FastQCFastQC Report
Fri 17 Jun 2016
SRR1526859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307585
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5055816.437082432498336No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT15910.5172553928182454No Hit
ACAATAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC13920.4525578295430531No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC13690.4450802217273274No Hit
ACAATAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC10400.33811791862412016No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA8080.262691613700278No Hit
ACAATAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA6730.21880130695580083No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA6350.20644699839068875Illumina Single End Adapter 1 (95% over 21bp)
ACAATAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG5740.18661508200985094No Hit
ACAATAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT5340.173610546678154No Hit
ACAATAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC4590.14922704293122227No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.13817318789927988No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG4220.1371978477494026No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC4210.1368727343661102No Hit
ACAATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4140.13459694068306322No Hit
ACAATAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC3970.12907001316709202No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC3810.12386819903441326No Hit
ACAATAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3450.11216411723588601No Hit
ACAATAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC3430.11151389046930116No Hit
ACAATAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA3340.10858787001966935No Hit
ACAATAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC3300.10728741648649966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGT150.00800856250.64545450
GATTACG150.00800856250.64545450
ATTACAC150.00801379650.6370849
AGATTAC150.00801379650.6370849
AACGATT150.0082734450.23032847
TGCGAAA150.00830028250.18918644
ATTGGTC252.037809E-550.18918644
GGTTATG150.00831103750.17275243
GTGCGAA150.00831103750.17275243
ATCGATA150.00831103750.17275243
TACGCTT204.135149E-450.10711335
TTCACTC204.1384876E-450.09892334
ATATGCA204.1384876E-450.09892334
ATACGCT204.1384876E-450.09892334
CGTTAGT150.00835956250.0989234
ACGTTAG150.00837578350.07435633
GTTAAAG204.165272E-450.03347832
CACGTTA150.00840287250.03347432
CAGTATA150.00841915650.00897630
GCTTAGG150.00842458950.00081629
Produced by FastQC (version 0.11.5)