FastQCFastQC Report
Fri 17 Jun 2016
SRR1526861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341587
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4813114.090407421828113No Hit
ACAATAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT17640.5164130953461344No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.4356137675028617No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT13350.39082283576365606No Hit
ACAATAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG13070.3826258025041937No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC12880.3770635299352727No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7430.21751413256359287No Hit
ACAATAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC7030.2058040850500751No Hit
ACAATAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG6890.20170556842034384No Hit
ACAATAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA6730.19702154941493677No Hit
ACAATAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4930.1443263356041067No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.12295549889193676No Hit
ACAATAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT4200.12295549889193676No Hit
ACAATAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT4160.12178449414058498No Hit
ACAATAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT4130.12090624057707114No Hit
ACAATAGGGTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC4020.11768597751085375No Hit
ACAATAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG3920.11475846563247431No Hit
ACAATAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT3810.1115382025662569No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3600.10539042762166008No Hit
ACAATAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCG3540.10363392049463241No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3470.1015846621797668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA150.004631522658.16654257
CAAATAG150.004631522658.16654258
GATCTAC201.9887426E-458.1152450
ATTATGG150.004650438758.106746
TAGGACG150.004658562658.08109344
CCCGATT150.004658562658.08109342
ATAGATG150.004677560658.02142735
TTGCGAT150.004677560658.02142735
TACGAGG150.004682999658.00440230
GTAGCGT407.166818E-1058.004429
GCCGTAT202.010517E-457.98738528
CAATAGA1450.057.9024622
GGTGTAA150.0047157357.902468
ATTCCGC150.00472393957.87703310
ACGTGAT150.00472393957.87703310
GATGCAT150.00472393957.8770339
ACCACCG258.766176E-657.87703316
GGTCTAA303.809273E-757.8770339
CACCGAA303.809273E-757.87703318
AGGGTAC61700.057.2924736
Produced by FastQC (version 0.11.5)