FastQCFastQC Report
Fri 17 Jun 2016
SRR1526855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143712
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG109757.636801380538856No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13260.9226786907147628No Hit
AGCGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC10310.7174070362948118No Hit
AGCGAGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC8340.5803273213092852No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC5980.4161099977733244No Hit
AGCGAGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT4300.299209530171454No Hit
AGCGAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC4190.29155533288799823No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3990.2776386105544422No Hit
AGCGAGGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC3820.2658093965709196No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3600.250501002004008No Hit
AGCGAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC3140.2184925406368292No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2540.17674237363616122No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2360.1642173235359608No Hit
AGCGAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT2240.15586729013582723No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2190.15238810955243823No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2030.1412547316855934No Hit
AGCGAGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG2010.13986305945223781No Hit
AGCGAGGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC1960.1363838788688488No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA1910.1329046982854598Illumina Single End Adapter 1 (95% over 21bp)
AGCGAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCC1890.13151302605210421No Hit
AGCGAGGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA1750.121771320418615No Hit
AGCGAGGGGGGGCTGGCAAGATGGCTCAGTGGTTAAGAGTGCTGACTGCTCTTCCG1750.121771320418615No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1730.12037964818525941No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1720.11968381206858161No Hit
AGCGAGGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1710.11898797595190382No Hit
AGCGAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT1700.118292139835226No Hit
AGCGAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1690.1175963037185482No Hit
AGCGAGGGGGACCTGAGCATCGCGCACCCAGCCATGACAGCCCATTCCTTTGCCCT1590.1106379425517702No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC1550.107854598085059No Hit
AGCGAGGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA1530.1064629258517034No Hit
AGCGAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1450.10089623691828102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC150.0080083450.63176750
CGATTAA150.0080083450.63176749
TGGATAG150.0080083450.63176750
AAGCATA150.00825764750.24078847
AACGATT150.00825764750.24078847
TTAGGAA150.00826911450.2231646
CCCCTTA150.00826911450.2231644
CAACGAT150.00826911450.2231646
ACTCCTA150.00826911450.2231645
TAAGCAT150.00826911450.2231646
AGTACGG150.00826911450.2231644
GTACGGA150.00826911450.2231645
AACCCCT150.008292085550.18794342
TAGTACG150.008292085550.18794343
CTAGTAC204.0949762E-450.1879442
CCGTGAT150.00831510450.15276741
GACCATA150.00831510450.15276741
TCTATAA150.00831510450.15276741
TTCTATA150.00832663150.13520440
CGCTAGT150.00832663150.13520440
Produced by FastQC (version 0.11.5)