FastQCFastQC Report
Fri 17 Jun 2016
SRR1526860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339161
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4507713.290738027072688No Hit
ACAATAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT16220.4782389484640038No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.4289997965567975No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT11860.3496864321074652No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC11800.34791736078145774No Hit
ACAATAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG11440.3373029328254133No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6890.2031483572698512No Hit
ACAATAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA6860.2022638216068475No Hit
ACAATAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC6660.19636691718682278No Hit
ACAATAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG6520.1922390840928055No Hit
ACAATAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG5030.14830714616362142No Hit
ACAATAGGGTACCTGGTTGATCCTGNCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4380.1291422067985411No Hit
ACAATAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT3790.11174633875946823No Hit
ACAATAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT3700.1090927317704571No Hit
ACAATAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT3670.10820819610745339No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.10555458911844226No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.10525974389744104No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3570.10525974389744104No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCNCAAAGATTAAGCCATGCAT3560.10496489867643981No Hit
ACAATAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT3440.10142675602442498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAT351.549597E-858.40277541
CATTCAC201.9536934E-458.32444424
CCATTCA201.9536934E-458.32444423
AATCGTT150.00458473658.31575422
TAATCGT150.004587441758.30706421
ATCGTCG201.9870288E-458.12520657
CGCATTC150.004655474358.09069451
AACGCGC303.7133577E-758.09069450
ATGCTCG150.00470492157.935936
GCTACGC202.020815E-457.92732635
ACTACCG150.004721489757.88448334
CAGGTAA150.004729790657.8588130
CTTGGTA150.004729790657.8588131
TAGCACA150.004729790657.8588130
AACTGCC150.00477145757.73078519
CGAGATA202.0550564E-457.73078519
AATCCTA258.929621E-657.69674313
GTCGATC150.004782614357.69674316
CGTCTAA150.004782614357.69674311
GCGTCTA150.004782614357.69674310
Produced by FastQC (version 0.11.5)