320406 31.51210419600442 24.42546701803797 46 22.81772477493277 20.404244124868466 25.64801823921431 31.13001286098445 47 22.96742499561378 26.243932393707237 28.046961810632197 22.741680800046787 48 24.412015108117835 25.872798073744235 27.557101605310663 22.158085212827274 49 24.28962939347585 25.512797639068847 26.401949113627122 23.795623853828175 50 28.450728132609104 19.92794764937254 27.410329649747144 24.210994568271214 51 22.414065517362452 26.948667532741126 26.889563802769096 23.747703147127325 52 22.68278691883096 20.825504416387165 33.37664846260558 23.115060202176295 53 23.370514873103726 19.42269586163901 33.862323909652076 23.34446535560518 54 22.744099748975696 25.477049417275545 26.466102869818382 25.312747963930377 55 22.341858517473014 26.375557104287576 25.142460570779328 26.140123807460075 56 29.446467887759404 21.031643790696585 24.300252962945613 25.221635358598398 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 7.5 4 47.5 5 80.0 6 70.0 7 60.0 8 332.0 9 604.0 10 505.5 11 407.0 12 340.5 13 343.5 14 413.0 15 649.0 16 885.0 17 1114.5 18 1344.0 19 1405.5 20 1467.0 21 1778.0 22 2513.5 23 2938.0 24 3327.0 25 3716.0 26 4251.5 27 4787.0 28 5175.5 29 5624.0 30 5684.0 31 5120.0 32 4556.0 33 5036.5 34 5517.0 35 4805.0 36 4093.0 37 3796.0 38 4023.0 39 4547.0 40 4911.5 41 5276.0 42 6356.5 43 7437.0 44 17371.5 45 27306.0 46 18348.5 47 9534.5 48 9678.0 49 11252.5 50 12827.0 51 13899.0 52 14971.0 53 16391.0 54 18688.5 55 19566.0 56 22784.0 57 26002.0 58 28539.5 59 31077.0 60 31338.0 61 31599.0 62 26790.0 63 19037.0 64 16093.0 65 14243.0 66 12393.0 67 10709.0 68 9025.0 69 7826.5 70 6628.0 71 5691.5 72 4195.5 73 3636.0 74 2850.5 75 2065.0 76 1641.0 77 1217.0 78 956.0 79 567.0 80 439.0 81 304.0 82 169.0 83 133.0 84 97.0 85 63.0 86 29.0 87 24.0 88 11.5 89 4.0 90 3.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03539032335732333 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0014504230884148906 21 0.0 22 0.0 23 0.0 24 0.009282707765855299 25 0.028718377150614836 26 0.06875005439086582 27 0.07368149289147645 28 0.11429333936709338 29 0.1842037322286911 30 0.2639770020915101 31 0.30952028706773765 32 0.31416164095066534 33 0.33765849498298656 34 0.3527428951025014 35 0.397996095461046 36 0.4362872649951991 37 0.4397682804073948 38 0.489662834648867 39 0.5363664580958266 40 0.6025057509275455 41 0.62339184340072 42 0.6410870050793817 43 0.6825691054080475 44 0.6831492746434135 45 0.7104172287056134 46 0.756250598299524 47 0.7968624447751409 48 0.848207422105028 49 0.8203592988074622 50 0.8792464761971067 51 0.8572000452532003 52 0.8833076608446684 53 0.8905597762867429 54 1.658123674675903 55 0.9366832304983363 56 0.9210186611434557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 344727.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.74410712228322 #Duplication Level Percentage of deduplicated Percentage of total 1 68.42327069520064 24.457287173862337 2 12.671103611812939 9.05834569716383 3 6.034744519246633 6.471196636546894 4 3.398583186760525 4.859172859662356 5 2.1981201175131275 3.928492047401751 6 1.5055042964296526 3.228774410678348 7 1.0718516708454604 2.681866665932887 8 0.7638243426221779 2.184177530023575 9 0.581142661789825 1.869518300070988 >10 3.03501700768246 19.681965631637517 >50 0.18347500268347444 4.55314741704079 >100 0.12447202825222495 8.344162671254574 >500 0.004849559542294478 1.3046963030048355 >1k 0.0032330396948629853 1.7802344152160754 >5k 0.0 0.0 >10k+ 8.082599237157463E-4 5.596962240503251 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 19373 5.619809298372335 No Hit TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2257 0.6547209821104816 No Hit TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1713 0.49691495009094155 No Hit TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1115 0.3234443487165206 No Hit TACTTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 1077 0.31242113324456744 No Hit TACTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 848 0.24599175579516544 No Hit TACTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 842 0.2442512480890676 No Hit TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 828 0.2401900634415059 No Hit TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 771 0.22365524023357614 No Hit TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 680 0.1972575400244251 No Hit TACTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 547 0.15867628587258903 No Hit TACTTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 493 0.14301171651770822 No Hit TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 480 0.13924061648782948 No Hit TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 479 0.13895053187014653 No Hit TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 470 0.13633977031099972 No Hit TACTTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 465 0.13488934722258483 No Hit TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG 453 0.13140833181038908 No Hit TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 434 0.12589672407441252 No Hit TACTTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 430 0.1247363856036806 No Hit TACTTCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 425 0.1232859625152657 No Hit TACTTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 425 0.1232859625152657 No Hit TACTTCGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT 400 0.11603384707319125 No Hit TACTTCGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTT 395 0.11458342398477638 No Hit TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 394 0.11429333936709338 No Hit TACTTCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 390 0.11313300089636147 No Hit TACTTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 382 0.11081232395489765 No Hit TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 377 0.10936190086648276 No Hit TACTTCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA 358 0.10385029313050617 No Hit TACTTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 356 0.1032701238951402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.9008461768297814E-4 0.0 0.0 0.0 0.0 8 5.801692353659563E-4 0.0 0.0 0.0 0.0 9 0.0011603384707319126 0.0 0.0 0.0 0.0 10 0.00464135388292765 0.0 0.0 0.0 0.0 11 0.00783228467744041 0.0 0.0 0.0 0.0 12 0.009572792383538278 0.0 0.0 0.0 0.0 13 0.011023215471953169 0.0 0.0 0.0 0.0 14 0.01566456935488082 0.0 0.0 0.0 0.0 15 0.0185654155317106 0.0 0.0 0.0 0.0 16 0.019725754002442513 0.0 0.0 0.0 0.0 17 0.020596007855491448 0.0 0.0 0.0 0.0 18 0.02320676941463825 0.0 0.0 0.0 0.0 19 0.024657192503053142 0.0 0.0 0.0 0.0 20 0.028718377150614833 0.0 0.0 0.0 0.0 21 0.03219939256281057 0.0 0.0 0.0 0.0 22 0.03800108491647013 0.0 0.0 0.0 0.0 23 0.04467303112317863 0.0 0.0 0.0 0.0 24 0.05076480809452117 0.0 0.0 0.0 0.0 25 0.05221523118293606 0.0 0.0 0.0 0.0 26 0.05337556965366797 0.0 0.0 0.0 0.0 27 0.05772683891891265 0.0 0.0 0.0 0.0 28 0.06875005439086582 0.0 0.0 0.0 0.0 29 0.07194098518537857 0.0 0.0 0.0 0.0 30 0.07600216983294027 0.0 0.0 0.0 0.0 31 0.0783228467744041 0.0 0.0 0.0 0.0 32 0.08006335448050196 0.0 0.0 0.0 0.0 33 0.08267411603964876 0.0 0.0 0.0 0.0 34 0.08586504683416152 0.0 0.0 0.0 0.0 35 0.09746843154148065 0.0 0.0 0.0 0.0 36 0.10152961618904234 0.0 0.0 0.0 0.0 37 0.1047205469835551 0.0 0.0 0.0 0.0 38 0.11110240857258062 0.0 0.0 0.0 0.0 39 0.11429333936709338 0.0 0.0 0.0 0.0 40 0.11922477786770401 0.0 0.0 0.0 0.0 41 0.12444630098599761 0.0 0.0 2.9008461768297814E-4 0.0 42 0.12792731639819335 0.0 0.0 2.9008461768297814E-4 0.0 43 0.13227858566343803 0.0 0.0 2.9008461768297814E-4 0.0 44 0.13779019339941462 0.0 0.0 2.9008461768297814E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTTA 15 0.007986927 50.681435 49 ACCGAAC 15 0.007986927 50.681435 50 TCGTGAT 15 0.008251283 50.26571 47 TATTCCG 15 0.0082752 50.228924 42 ATGTCGT 15 0.008308767 50.177513 38 CAGTACG 20 4.116193E-4 50.15551 37 AATGATG 15 0.008342437 50.126205 34 TATGGAC 20 4.16092E-4 50.045795 32 CAATTAA 15 0.008395552 50.04579 32 GTACTAG 15 0.008395552 50.04579 32 TACGAGG 15 0.0084246285 50.00204 29 AGTCCTC 25 2.0892792E-5 49.980194 28 AATGAAC 15 0.008439195 49.98019 28 CTTATGA 15 0.008478132 49.922024 25 CAATAAC 15 0.008541687 49.82779 21 CGGGATA 225 0.0 49.79887 6 AGCGCAT 20 4.2728987E-4 49.7772 15 GATATAC 15 0.008576057 49.7772 10 GGACGTA 15 0.008576057 49.7772 9 CTAATCT 15 0.008576057 49.7772 13 >>END_MODULE