FastQCFastQC Report
Fri 17 Jun 2016
SRR1526857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161362
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT107196.642827927269121No Hit
AGCGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG13110.8124589432456216No Hit
AGCGAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT10330.6401755060051313No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC9060.5614704825175691No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC7070.4381452882339088No Hit
AGCGAGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG6110.37865172717244455No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6010.3724544812285421No Hit
AGCGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT4740.2937494577409799No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4680.2900311101746384No Hit
AGCGAGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4500.27887606747561383No Hit
AGCGAGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2920.18095958156195388No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2870.1778609585900026No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2810.17414261102366108No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2570.159269220758295No Hit
AGCGAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG2430.15059307643683148No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2310.14315638130414846No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT2290.14191693211536793No Hit
AGCGAGGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT2290.14191693211536793No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCA2210.1369591353602459No Hit
AGCGAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA2200.13633941076585565No Hit
AGCGAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC2180.13509996157707516No Hit
AGCGAGGGGGGGCTGGCAAGATGGCTCAGTGGTTAAGAGTGCTGACTGCTCTTCCGAAGGTCCC2120.13138161401073364No Hit
AGCGAGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT1950.12084629590609934No Hit
AGCGAGGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG1820.11278987617902604No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1820.11278987617902604No Hit
AGCGAGGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT1810.1121701515846358No Hit
AGCGAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC1810.1121701515846358No Hit
AGCGAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC1710.10597290564073325No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGTGCCAACTGCTCTTCCAAAGGTCC1660.102874282668782No Hit
AGCGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1650.10225455807439175No Hit
AGCGAGGGGGACCTGAGCATCGCGCACCCAGCCATGACAGCCCATTCCTTTGCCCTCCCTGTCA1650.10225455807439175No Hit
AGCGAGGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT1630.10101510888561123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAA258.439476E-658.2254557
GCCTACT303.642217E-758.2254453
AAGGTTC150.00461426358.20729458
TAGCAAT201.9792162E-458.15291652
AGGGCGA201.9792162E-458.15291652
TTTCGGG150.00463137758.15291252
CCACTCG150.004637092458.13480851
TCACGAA150.00464281358.1167144
CCTAGTT150.00464281358.1167142
TTCACGA150.00464281358.1167143
TAAGTGG150.00464281358.1167143
TATCTCA150.00464281358.1167148
ACAATTA150.00464281358.1167143
ACCAATA150.00464281358.1167148
TCGAGTT150.00464281358.1167149
ATTATCT150.00464281358.1167146
TAGTCAA150.00464281358.1167145
ACCTGTC150.00464853958.0986341
TCCTAGT150.00464853958.0986341
GCTAAGT201.9883788E-458.0986341
Produced by FastQC (version 0.11.5)