SRR1526851_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45345
Sequences flagged as poor quality	0
Sequence length	56
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1268	2.7963391774175763	No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.9372587936927997	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	373	0.8225824236409748	No Hit
TTGGACGGGATGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATT	246	0.5425074429374793	No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	217	0.4785533134855	No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	195	0.4300363876943433	No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	0.42342044326827655	No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.39695666556400927	No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	177	0.39034072113794244	No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	174	0.38372477671187566	No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139	0.30653875840776273	No Hit
TTGGACGGGATCTGGGTCCCGGCTGACGGATTGGCCCACCCCCTTGCTCATTGATT	138	0.30433344359907377	No Hit
TTGGACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT	106	0.23376336972102768	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	103	0.22714742529496088	No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99	0.2183261660602051	No Hit
TTGGACGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGT	97	0.2139155364428272	No Hit
TTGGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	95	0.20950490682544934	No Hit
TTGGACGGGGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGG	89	0.1962730179733157	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	85	0.18745175873855993	No Hit
TTGGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85	0.18745175873855993	No Hit
TTGGACGGGGGTGAAAGGGTGAGGTGCGTCCGCGCGAGGCGCATGCGTGCTAGGAC	85	0.18745175873855993	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	84	0.185246443929871	No Hit
TTGGACGGGGCTGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTAC	81	0.17863049950380416	No Hit
TTGGACGGGTGATTTGAAACCTAGGTTGATTTCTTTTCTCACTCTTGAAAGGAGTA	79	0.1742198698864263	No Hit
TTGGACGGGTGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGGAC	77	0.1698092402690484	No Hit
TTGGACGGGAACATAAGCTTGTCAGTGCACACATAGATGGTTGGCATGTTTAGCAA	76	0.1676039254603595	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	76	0.1676039254603595	No Hit
TTGGACGGGGTAGCTGTTCACCTTGAAGACAGGCCTTAGTGTCACGCAGGTTTATT	76	0.1676039254603595	No Hit
TTGGACGGGGGTACTGCGTGTGACATCTCCCATGCTGCTGGTGGCTCTGGCTGTCT	75	0.16539861065167052	No Hit
TTGGACGGGACATAAGATACTAAAAGTCTCATTTTATATTTGTTTTAGATGTTTTA	74	0.1631932958429816	No Hit
TTGGACGGGGAAGGATGTTGACCTTAAAGAATCCTAAGACACGCTGGCTTGGAGCT	72	0.1587826662256037	No Hit
TTGGACGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69	0.15216672179953689	No Hit
TTGGACGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCC	68	0.14996140699084795	No Hit
TTGGACGGGGCAGCAGGAGGGGCTGGGGGCAGGTTTCATGCATGCCTTTGCTCTTC	65	0.14334546256478112	No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.14114014775609218	No Hit
TTGGACGGGGAAGTAGCAGCCCACTACCCTCAGATCACCTTTGACAGCATGATTGT	64	0.14114014775609218	No Hit
TTGGACGGGAAGACAACACTAATCAGCATATCCTATATTGGATCACAGTGCTCTCC	61	0.13452420333002535	No Hit
TTGGACGGGGTCCGTTTCTCCGAGAACCGGGAAGGACGTTTGTGTAGAGGACATTG	61	0.13452420333002535	No Hit
TTGGACGGGTGAACTCATCTTTCTGGTATGCTCCTTTGTGTCTCATTTCTGATTGC	61	0.13452420333002535	No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.13452420333002535	No Hit
TTGGACGGGGAAAGCACTGCCACCGGCACCATGCCCCACCCATACCCAGCACTGAC	60	0.13231888852133644	No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	58	0.12790825890395852	No Hit
TTGGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.1257029440952696	No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.12349762928658065	No Hit
TTGGACGGGAACTGACCCGGACTCACCTGGGAGGTACCTGGGCTGCCCACGGCCCA	55	0.12129231447789172	No Hit
TTGGACGGGGCTGTTCACCTTGAAGACAGGCCTTAGTGTCACGCAGGTTTATTACA	54	0.11908699966920278	No Hit
TTGGACGGGGACGCACGCGCGAGCCACATCGTCTGTGGTCGTCCGGTATCCGAAGT	53	0.11688168486051384	No Hit
TTGGACGGGGTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTG	53	0.11688168486051384	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	51	0.11247105524313597	No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	49	0.10806042562575807	No Hit
TTGGACGGGATTTGAGAAAATGCTAATATATAGAGACAAATGGTTCTTAAAGCTTA	49	0.10806042562575807	No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	48	0.10585511081706914	No Hit
TTGGACGGGGGAAGCAGGCATCTGAGGGCCCACTGAAGGGCATCTTGGGCTACACT	48	0.10585511081706914	No Hit
TTGGACGGGGGGCATAGAATGCAAAGGTGTTGGTTGCTGTTTGTTAAGTTAATTGA	46	0.10144448119969127	No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	46	0.10144448119969127	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	20	3.796833E-4	50.884876	50
CATTGAT	20	3.818075E-4	50.827507	49
TCATTGA	20	3.8608428E-4	50.71316	48
ACACTTG	35	4.888352E-8	50.316963	46
CACTTGG	35	4.888352E-8	50.316963	47
CTCATTG	20	4.0135373E-4	50.316963	47
CGACACT	35	4.931826E-8	50.26087	44
AGAAGCC	15	0.008208038	50.26087	45
ACCGTTT	15	0.008208038	50.26087	44
CCGTTTT	15	0.008208038	50.26087	45
GACACTT	35	4.931826E-8	50.26087	45
TGCGTGC	20	4.0357368E-4	50.260868	44
GCGTGCT	20	4.0357368E-4	50.260868	45
TTACCGT	15	0.008244325	50.204903	42
CATTAAC	30	9.968244E-7	50.204903	42
TACCGTT	15	0.008244325	50.204903	43
CCCGACA	35	4.9754817E-8	50.204903	42
CCGACAC	35	4.9754817E-8	50.204903	43
CATATTC	20	4.0580338E-4	50.2049	42
GAGGCGC	20	4.0804286E-4	50.149055	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content