FastQCFastQC Report
Fri 17 Jun 2016
SRR1526856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158993
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT98306.182662129779298No Hit
AGCGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG11320.7119810306114105No Hit
AGCGAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT9310.5855603705823528No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7710.4849270093651921No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC7440.46794512965979634No Hit
AGCGAGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG5110.32139779738730634No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4870.3063027932047323No Hit
AGCGAGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG4470.28114445290044215No Hit
AGCGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT4390.2761127848395841No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4310.2710811167787261No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2770.17422150660720911No Hit
AGCGAGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC2600.16352921197788584No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2490.15661066839420604No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2290.14403149824206096No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT2280.14340253973445372No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC2160.1358550376431667No Hit
AGCGAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG2150.13522607913555942No Hit
AGCGAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA2030.12767857704427238No Hit
AGCGAGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT1980.12453378450623612No Hit
AGCGAGGGGGGGCTGGCAAGATGGCTCAGTGGTTAAGAGTGCTGACTGCTCTTCCGAAGGTCCC1890.11887315793777085No Hit
AGCGAGGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT1870.11761524092255633No Hit
AGCGAGGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT1770.11132565584648381No Hit
AGCGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1760.11069669733887655No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1720.10818086330844753No Hit
AGCGAGGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT1700.10692294629323304No Hit
AGCGAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTC1700.10692294629323304No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCA1690.1062939877856258No Hit
AGCGAGGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGG1610.10126231972476776No Hit
AGCGAGGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTC1610.10126231972476776No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.10063336121716049No Hit
AGCGAGGGGGACCTGAGCATCGCGCACCCAGCCATGACAGCCCATTCCTTTGCCCTCCCTGTCA1600.10063336121716049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATC150.004534438358.4638445
TATTACC201.9275947E-458.46383745
CGCCAAA201.930634E-458.4452243
TATCACA150.00455162858.4080239
GCAGTAG150.00455162858.4080226
GAATTTA150.00455162858.4080241
TGCAGTA150.00455162858.4080226
AATACTA201.936724E-458.4080226
TACACCT150.00455162858.4080226
CGCTTTC258.283545E-658.4080239
TACATCA150.004563114658.3708624
CATAGAA452.910383E-1158.3708622
TCTAATG150.004563114658.3708621
TTACGGA150.004563114658.3708621
CTAATGC150.004563114658.3708622
GTTACGG150.004574622558.33375520
TCGAGTT201.998469E-458.0385849
TCGCAAG258.617384E-658.02023347
AAGCATA150.00467331758.02023347
CGTAACC150.00467331758.02023357
Produced by FastQC (version 0.11.5)