FastQCFastQC Report
Fri 17 Jun 2016
SRR1526854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40432
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5531.3677285318559556No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4761.1772853185595569No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.49960427384250095No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.4748713889988128No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.472398100514444No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.41551246537396125No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.41551246537396125No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.3338939453897903No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.296794618124258No Hit
TTGGACGGGATGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGC1020.2522754254056193No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.23248911753066878No Hit
TTGGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.22754254056193113No Hit
TTGGACGGGACATAAGATACTAAAAGTCTCATTTTATATTTGTTTTAGATGTTTTAAATAGTTG870.21517609814008706No Hit
TTGGACGGGTGATTTGAAACCTAGGTTGATTTCTTTTCTCACTCTTGAAAGGAGTATTTCTTTG840.20775623268698062No Hit
TTGGACGGGGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA760.18796992481203006No Hit
TTGGACGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.13850415512465375No Hit
TTGGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.13603086664028494No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.13108428967154728No Hit
TTGGACGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.12366442421844084No Hit
TTGGACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.12366442421844084No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1187178472497032No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11624455876533439No Hit
TTGGACGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10882469331222792No Hit
TTGGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.10387811634349031No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA410.10140482785912149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTG150.004477136358.57089256
ACTGTTT150.004635149658.06094730
ATCCTGG150.004635149658.06094728
GGCAAGG150.004635149658.06094754
AGGGCGG150.004635149658.06094746
ACATTAA150.004635149658.06094753
GCGTGCC150.004635149658.06094740
CTTGGGA150.004635149658.06094751
CATTAAA150.004635149658.06094754
TCTTGTA150.004635149658.06094743
ATCATTT150.004635149658.06094731
AGCACGC150.004635149658.06094748
CTTGTAC150.004635149658.06094744
TACCTTG150.004635149658.06094748
AGCGCGT150.004635149658.06094737
TTGGATT150.004635149658.06094750
TTGTGTG150.004635149658.06094738
GTGTGGG150.004635149658.06094740
CGCGTGC150.004635149658.06094739
TGTGTGG150.004635149658.06094739
Produced by FastQC (version 0.11.5)