FastQCFastQC Report
Fri 17 Jun 2016
SRR1526858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105992
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT41603.9248245150577397No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT2040.19246735602686996No Hit
AGCGAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT2020.19058042116386142No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1960.18491961657483583No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT1430.13491584270510984No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1250.11793342893803306No Hit
AGCGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG1130.1066118197599819No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT1060.10000754773945204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTAC150.004513251658.5193358
AAAGGTA150.004513251658.5193357
ACGATTA407.039489E-1058.048348
GTTTTTA201.9937646E-458.048350
TAGCTTT201.9937646E-458.048348
AGCTTAA501.8189894E-1258.04829849
CAAGATT258.573035E-658.04829847
TTACGCC258.573035E-658.04829852
CCTAACG150.004660095658.04829451
ATCCCTA150.004660095658.04829448
AATGTGA150.004660095658.04829449
AAGCTTA550.058.04829448
AAATGTG150.004660095658.04829448
TATCCCT150.004660095658.04829447
GATAAAG258.597148E-658.02082446
CAACGAT453.092282E-1158.02082446
ATAAGTG258.621317E-657.99337840
TTAACGC202.0031228E-457.99337813
CTCAACG550.057.99337444
CTATCAA150.004677602657.99337439
Produced by FastQC (version 0.11.5)