##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307585
Sequences flagged as poor quality	0
Sequence length	56
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.57535640554644	39.0	38.0	40.0	36.0	40.0
2	38.47460051693028	39.0	39.0	40.0	35.0	40.0
3	38.20346245753206	39.0	38.0	40.0	35.0	40.0
4	38.48554058227807	39.0	39.0	40.0	35.0	40.0
5	38.43719947331632	39.0	39.0	40.0	35.0	40.0
6	38.382069996911426	39.0	38.0	40.0	35.0	40.0
7	38.6153258448884	39.0	38.0	40.0	36.0	40.0
8	38.664681307606024	39.0	39.0	40.0	37.0	40.0
9	38.66734723734903	39.0	39.0	40.0	36.0	40.0
10	38.01674333923956	39.0	38.0	40.0	35.0	40.0
11	38.07800770518718	39.0	38.0	40.0	35.0	40.0
12	37.99345546759432	39.0	38.0	40.0	35.0	40.0
13	37.83740429474779	39.0	38.0	40.0	35.0	40.0
14	37.957894565729795	39.0	38.0	40.0	35.0	40.0
15	37.88459450233269	39.0	38.0	40.0	34.0	40.0
16	37.79944080498074	39.0	38.0	40.0	34.0	40.0
17	37.67357315863907	39.0	38.0	40.0	33.0	40.0
18	37.72085764910513	39.0	38.0	40.0	33.0	40.0
19	37.499156330770354	39.0	37.0	40.0	33.0	40.0
20	37.423977112017816	39.0	37.0	40.0	33.0	40.0
21	37.419305232699905	39.0	38.0	40.0	33.0	40.0
22	37.397259294178845	39.0	37.0	40.0	33.0	40.0
23	37.354471121803726	39.0	37.0	40.0	33.0	40.0
24	37.08166523074922	39.0	36.0	40.0	33.0	40.0
25	36.88863241055318	39.0	36.0	40.0	32.0	40.0
26	36.677318464814604	39.0	36.0	40.0	32.0	40.0
27	36.63048588195133	39.0	36.0	40.0	31.0	40.0
28	36.093297787603426	38.0	35.0	40.0	30.0	40.0
29	36.173035095989725	38.0	35.0	40.0	30.0	40.0
30	35.70404928718891	38.0	35.0	40.0	29.0	40.0
31	35.29207861241608	38.0	35.0	40.0	28.0	40.0
32	35.12897573028594	38.0	35.0	40.0	28.0	40.0
33	34.9994278004454	38.0	34.0	40.0	27.0	40.0
34	35.14076109043029	38.0	35.0	40.0	28.0	40.0
35	34.87510119154055	38.0	34.0	40.0	27.0	40.0
36	34.52511988556009	38.0	34.0	40.0	26.0	40.0
37	34.127164848740996	38.0	33.0	40.0	23.0	40.0
38	34.23827884974885	38.0	34.0	40.0	23.0	40.0
39	34.07932441438952	38.0	33.0	40.0	23.0	40.0
40	33.66373197652681	38.0	33.0	40.0	20.0	40.0
41	33.30529772258075	38.0	33.0	40.0	15.0	40.0
42	33.04036607766959	38.0	33.0	39.0	11.0	40.0
43	32.683157501178535	37.0	32.0	39.0	10.0	40.0
44	32.78482045613408	38.0	32.0	40.0	4.0	40.0
45	32.30282035860006	37.0	32.0	39.0	4.0	40.0
46	32.013176845424844	37.0	32.0	39.0	4.0	40.0
47	31.53797486873547	36.0	31.0	39.0	4.0	40.0
48	31.391130906903783	36.0	31.0	39.0	4.0	40.0
49	31.06437570102573	36.0	30.0	39.0	4.0	40.0
50	30.83091828275111	36.0	30.0	39.0	4.0	40.0
51	29.76580132321147	35.0	29.0	39.0	4.0	40.0
52	29.659957410146788	35.0	29.0	39.0	4.0	40.0
53	29.407058211551277	35.0	28.0	39.0	4.0	40.0
54	28.92918705398508	35.0	26.0	39.0	4.0	40.0
55	28.643835037469316	35.0	25.0	39.0	4.0	40.0
56	17.1480988994912	20.0	4.0	24.0	4.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.0081806386287795
1	2	-0.8683770582320847
1	3	-0.8331408344967173
1	4	-1.1224196110392413
1	5	-1.3318490769318032
1	6	-1.0813620791495424
1	7	-0.9168788858475168
1	8	-1.1551429147483177
1	9	-1.1015306448123496
1	10	-0.9317363003729469
1	11	-1.1631454514374084
1	12	-1.4814090828898827
1	13	-1.264603987631645
1	14	-1.2875508033436986
1	15	-1.1740494757430042
1	16	-0.7029712811750244
1	17	-0.8370652086344563
1	18	-0.7364038483886688
1	19	-0.6837018560246477
1	20	-0.6884802351974457
1	21	-0.6117789941216998
1	22	-0.548384318431026
1	23	-0.45471924444468925
1	24	-0.8612085540179919
1	25	-1.0754635624823905
1	26	-0.8421425279019914
1	27	-1.2271062758798834
1	28	-0.5013916806690233
1	29	-0.7110685536865873
1	30	-0.455586753340647
1	31	-0.7259531365608538
1	32	-0.6830420317122332
1	33	-0.549845393618348
1	34	-1.220425737526611
1	35	-1.2937884481144764
1	36	-0.5361863994472671
1	37	-0.8847181065129206
1	38	0.05653647074385049
1	39	-0.51165743715476
1	40	-0.7323931902814849
1	41	-0.07555354744781084
1	42	0.06894319216004163
1	43	0.48183094081894495
1	44	-0.045558874437830355
1	45	-0.08213533437626808
1	46	0.191215920719344
1	47	0.48024113492266807
1	48	0.22977551023729603
1	49	0.6334819714142874
1	50	0.4820405795984293
1	51	1.284750101166825
1	52	1.617221287129663
1	53	0.6080438877956205
1	54	2.07357200185184
1	55	1.1026744538677207
1	56	1.4996682209468126
2	1	-0.09496757183209326
2	2	-0.27367934979717035
2	3	-0.29687803586190853
2	4	-0.25740254619282865
2	5	-0.24088125635647373
2	6	-0.12864389289893552
2	7	0.025710095137370104
2	8	-0.012237015187125166
2	9	0.024554191852686813
2	10	-0.24223118091902762
2	11	-0.23752380346081026
2	12	-0.17626525061294984
2	13	0.007921609637982385
2	14	0.0687997573583985
2	15	0.04991444429600733
2	16	0.18059770956119792
2	17	0.05743747298426172
2	18	-0.014524277447669931
2	19	0.20928936776862628
2	20	0.023379833061937916
2	21	-0.07278582006026824
2	22	0.0062475684534177844
2	23	-0.15502397384498323
2	24	-0.3165474131111168
2	25	-0.30470539573446587
2	26	-0.19718884677083537
2	27	0.1389834364555611
2	28	-0.0209918756958416
2	29	-0.21067850005420752
2	30	-0.16102312584186507
2	31	0.043915220338220706
2	32	0.14903987955056408
2	33	-0.13184198064906383
2	34	0.1971388651062469
2	35	0.1267625026412489
2	36	0.18759468295156267
2	37	0.3436461060663092
2	38	0.5293667974137648
2	39	0.03271116352783565
2	40	0.24600758982577986
2	41	-0.030697379724749396
2	42	-0.4406984460652126
2	43	-0.2154386837544351
2	44	0.03560884862408642
2	45	0.294412690977218
2	46	0.05256404358623712
2	47	-0.28744535947031125
2	48	0.1229861391987761
2	49	0.35597100115587565
2	50	0.13783043820398788
2	51	0.5504863273979304
2	52	0.8053490296942662
2	53	0.44397757867811904
2	54	0.502094673719224
2	55	0.5168870330973654
2	56	0.34089201421838666
3	1	-0.3749112234801686
3	2	-0.5419868377143473
3	3	-0.36585895530208745
3	4	-0.5193994768110528
3	5	-0.6803869484562526
3	6	-0.6898711874908656
3	7	-0.26659125401626227
3	8	-0.3010681304721885
3	9	-0.4430455057903018
3	10	-0.3523738842655675
3	11	-0.8051876374393245
3	12	-0.4717734165428098
3	13	-0.3414496252155317
3	14	-0.5992718004673776
3	15	-0.3854109330776154
3	16	-0.194725835345686
3	17	-0.24010931218191445
3	18	-0.3581919596063159
3	19	-0.41301633349348776
3	20	-0.4101245304994592
3	21	-0.8599333565378089
3	22	-0.11391643731884926
3	23	-0.3657691006287749
3	24	-0.389204718926905
3	25	-0.05223729210847239
3	26	0.20522084697816467
3	27	-0.16857319821929195
3	28	0.14616344876530007
3	29	-0.11528715387834154
3	30	0.3171361613285768
3	31	0.5214102383193051
3	32	0.2525284380864292
3	33	0.515087492287698
3	34	0.1348235433938072
3	35	0.7049172086448721
3	36	0.8077014241385427
3	37	1.0720172722023236
3	38	0.9578547833037732
3	39	0.6695506165365117
3	40	0.590809110773165
3	41	0.8050792425809519
3	42	0.8224666820929301
3	43	1.073106108604179
3	44	0.86927813419112
3	45	1.1349308209449234
3	46	1.2675582025985364
3	47	1.4449343276359947
3	48	1.4710746473417977
3	49	1.2861425658534031
3	50	1.5614509343443537
3	51	2.247190177868646
3	52	2.3454571452312933
3	53	1.9747744726470131
3	54	2.663409010480798
3	55	2.6229524979712693
3	56	1.055324980294845
4	1	-0.2768881216219725
4	2	-0.4103838609531678
4	3	-0.4088211066055578
4	4	-0.7105148491344835
4	5	-0.5408626823739766
4	6	-0.23493350772096733
4	7	0.03040002571710687
4	8	0.043496541033995584
4	9	-0.015136086989222974
4	10	-0.2006138513933564
4	11	0.05556203155578743
4	12	0.03865894432099992
4	13	-0.09011419171327617
4	14	-0.07071406864982066
4	15	-0.133573285266813
4	16	-0.14582842403216745
4	17	-0.11852779366846988
4	18	-0.16650588920499843
4	19	-0.31686512133077116
4	20	-0.1651809154695556
4	21	-0.33575858595843044
4	22	-0.352125815029666
4	23	-0.20658999274401424
4	24	-0.20338542476628874
4	25	0.08797181166726631
4	26	-0.21221055511287545
4	27	-0.3839089969683158
4	28	-0.2626161704649448
4	29	0.08893144631341698
4	30	0.6638010017613922
4	31	0.2298291763643192
4	32	0.4339647710088599
4	33	0.44692331386464446
4	34	0.6518531740380169
4	35	0.4194768580079753
4	36	0.6922149610969512
4	37	0.713071009133337
4	38	0.6427609605397677
4	39	0.9271180730493214
4	40	0.6750898029157923
4	41	0.9244464525521892
4	42	0.8262561173301108
4	43	0.8206064510230249
4	44	0.8631145949499199
4	45	0.72466738671217
4	46	1.20907306357649
4	47	1.1576220873036185
4	48	0.8459319728223313
4	49	1.0758289101897986
4	50	1.4509181306188346
4	51	1.702607244023966
4	52	1.5284457769255795
4	53	1.5842343639860985
4	54	1.7218713215797337
4	55	1.3669601681534331
4	56	0.8564709420352479
5	1	-0.15855657847840376
5	2	-0.26831440159048014
5	3	-0.3134039923914571
5	4	-0.2583845233199469
5	5	-0.2915608563804213
5	6	-0.39533451022723654
5	7	-0.18492399862947195
5	8	-0.2300802581067103
5	9	-0.17421234907300231
5	10	-0.11199945826768953
5	11	-0.26327076472061606
5	12	-0.5148677295064275
5	13	-0.190042584122871
5	14	-0.5728264925667546
5	15	-0.4007412050663106
5	16	-0.5957031107489641
5	17	-0.3219649580078894
5	18	-1.0199251516468166
5	19	-0.11872691868128982
5	20	-0.3397960246711307
5	21	-0.3797614502620519
5	22	-0.3800885790826527
5	23	-0.07332826700108086
5	24	-0.3732386292059573
5	25	-0.6435713319059531
5	26	-0.3258518010849514
5	27	-0.3773569024463015
5	28	-0.34274506412766925
5	29	-0.3955297065687873
5	30	-0.06272961048350112
5	31	0.3692849586772411
5	32	0.04678252969127783
5	33	0.46725986953954646
5	34	-0.1063279931657064
5	35	0.1496952611587048
5	36	0.002096808572780162
5	37	-0.3366604624026408
5	38	0.004092861721289864
5	39	-0.08885041961089968
5	40	0.0909150803952059
5	41	-0.042972093813730794
5	42	-0.0718462815241665
5	43	-0.23220414690035796
5	44	0.28370177719124
5	45	0.1156090265259877
5	46	-0.0460647810350423
5	47	0.08869978153920854
5	48	0.10414894382125439
5	49	-0.29703181304686765
5	50	0.5575418327312605
5	51	0.6319305522946443
5	52	0.44491552271863455
5	53	0.5356128101013873
5	54	1.1655519517265276
5	55	0.5471606694065692
5	56	0.4017985467613485
6	1	-0.23133334798838234
6	2	-0.18204700897099713
6	3	-0.5322787655312027
6	4	-0.2482816800047587
6	5	-0.14576533308943596
6	6	-0.28813547816432106
6	7	-0.07476065924653597
6	8	0.025152651754147826
6	9	-0.06507744284191119
6	10	-0.358460438303986
6	11	-0.00477106720095577
6	12	0.04248254272587815
6	13	0.1647063571959464
6	14	-0.19309267526487872
6	15	0.1390047114491182
6	16	0.2354523641451678
6	17	0.20295942190741556
6	18	0.09253703831083726
6	19	0.354352331167469
6	20	0.1451404544577244
6	21	0.10977273001623189
6	22	0.2237831692537
6	23	0.2190492284617136
6	24	0.4013530223366928
6	25	0.3561866838230259
6	26	-0.0066745476064227205
6	27	-0.20173681775180086
6	28	0.3449137380501881
6	29	-0.24604392314471113
6	30	0.42002901020615013
6	31	0.5957217402864359
6	32	0.7066131407015632
6	33	0.31825805465751955
6	34	0.5590077600103314
6	35	0.6985760839052304
6	36	0.9287150783852454
6	37	1.0993951939796887
6	38	1.046561101285718
6	39	1.0124804938797212
6	40	0.6168678934623557
6	41	0.7505548269364226
6	42	1.0025638818152132
6	43	0.9128654235775642
6	44	0.7602293029020686
6	45	0.9686035818798899
6	46	0.9248366103745198
6	47	1.069280543789663
6	48	1.1483715693505978
6	49	1.3700583260940888
6	50	1.3166464909284805
6	51	1.3533461602801253
6	52	2.1233789225976416
6	53	1.9311965971552283
6	54	2.0267739722951923
6	55	1.6383887395820054
6	56	0.30040713720297063
7	1	-0.49598551667755686
7	2	-0.6216000547477591
7	3	-0.4868864790960217
7	4	-0.20412692811241584
7	5	-0.46677939052064943
7	6	-0.41263385615302894
7	7	-0.4700147743492593
7	8	-0.3129826360026726
7	9	-0.30640869359283585
7	10	-0.21927985437991993
7	11	-0.5580931866290442
7	12	-0.3230467135519035
7	13	-0.14909876115080323
7	14	-0.5405995838315008
7	15	-0.08711567783359442
7	16	-0.4507064728126551
7	17	0.05884071471049168
7	18	-0.08039339542700219
7	19	-0.04459035776680764
7	20	-0.4030272735249767
7	21	-0.14984519621228998
7	22	-0.04193832539966991
7	23	-0.2004509517617663
7	24	-0.49291587109116364
7	25	-0.20603847537437758
7	26	-0.010783642884568678
7	27	-0.09313415044434237
7	28	0.011151873337944096
7	29	-0.038246254035016136
7	30	0.18047596442939096
7	31	-0.08135383342496283
7	32	0.11556424006477073
7	33	0.37010234157329336
7	34	0.13401677118419286
7	35	0.5750268828959761
7	36	0.5529250988105687
7	37	0.4772156913964878
7	38	0.2156828122072625
7	39	0.37161782416928446
7	40	0.27771133933524084
7	41	1.130021365444172
7	42	0.8627585231704984
7	43	0.5560469686935079
7	44	0.6533087213809807
7	45	0.9600249443693798
7	46	1.0418256768169059
7	47	1.443917093110823
7	48	1.122022896996878
7	49	1.485485455734846
7	50	1.6803855273336232
7	51	1.0405863381006242
7	52	1.5142596146557956
7	53	1.8083923198513716
7	54	1.4892514443605513
7	55	1.8042423981185927
7	56	1.663257071121027
8	1	-1.2438158521237668
8	2	-0.6603775853802105
8	3	-0.931388066969042
8	4	-0.7122720095632289
8	5	-0.9889260405585816
8	6	-0.5180120527921375
8	7	-0.6102104620204258
8	8	-0.7298925193872208
8	9	-0.9580409242008585
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113	43	1.0749343890948069
113	44	0.8360913718675889
113	45	1.1582587542936835
113	46	1.5282848862365839
113	47	1.7347977851689684
113	48	1.8104405348678334
113	49	1.8700583260940888
113	50	1.8994051116181332
113	51	2.3430013326939196
113	52	1.702689267425228
113	53	1.7346448730172952
113	54	2.0509119033296734
113	55	1.552181843030283
113	56	0.7107519647891749
114	1	0.030560281468076766
114	2	0.20977064152578606
114	3	0.042706623130399635
114	4	0.3004617448929636
114	5	0.28661339280185416
114	6	0.2967856206083255
114	7	0.3028547703268174
114	8	0.2751234290773539
114	9	0.35900204283898063
114	10	0.5729247165762033
114	11	0.4076777931146509
114	12	0.3741599243740339
114	13	0.7548875377920865
114	14	0.5330908431454091
114	15	0.5113016138453759
114	16	0.4350432466700056
114	17	0.6151745008086422
114	18	0.5164411879309441
114	19	0.5469882166145794
114	20	0.5458417987008559
114	21	0.4742379550308442
114	22	0.5326084176464576
114	23	0.5116851139088183
114	24	0.18019580433551852
114	25	0.7457834108832273
114	26	0.6304966972796038
114	27	0.6764046200038933
114	28	1.016041733374557
114	29	0.8780355625361764
114	30	0.5813381861266649
114	31	1.292448800485154
114	32	0.8457715276097986
114	33	0.9200698606189448
114	34	1.0695863690109206
114	35	1.178003319440002
114	36	0.7256174746447428
114	37	0.4393133704846619
114	38	0.6977592310344036
114	39	0.46630866454015774
114	40	0.5995680687984191
114	41	0.8832842249492785
114	42	0.6541126836854687
114	43	0.5750512798019969
114	44	0.8104338616396518
114	45	0.9520051014590862
114	46	0.9136735478379876
114	47	0.9725534835909464
114	48	0.6792307160484334
114	49	0.5874165961116233
114	50	0.5415442115596889
114	51	1.3960697137576226
114	52	1.1008774614638028
114	53	1.3032012727835145
114	54	1.5604969413191547
114	55	1.4316090785650566
114	56	1.1380459449177565
115	1	0.021814277487138156
115	2	0.18999864080198137
115	3	0.14061361390186278
115	4	-0.09163669288977871
115	5	0.19091401406972608
115	6	0.2663374633008928
115	7	0.2586422122714467
115	8	0.2453146348195574
115	9	0.17055883154758789
115	10	0.25660889535658526
115	11	0.22152663803895223
115	12	0.16091425213807753
115	13	0.24589423300892577
115	14	0.1905249465292087
115	15	0.20060736208109375
115	16	-0.034237168312799326
115	17	0.14215441515811733
115	18	0.1818854246159063
115	19	0.18534999959304344
115	20	0.2982635370445479
115	21	0.41037403078933465
115	22	0.3241881269172211
115	23	0.37139921005454823
115	24	0.3338600784171888
115	25	0.3879451817982371
115	26	0.5608061248687264
115	27	0.7075403941760428
115	28	0.7208248927931038
115	29	0.8283823868319686
115	30	1.2198326670965685
115	31	1.2898069041102218
115	32	1.3368867939105442
115	33	0.9788912611859217
115	34	1.3315442675572697
115	35	1.4232171441580164
115	36	1.5352803011119605
115	37	1.5844608462069587
115	38	1.7391368774545768
115	39	1.180513381350579
115	40	1.4815874909589795
115	41	1.6570341495526932
115	42	1.794832871875343
115	43	1.4992686632388725
115	44	1.3970313645047199
115	45	1.8533501765540876
115	46	1.539525529260267
115	47	1.4022416433110756
115	48	1.9003779505016603
115	49	0.9408154742104209
115	50	1.1108789120844484
115	51	1.7117582353813638
115	52	1.381557840256253
115	53	1.775557868476863
115	54	2.347745870687632
115	55	2.3245097360232876
115	56	1.034975795934102
116	1	-0.08132742311576635
116	2	-0.028379512822162667
116	3	-0.12910303380942878
116	4	0.015552897551820877
116	5	-0.040231502066802705
116	6	-0.16473223133412773
116	7	-0.0146943006732414
116	8	-0.03894262019750272
116	9	-0.001776103820695596
116	10	-0.24384970243480808
116	11	-0.09090686528129766
116	12	-0.06415331460318896
116	13	0.09948535944739234
116	14	-0.10977711654939526
116	15	0.0907762483610739
116	16	-0.2354209620783152
116	17	-0.22617910161432775
116	18	0.13449698617195338
116	19	-0.0471407367315706
116	20	-0.08014690186411144
116	21	-0.19777555769557864
116	22	-0.005011711656358386
116	23	-0.2604507122895612
116	24	-0.0366135613817562
116	25	0.12864787590739724
116	26	-0.0877979034542733
116	27	-0.18245728226181512
116	28	0.19576099483416698
116	29	-0.08068072845339458
116	30	-0.31908699879965496
116	31	-0.11181469768014551
116	32	0.3671297895936121
116	33	0.5440549500242682
116	34	0.3367539384674956
116	35	0.5146798876734451
116	36	-0.5708820302769197
116	37	0.7964920063982248
116	38	0.6232645021625984
116	39	0.7821569958349315
116	40	0.8772778946473281
116	41	0.6303865605541503
116	42	0.7174827110603843
116	43	-0.03741304543535051
116	44	0.21233263268047864
116	45	-0.5199851134631572
116	46	0.13494444305611353
116	47	-0.08022769178327493
116	48	-0.05258826001307426
116	49	0.42718594785021935
116	50	-0.3676525966411397
116	51	0.5214584958649091
116	52	0.457648385804184
116	53	-0.20025262668645993
116	54	0.3810339557055471
116	55	0.22533032033802058
116	56	-0.21979667841499406
117	1	-0.07613698783512746
117	2	-0.10022229632731694
117	3	-0.2706408344967173
117	4	-0.36478072215035695
117	5	-0.21667050550323097
117	6	-0.08612398391144893
117	7	0.03996635224771694
117	8	-0.06754370839910706
117	9	0.004917767886063018
117	10	-0.31576407815072827
117	11	-0.26126053080248823
117	12	-0.4920241622549639
117	13	-0.2798817654094208
117	14	-0.3510428668357619
117	15	-0.7357558249493508
117	16	-0.37201890022264905
117	17	-0.41940647847572876
117	18	-0.339479245214072
117	19	-0.34471376078655425
117	20	-0.3475080129752257
117	21	-0.11247343856614123
117	22	-0.09858273112944005
117	23	0.28526091428546607
117	24	0.20654938248994625
117	25	0.27781024704141544
117	26	0.2024011228916578
117	27	0.15166356538995984
117	28	0.2157710177436769
117	29	0.37117350980548025
117	30	-0.06550738826128111
117	31	0.3102571808994625
117	32	0.35793019050998964
117	33	0.2543212730483191
117	34	0.07064569104481677
117	35	0.5528385360125085
117	36	0.6535953465844742
117	37	0.214984274439459
117	38	0.603361867569248
117	39	0.6411203406230186
117	40	0.98645601606772
117	41	0.670478198583929
117	42	1.0404709699378216
117	43	0.5346087185967221
117	44	0.6660482684193099
117	45	0.19207101483008415
117	46	0.22593814294156545
117	47	0.2984951031766343
117	48	0.28364455785634135
117	49	0.3518351460174607
117	50	0.22380645261430132
117	51	0.7705635932303174
117	52	0.36107049347887
117	53	0.28363912589086127
117	54	0.9011513669312059
117	55	0.43491651735978465
117	56	-0.01987542984683799
118	1	-0.30818063862877665
118	2	-0.029091343946369363
118	3	-0.31171226306814503
118	4	-0.19384818246781066
118	5	-0.07113479121751709
118	6	-0.39921922200668547
118	7	-0.03473602870466408
118	8	-0.062285771891168906
118	9	-0.022959216240920455
118	10	-0.24602201465866358
118	11	-0.09171688000883904
118	12	-0.3528376543184564
118	13	-0.0038897019173589342
118	14	-0.20540794620084313
118	15	-0.3311923328858555
118	16	-0.3922569954607411
118	17	-0.5370652086344592
118	18	-0.17211813410295917
118	19	-0.5337018560246491
118	20	-0.2920516637688735
118	21	0.002506720164014098
118	22	-0.28052717557388007
118	23	-0.2261478158732615
118	24	0.22450573169629706
118	25	-0.08260641962525028
118	26	0.28642890066943494
118	27	-0.16996341873702647
118	28	0.40217974790240874
118	29	0.2603600177419878
118	30	0.3944132466593544
118	31	-0.45095313656085523
118	32	0.01695796828776963
118	33	-0.29984539361834806
118	34	-0.248997166098043
118	35	0.05978298045695141
118	36	0.09952788626701903
118	37	0.16885332205851
118	38	0.017250756458132344
118	39	-1.7295145800119016
118	40	0.14260680971851514
118	41	0.09230359540932653
118	42	0.2725146207314708
118	43	0.146116655104656
118	44	0.7044411255621696
118	45	0.33929323705230274
118	46	1.2340730635764885
118	47	1.2552411349226666
118	48	1.0262040816658633
118	49	1.2477676856999977
118	50	1.1284691510270015
118	51	1.0133215297382527
118	52	0.7029355728439484
118	53	0.5187581735099087
118	54	0.3450005732804122
118	55	0.12767445386771925
118	56	-0.028903207624615845
119	1	-0.07625350535936093
119	2	-0.05540965650629204
119	3	-0.2718992237584601
119	4	-0.2643659197640744
119	5	-0.23407822362403863
119	6	-0.09926716064522623
119	7	-0.024980515761228617
119	8	0.08819361449425855
119	9	-0.010159599750032555
119	10	0.010042223116982996
119	11	-0.019952738110468715
119	12	0.21279034184644985
119	13	0.20553024055627844
119	14	0.008110749868194489
119	15	0.020629335378764324
119	16	-0.10076610380206574
119	17	0.24311216432815286
119	18	-0.16356108712309236
119	19	0.19101051214409637
119	20	0.13685533527235094
119	21	0.2474491937977632
119	22	0.10420436805027578
119	23	0.22147922151887656
119	24	0.23495635489859978
119	25	0.22964190252240257
119	26	0.1783752093942681
119	27	0.42203082862634744
119	28	0.5220023749397953
119	29	0.6632363360545455
119	30	0.6862636781071032
119	31	0.4878531913394326
119	32	0.5109176998313956
119	33	0.44461769362997927
119	34	1.0073067648703216
119	35	1.0975346583093
119	36	0.9989765919237783
119	37	1.1391313661620543
119	38	1.0383916960554487
119	39	1.2299533011002737
119	40	1.4632923322496723
119	41	1.570179913721887
119	42	1.3526680243748075
119	43	1.5529718804162584
119	44	1.6612244429159517
119	45	1.59801806926707
119	46	2.287692430786457
119	47	2.1664108377031077
119	48	1.8536249829122688
119	49	1.742518404779581
119	50	1.9372898605188489
119	51	2.549203600687438
119	52	2.1273363398621647
119	53	1.763365076673857
119	54	2.109717735313012
119	55	1.8544961221323426
119	56	1.0150565239190072
120	1	-0.45246144373432173
120	2	-0.554152708698453
120	3	-0.5586562984142418
120	4	-0.56863954231072
120	5	-0.6725339075650894
120	6	-0.6011583481726177
120	7	-0.6985430979241016
120	8	-0.5406608332559202
120	9	-0.7784084062261982
120	10	-0.4981452350808553
120	11	-0.5135872776524337
120	12	-0.6964557201014685
120	13	-0.18917443436703962
120	14	-0.42592831929853503
120	15	-0.5071618959688209
120	16	-0.7405756012535747
120	17	-0.7244608885559103
120	18	-0.48062328874213023
120	19	-0.5007489841542494
120	20	-0.3035145994586159
120	21	-0.4864353515100177
120	22	-0.4345649762611714
120	23	-0.5834747672723779
120	24	-0.40087472976664174
120	25	-0.3455789282163124
120	26	-0.3558391405676744
120	27	-0.4092614059731545
120	28	-0.3368850532758003
120	29	-0.4037047834362184
120	30	-0.013245074401027068
120	31	-0.03277689699285702
120	32	-0.2445540592036437
120	33	-0.2566323351991002
120	34	-0.010325099333186927
120	35	-0.042021143254387994
120	36	0.04767467075400589
120	37	0.3772480201439308
120	38	0.3895875262175821
120	39	0.40415012298269914
120	40	0.26215761973323737
120	41	-0.12906361126715638
120	42	0.1023624361463007
120	43	-0.027103767084838637
120	44	0.08862129247429351
120	45	0.012660934646810063
120	46	0.5919891165956344
120	47	0.507401174196108
120	48	0.6828069289903631
120	49	0.4512409306680887
120	50	0.319632626726559
120	51	0.5248949219817476
120	52	0.746308179618616
120	53	0.26366489908673785
120	54	1.2848140244340698
120	55	0.661756437176507
120	56	0.5705813284578589
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	169.0
5	23.0
6	51.0
7	78.0
8	149.0
9	223.0
10	178.0
11	279.0
12	320.0
13	439.0
14	605.0
15	792.0
16	1006.0
17	1283.0
18	1631.0
19	1858.0
20	1918.0
21	2317.0
22	2468.0
23	2645.0
24	3513.0
25	4628.0
26	4682.0
27	4609.0
28	5246.0
29	5680.0
30	6552.0
31	8043.0
32	9776.0
33	11805.0
34	15688.0
35	21121.0
36	29057.0
37	42699.0
38	55870.0
39	60184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.72430385096803	0.22725425492140383	0.02340816359705447	0.025033730513516587
8	99.5622379851239	0.36198300338566314	0.030246558234381556	0.04553245325605826
9	98.60331290537574	0.6970430937789553	0.25001219175187345	0.4496318090934213
10	41.92142009525822	24.024578571776907	25.275939984069446	8.778061348895427
11	27.117707300421024	43.56226734073508	14.662613586488288	14.657411772355609
12	28.59599785425167	13.765950875367785	18.518133198953134	39.11991807142741
13	21.825186533803663	13.36963766113432	22.21369702683811	42.59147877822391
14	21.05369247525074	17.221906139766244	42.42241981891184	19.301981566071166
15	41.972137783051835	16.794056927353417	22.52873189524847	18.70507339434628
16	44.05676479672286	16.878911520392737	19.458686216818116	19.60563746606629
17	25.365996391241445	16.780402165255133	37.72258075003658	20.131020693466848
18	22.710795389892226	17.474519238584456	39.98862103158476	19.826064339938554
19	42.086577693970774	18.367605702488742	19.30653315343726	20.239283450103223
20	20.96591186176179	40.09688378822114	18.020059495749145	20.917144854267928
21	20.50067461027033	17.830193279906368	41.165206365720046	20.503925744103256
22	21.23413040297804	17.237836695547575	20.564071720012354	40.96396118146203
23	21.16195523188712	19.282799876456913	19.387486385877075	40.16775850577889
24	20.158020516655558	20.87561574352555	38.40711417470046	20.559249565118435
25	42.04208403798621	18.123780408481853	19.234421751008195	20.59971380252374
26	21.199904991524075	17.5814486189607	20.305458757536417	40.91318763197881
27	19.602975245334097	17.22577244449072	20.939948460317048	42.231303849858136
28	20.902148934646608	38.15221566163881	20.86243823684502	20.08319716686956
29	41.59704511476563	19.056599471690493	21.083523062240854	18.26283235130302
30	21.784669950481977	17.939606920135482	42.084777201498255	18.190945927884286
31	20.812924349249624	36.84867893271068	24.754603027595337	17.583793690444356
32	41.06505170676346	17.9612109590292	24.028072647105823	16.94566468710152
33	21.3870022769829	18.011978626372553	23.65843951641842	36.94257958022613
34	19.811216099814676	38.0259586019681	26.144906162720886	16.017919135496335
35	19.315614921592434	21.468068993432308	44.86825440348884	14.348061681486415
36	21.305651160208726	38.64282029010116	24.23343935109294	15.818089198597168
37	19.09111691704768	19.441868060091444	44.80339647289354	16.66361854996734
38	39.398404851447296	19.52668328688168	24.954174603745102	16.120737257925917
39	20.492033708820127	18.083186777983354	24.043685480233005	37.38109403296351
40	19.760202829802896	19.066001472151793	44.16684387012349	17.006951827921814
41	19.418022139110576	18.633696864989382	44.32272187374228	17.62555912215776
42	36.503182941397874	18.0918716350009	27.05221987660988	18.352725546991344
43	17.696229139450136	17.609136241450326	46.500731776793344	18.193902842306194
44	18.92571480956419	17.438635894850226	26.583745592294168	37.051903703291416
45	17.495611277052873	18.264292826075565	45.18092019074569	19.05917570612587
46	17.060176538816915	18.26298648108466	26.863519898296868	37.813317081801564
47	17.548511865740135	37.267601940474634	28.092631440933523	17.09125475285171
48	18.742722952341282	38.17846682650207	25.901533960649004	17.177276260507647
49	19.491483746168097	37.73446336945296	25.04024524188128	17.733807642497666
50	36.74805123225112	19.55591716752841	25.343822996771735	18.352208603448727
51	18.35292960102327	39.09939161378134	25.592561617553006	16.95511716764238
52	18.48986782406724	21.100252279206487	42.85957988458801	17.55030001213827
53	19.518036713200676	18.45929231122558	44.37080660771994	17.651864367853804
54	19.289711095825176	37.6746124133552	24.498320016932112	18.537356473887506
55	19.742068050969298	37.884944888299515	23.960295942282066	18.412691118449125
56	36.79030416004831	19.711986557622378	23.0318862648831	20.465823017446212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	4.5
4	15.0
5	22.0
6	173.0
7	324.0
8	367.0
9	410.0
10	415.5
11	421.0
12	468.5
13	933.5
14	1351.0
15	1592.5
16	1834.0
17	2143.0
18	2452.0
19	2884.0
20	3316.0
21	4001.0
22	5119.5
23	5553.0
24	5907.0
25	6261.0
26	6616.0
27	6971.0
28	7095.5
29	6178.0
30	5136.0
31	5399.5
32	5663.0
33	4965.0
34	4267.0
35	4021.0
36	3775.0
37	3983.0
38	4407.5
39	4624.0
40	6363.0
41	8102.0
42	34121.0
43	60140.0
44	35779.0
45	11418.0
46	9942.5
47	10242.0
48	12017.0
49	11404.0
50	10791.0
51	11293.5
52	11796.0
53	11948.5
54	13758.0
55	15415.0
56	17022.0
57	18629.0
58	17288.5
59	15948.0
60	14607.5
61	13267.0
62	12046.0
63	9740.5
64	8656.0
65	7488.0
66	6320.0
67	5669.0
68	5018.0
69	4326.0
70	3634.0
71	3069.5
72	1972.0
73	1439.0
74	1158.5
75	878.0
76	746.5
77	615.0
78	460.5
79	237.5
80	169.0
81	122.5
82	76.0
83	55.5
84	35.0
85	23.5
86	12.0
87	6.5
88	1.0
89	1.0
90	1.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0364126989287514
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.009753401498772697
25	0.03413690524570444
26	0.07932766552335127
27	0.08095323243981338
28	0.11801615813514964
29	0.1846644017100964
30	0.26919388136612643
31	0.3052814669115854
32	0.30950794089438693
33	0.3377928052408277
34	0.3566493814717883
35	0.4021652551327275
36	0.4379277272948941
37	0.45028203586000615
38	0.49807370320399236
39	0.5432644634816393
40	0.6209665620885284
41	0.6411235918526585
42	0.6664824357494676
43	0.703545361444804
44	0.7002942276118796
45	0.7337809060909992
46	0.7747451923858445
47	0.8144090251475201
48	0.8739047742900337
49	0.8387925288944519
50	0.9028398654030594
51	0.8713038672236942
52	0.8986133914202579
53	0.9070663393858609
54	1.6909147065038932
55	0.9529073264300926
56	0.9353512037323016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	307585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.60355703702013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.44799992326087	22.64251826605005
2	12.6729999859965	8.263697557471843
3	5.906999993472842	5.77767633614608
4	3.312000105938255	4.319319374422985
5	2.000999998681464	3.2619858794044156
6	1.3509999985152834	2.642844330516429
7	0.9459999989547588	2.159007544605972
8	0.7219999992022001	1.883181452377394
9	0.4989999994486115	1.4642257449146237
>10	2.796999996909351	16.524460794813596
>50	0.21499999976242778	4.838693894517464
>100	0.11899999986850654	6.929560004991185
>500	0.004999999994475065	1.0511386777120344
>1k	0.003999999995580051	1.7579837934935763
>5k	0.0	0.0
>10k+	9.999999988950128E-4	16.483706348562357
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	50558	16.437082432498336	No Hit
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1591	0.5172553928182454	No Hit
ACAATAGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	1392	0.4525578295430531	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	1369	0.4450802217273274	No Hit
ACAATAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	1040	0.33811791862412016	No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	808	0.262691613700278	No Hit
ACAATAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	673	0.21880130695580083	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA	635	0.20644699839068875	Illumina Single End Adapter 1 (95% over 21bp)
ACAATAGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	574	0.18661508200985094	No Hit
ACAATAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	534	0.173610546678154	No Hit
ACAATAGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC	459	0.14922704293122227	No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.13817318789927988	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG	422	0.1371978477494026	No Hit
ACAATAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	421	0.1368727343661102	No Hit
ACAATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	414	0.13459694068306322	No Hit
ACAATAGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC	397	0.12907001316709202	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	381	0.12386819903441326	No Hit
ACAATAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	345	0.11216411723588601	No Hit
ACAATAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC	343	0.11151389046930116	No Hit
ACAATAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA	334	0.10858787001966935	No Hit
ACAATAGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC	330	0.10728741648649966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0052018141326787715	0.0	0.0	0.0	0.0
7	0.0052018141326787715	0.0	0.0	0.0	0.0
8	0.006177154282556042	0.0	0.0	0.0	0.0
9	0.006502267665848465	0.0	0.0	0.0	0.0
10	0.010728741648649967	0.0	0.0	0.0	0.0
11	0.017556122697790853	0.0	0.0	0.0	0.0
12	0.01885657623096055	0.0	0.0	0.0	0.0
13	0.01918168961425297	0.0	0.0	0.0	0.0
14	0.022757936830469627	0.0	0.0	0.0	0.0
15	0.02535884389680901	0.0	0.0	0.0	0.0
16	0.02600907066339386	0.0	0.0	0.0	0.0
17	0.027959750963148396	0.0	0.0	0.0	0.0
18	0.029585317879610513	0.0	0.0	0.0	0.0
19	0.03381179186241202	0.0	0.0	0.0	0.0
20	0.040639172911552904	0.0	0.0	0.0	0.0
21	0.04324007997789229	0.0	0.0	0.0	0.0
22	0.048767007493863486	0.0	0.0	0.0	0.0
23	0.053968821626542256	0.0	0.0	0.0	0.0
24	0.058520408992636185	0.0	0.0	0.0	0.0
25	0.0601459759090983	0.0	0.0	0.0	0.0
26	0.06534779004177707	0.0	0.0	0.0	0.0
27	0.06892403725799373	0.0	0.0	0.0	0.0
28	0.08062811905652097	0.0	0.0	0.0	0.0
29	0.08290391273956793	0.0	0.0	0.0	0.0
30	0.08973129378870881	0.0	0.0	0.0	0.0
31	0.09363265438821788	0.0	0.0	0.0	0.0
32	0.09493310792138758	0.0	0.0	0.0	0.0
33	0.09850935513760424	0.0	0.0	0.0	0.0
34	0.1020856023538209	0.0	0.0	0.0	0.0
35	0.11411479753564055	0.0	0.0	0.0	0.0
36	0.1202919518181966	0.0	0.0	0.0	0.0
37	0.12354308565112083	0.0	0.0	0.0	0.0
38	0.12646910610075263	0.0	0.0	0.0	0.0
39	0.1300453533169693	0.0	0.0	0.0	0.0
40	0.1371978477494026	0.0	0.0	0.0	0.0
41	0.14337500203195866	0.0	0.0	0.0	0.0
42	0.14922704293122227	0.0	0.0	0.0	0.0
43	0.1541037436806086	0.0	0.0	0.0	0.0
44	0.15735487751353283	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGT	15	0.008008562	50.645454	50
GATTACG	15	0.008008562	50.645454	50
ATTACAC	15	0.008013796	50.63708	49
AGATTAC	15	0.008013796	50.63708	49
AACGATT	15	0.00827344	50.230328	47
TGCGAAA	15	0.008300282	50.189186	44
ATTGGTC	25	2.037809E-5	50.189186	44
GGTTATG	15	0.008311037	50.172752	43
GTGCGAA	15	0.008311037	50.172752	43
ATCGATA	15	0.008311037	50.172752	43
TACGCTT	20	4.135149E-4	50.107113	35
TTCACTC	20	4.1384876E-4	50.098923	34
ATATGCA	20	4.1384876E-4	50.098923	34
ATACGCT	20	4.1384876E-4	50.098923	34
CGTTAGT	15	0.008359562	50.09892	34
ACGTTAG	15	0.008375783	50.074356	33
GTTAAAG	20	4.165272E-4	50.033478	32
CACGTTA	15	0.008402872	50.033474	32
CAGTATA	15	0.008419156	50.008976	30
GCTTAGG	15	0.008424589	50.000816	29
>>END_MODULE