FastQCFastQC Report
Fri 17 Jun 2016
SRR1526853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51265
Sequences flagged as poor quality0
Sequence length64
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT13242.582658734029065No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.9402126206963816No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3690.7197893299522091No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3510.684677655320394No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.5578854969277285No Hit
TTGGACGGGATGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGC2770.540329659611821No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.4681556617575344No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.45059982444162683No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.40183360967521703No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.37257388081537113No Hit
TTGGACGGGATCTGGGTCCCGGCTGACGGATTGGCCCACCCCCTTGCTCATTGATTTGGGGAAT1560.3043011801423974No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.29844923437042814No Hit
TTGGACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1490.29064664000780255No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.2886959914171462No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.24773237101336196No Hit
TTGGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG1230.23992977665073636No Hit
TTGGACGGGTGATTTGAAACCTAGGTTGATTTCTTTTCTCACTCTTGAAAGGAGTATTTCTTTG1110.21652199356285967No Hit
TTGGACGGGGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA1070.20871939920023408No Hit
TTGGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.18141031893104456No Hit
TTGGACGGGACATAAGATACTAAAAGTCTCATTTTATATTTGTTTTAGATGTTTTAAATAGTTG910.1775090217497318No Hit
TTGGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.17555837315907538No Hit
TTGGACGGGGGTACTGCGTGTGACATCTCCCATGCTGCTGGTGGCTCTGGCTGTCTTCTTTGGC900.17555837315907538No Hit
TTGGACGGGGCAGCAGGAGGGGCTGGGGGCAGGTTTCATGCATGCCTTTGCTCTTCCCCTCATG890.173607724568419No Hit
TTGGACGGGTGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGGACGTTCCCTT860.16775577879644982No Hit
TTGGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG860.16775577879644982No Hit
TTGGACGGGGCTGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGTGT820.15995318443382425No Hit
TTGGACGGGGAAAGCACTGCCACCGGCACCATGCCCCACCCATACCCAGCACTGACCCCGGAGC810.15800253584316784No Hit
TTGGACGGGTGAACTCATCTTTCTGGTATGCTCCTTTGTGTCTCATTTCTGATTGCCAAAATGT770.15019994148054228No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.13849604993660392No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA710.13849604993660392No Hit
TTGGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC700.1365454013459475No Hit
TTGGACGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.13459475275529115No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC660.12874280698332194No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC650.12679215839266558No Hit
TTGGACGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12094021262069637No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12094021262069637No Hit
TTGGACGGGGAGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGC610.11898956403003998No Hit
TTGGACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.11508826684872721No Hit
TTGGACGGGAAGACAACACTAATCAGCATATCCTATATTGGATCACAGTGCTCTCCAAATTATT590.11508826684872721No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA590.11508826684872721No Hit
TTGGACGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCCTCCCACTG560.10923632107675803No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT530.10338437530478883No Hit
TTGGACGGGGTAGCTGTTCACCTTGAAGACAGGCCTTAGTGTCACGCAGGTTTATTACAGTGGC520.10143372671413244No Hit
TTGGACGGCGCCCAACCTACACCAACCTTAACCGCCTTATTAGCCAGATTGTGTCTTCCATCAC520.10143372671413244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG150.00457701258.268755
CCACTTG150.00457701258.268756
TCTGTTG150.00457701258.268754
TATCTTA150.00457701258.268758
CACTTGC150.00457701258.268757
GCGTTAG150.00457701258.268754
TCACTCA150.00457701258.268756
CTGTTGT150.00457701258.268755
CGACACT452.910383E-1158.21140744
CATTGAT150.00459489958.21140749
TATATCC150.00459489958.21140749
TCGGCGT150.00459489958.21140748
ACACTTG452.910383E-1158.21140746
CCCAAGA150.00459489958.21140746
ACGAGCG150.00459489958.21140744
CCCGACA452.910383E-1158.21140742
CACTTGG452.910383E-1158.21140747
TTGGATT452.910383E-1158.21140750
TCATTGA150.00459489958.21140748
TCTTCAT150.00459489958.21140742
Produced by FastQC (version 0.11.5)