FastQCFastQC Report
Fri 17 Jun 2016
SRR1526862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242274
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT194968.047087182281219No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT9680.39954761963727026No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.3702419574531316No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT6260.25838513418691234No Hit
ACAATAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT5420.22371364653243847No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.2022503446510975No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT4380.18078704276975655No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.1622130315262884No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAG2910.1201119393744273No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAGCCATGCAT2850.11763540454196489No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC2790.11515886970950245No Hit
ACAATAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA2730.11268233487704005No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTACGCCATGCAT2690.11103131165539842No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.10112517232554875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTA258.567693E-658.0924348
GTAAGCC351.6294507E-858.02028752
TAAGCCT258.631059E-658.02028753
GACAATA202.0057651E-458.00827847
GAGACTA150.004680114358.00827849
ATCCATG150.004680114358.00827855
ACTCGAG150.00468395157.99627746
ATAGACA202.0098736E-457.98427644
TATTGGT258.67352E-657.97228643
TATAGAC202.0119302E-457.97228643
ACGTACT453.092282E-1157.97228241
TACGTAC453.092282E-1157.97228240
TACGCTT650.057.9483235
TAGACCG150.00470316957.9363434
GTAATAT150.00470316957.9363433
ATAAGCT150.00470316957.9363434
TCGCATA351.6481863E-857.93633730
TCTATAC202.0181105E-457.93633331
TGCATGA202.0201739E-457.92436225
TAGTCAG150.004710872757.912411
Produced by FastQC (version 0.11.5)