FastQCFastQC Report
Fri 17 Jun 2016
SRR1526852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51060
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11532.2581276929103016No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.7696827262044653No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3670.7187622405013709No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2790.5464159811985899No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.49941245593419503No Hit
TTGGACGGGATGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATTTGCCCAGC2520.4935370152761457No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.47395221308264784No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.45045045045045046No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.4034469251860556No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.3995299647473561No Hit
TTGGACGGGATCTGGGTCCCGGCTGACGGATTGGCCCACCCCCTTGCTCATTGATTTGGGGAAT1710.33490011750881316No Hit
TTGGACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1710.33490011750881316No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.31727379553466506No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.23501762632197415No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.21934978456717588No Hit
TTGGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG1030.2017234625930278No Hit
TTGGACGGGGCAGCAGGAGGGGCTGGGGGCAGGTTTCATGCATGCCTTTGCTCTTCCCCTCATG960.18801410105757932No Hit
TTGGACGGGTGATTTGAAACCTAGGTTGATTTCTTTTCTCACTCTTGAAAGGAGTATTTCTTTG960.18801410105757932No Hit
TTGGACGGGGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA910.1782216999608304No Hit
TTGGACGGGACATAAGATACTAAAAGTCTCATTTTATATTTGTTTTAGATGTTTTAAATAGTTG860.16842929886408148No Hit
TTGGACGGGTGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGGACGTTCCCTT850.1664708186447317No Hit
TTGGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.1664708186447317No Hit
TTGGACGGGGCTGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGTGT840.1645123384253819No Hit
TTGGACGGGGGTACTGCGTGTGACATCTCCCATGCTGCTGGTGGCTCTGGCTGTCTTCTTTGGC800.15667841754798276No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC790.15471993732863298No Hit
TTGGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG790.15471993732863298No Hit
TTGGACGGGTGAACTCATCTTTCTGGTATGCTCCTTTGTGTCTCATTTCTGATTGCCAAAATGT790.15471993732863298No Hit
TTGGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.14884449667058364No Hit
TTGGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC700.13709361535448492No Hit
TTGGACGGGGAAAGCACTGCCACCGGCACCATGCCCCACCCATACCCAGCACTGACCCCGGAGC670.13121817469643557No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12142577359968663No Hit
TTGGACGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.11750881316098707No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11750881316098707No Hit
TTGGACGGGGGTCTTCCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGACATCGGCCGCC590.11555033294163729No Hit
TTGGACGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.11163337250293771No Hit
TTGGACGGGGGGCATAGAATGCAAAGGTGTTGGTTGCTGTTTGTTAAGTTAATTGATTGTAATC560.10967489228358794No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA560.10967489228358794No Hit
TTGGACGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCCTCCCACTG550.10771641206423815No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10771641206423815No Hit
TTGGACGGGGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCACTTGCT550.10771641206423815No Hit
TTGGACGGGAACTGACCCGGACTCACCTGGGAGGTACCTGGGCTGCCCACGGCCCATGGACATT540.10575793184488837No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC540.10575793184488837No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT540.10575793184488837No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC530.10379945162553858No Hit
TTGGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10379945162553858No Hit
TTGGACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.10184097140618881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT150.00453762958.3954444
GACACTT150.00453762958.3954445
TCCGGTA150.00453762958.3954442
TGCCCAA150.00453762958.3954444
CCCGACA150.00453762958.3954442
GCACTTG150.00453762958.3954442
TCTCCCG150.00453762958.3954439
CTTTGAC150.00453762958.3954439
CCGACAC150.00453762958.3954443
CGTCCGG150.00453762958.3954440
CACGCCT150.004555485758.33762426
TGACATC150.004555485758.33762421
GGCTGAC351.5279511E-858.33762421
ACATCGT150.004555485758.33762426
GACATCT150.004555485758.33762422
AAGATCC150.004555485758.33762421
CGGCTGA351.5279511E-858.33762420
TCTCCCA150.004555485758.33762426
TGACGGA351.5279511E-858.33762424
CATCAAT201.9738657E-458.107553
Produced by FastQC (version 0.11.5)