##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526851_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45345 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.57355827544382 39.0 38.0 40.0 36.0 40.0 2 38.53414929981255 39.0 38.0 40.0 36.0 40.0 3 38.22103870327489 39.0 38.0 40.0 35.0 40.0 4 38.30347337082369 39.0 38.0 40.0 35.0 40.0 5 37.385511081706916 39.0 37.0 40.0 33.0 40.0 6 38.45817620465321 39.0 38.0 40.0 36.0 40.0 7 38.73115007167273 39.0 39.0 40.0 38.0 40.0 8 38.67610541404785 39.0 38.0 40.0 38.0 40.0 9 38.65811004520895 39.0 38.0 40.0 37.0 40.0 10 38.05264086448341 39.0 38.0 40.0 35.0 40.0 11 37.88490462013453 39.0 38.0 40.0 35.0 40.0 12 37.797331569081486 39.0 38.0 40.0 34.0 40.0 13 37.57589590914103 39.0 38.0 40.0 33.0 40.0 14 37.590098136508985 39.0 37.0 40.0 33.0 40.0 15 37.73500937258794 39.0 38.0 40.0 33.0 40.0 16 37.6080714521998 39.0 38.0 40.0 33.0 40.0 17 37.569743080824786 39.0 38.0 40.0 33.0 40.0 18 37.47445142794134 39.0 37.0 40.0 33.0 40.0 19 37.389502701510644 39.0 37.0 40.0 33.0 40.0 20 37.2809130003308 39.0 37.0 40.0 33.0 40.0 21 37.32080714521998 39.0 37.0 40.0 33.0 40.0 22 37.20339618480538 39.0 37.0 40.0 33.0 40.0 23 37.09668100121292 39.0 36.0 40.0 33.0 40.0 24 36.86966589480648 39.0 36.0 40.0 32.0 40.0 25 36.82430256919175 39.0 36.0 40.0 32.0 40.0 26 36.49564450325284 38.0 36.0 40.0 32.0 40.0 27 36.33366413055464 38.0 36.0 40.0 31.0 40.0 28 36.137699856654535 38.0 35.0 40.0 30.0 40.0 29 36.08172896681001 38.0 35.0 40.0 30.0 40.0 30 35.775675377660164 38.0 35.0 40.0 29.0 40.0 31 35.23795346785754 38.0 35.0 39.0 28.0 40.0 32 35.20333002536112 38.0 35.0 39.0 28.0 40.0 33 34.99592016760393 38.0 35.0 39.0 27.0 40.0 34 35.10197375675378 38.0 35.0 40.0 28.0 40.0 35 34.85914654316904 38.0 34.0 40.0 27.0 40.0 36 34.02108280957107 38.0 34.0 40.0 21.0 40.0 37 33.695688609549016 38.0 33.0 40.0 18.0 40.0 38 33.491763149189545 38.0 33.0 40.0 17.0 40.0 39 33.32797441834822 38.0 33.0 40.0 15.0 40.0 40 33.100628514720476 38.0 33.0 39.0 10.0 40.0 41 32.69235858418789 38.0 33.0 39.0 4.0 40.0 42 32.46818833388466 38.0 32.0 39.0 4.0 40.0 43 32.383371926342484 38.0 32.0 39.0 4.0 40.0 44 32.16667769324071 38.0 32.0 39.0 4.0 40.0 45 31.959620685852904 37.0 32.0 39.0 4.0 40.0 46 31.480780681442276 37.0 31.0 39.0 4.0 40.0 47 31.24011467637005 37.0 31.0 39.0 4.0 40.0 48 30.925460359466314 36.0 30.0 39.0 4.0 40.0 49 30.73326717388907 36.0 30.0 39.0 4.0 40.0 50 30.41148969015327 36.0 29.0 39.0 4.0 40.0 51 29.800727753886868 36.0 29.0 39.0 4.0 40.0 52 29.595148307420885 36.0 28.0 39.0 4.0 40.0 53 29.3490351747712 35.0 27.0 39.0 4.0 40.0 54 28.870944977395524 35.0 25.0 39.0 4.0 40.0 55 28.595721689271144 35.0 23.0 39.0 4.0 40.0 56 17.331767559819163 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5009979631930008 1 2 -0.558097180959173 1 3 -0.7294021246065086 1 4 -0.49151437679248744 1 5 -1.8900776977558422 1 6 -0.6464240560461434 1 7 -0.238511191893501 1 8 -0.5456038886840702 1 9 -0.39911451388252317 1 10 -0.14165706503510478 1 11 0.0015308065569072937 1 12 0.08701349025389504 1 13 0.18967173581196306 1 14 -1.0361030111941147 1 15 -0.6947369249015907 1 16 0.05760958678676076 1 17 0.28793444209883035 1 18 -0.1751272881919732 1 19 -0.45556151641395104 1 20 0.10581576720040431 1 21 0.18355373523937146 1 22 -0.5709711135557427 1 23 -0.06228651067033297 1 24 -0.03059042773564613 1 25 -0.4412200150581356 1 26 -0.6979884527726483 1 27 -0.6599574535547603 1 28 -0.11770313282161737 1 29 -0.39311202711981963 1 30 0.5006354699270545 1 31 -0.44582182546967175 1 32 0.10147923659171454 1 33 0.22005309759248348 1 34 -0.07517053586462907 1 35 -0.30995747280002917 1 36 0.22801340880632637 1 37 0.33244301370123 1 38 1.4433074630920686 1 39 0.5201216883010744 1 40 1.4974396611940293 1 41 1.5703686533805694 1 42 1.9157604505536128 1 43 0.6615879121051265 1 44 1.8836761188464664 1 45 1.9874974798743352 1 46 0.46515543762197353 1 47 0.4991303335381865 1 48 0.7915864486521897 1 49 0.4858596531844803 1 50 0.5434927117633315 1 51 1.129910807375083 1 52 0.34335040233867176 1 53 -0.5321561327366027 1 54 0.1680731782497773 1 55 -0.6254718628671192 1 56 -0.019195377500011546 2 1 0.08558212338708415 2 2 0.10099372813173346 2 3 0.3171346719302903 2 4 0.1286154933373851 2 5 -0.23315129082943997 2 6 0.5667794071573198 2 7 0.46603426265195225 2 8 0.3569935139133307 2 9 0.47534436057635077 2 10 0.5682996449216091 2 11 0.34893340395950645 2 12 -0.0893934361530313 2 13 -0.02786073172050152 2 14 -0.8120770371681374 2 15 -0.1720096521743173 2 16 -0.2692302400530622 2 17 -0.975052570888181 2 18 -0.21192382498851003 2 19 -0.5670333778858065 2 20 -0.5002448388602048 2 21 -0.3554073037216625 2 22 -0.23655552914016198 2 23 0.07299487461104803 2 24 0.13824074109552242 2 25 -0.2518260756641979 2 26 -0.14495814974235088 2 27 0.266449472852166 2 28 0.21995920484071974 2 29 -0.19830683231462842 2 30 0.23764845694004322 2 31 0.3333989537511073 2 32 -0.18748180236931944 2 33 0.1854210629604509 2 34 0.5092450485509517 2 35 0.6619039990614439 2 36 0.7410004217933377 2 37 0.4688066500648631 2 38 0.9238269436115445 2 39 0.6618965800759682 2 40 1.0829374966918621 2 41 2.1309747139866317 2 42 0.8551543899475504 2 43 2.001414751931968 2 44 1.9929834781538247 2 45 2.3089260513029046 2 46 2.7822550047215415 2 47 3.055407389815244 2 48 2.4842271412928802 2 49 3.2672449345697636 2 50 3.520765439036058 2 51 4.0238502013144775 2 52 4.257852566840839 2 53 3.278449927869456 2 54 4.471103481280078 2 55 4.955696968301712 2 56 2.882319774015137 3 1 -0.28373605843109573 3 2 -0.34976384762583734 3 3 -0.49725926746364735 3 4 -0.43199056726867724 3 5 -1.1043634120415575 3 6 -0.08094786556995359 3 7 0.05315547477316329 3 8 0.08237230179211963 3 9 -0.03601927578728237 3 10 0.20655722067917992 3 11 -0.1681120505859468 3 12 0.6435611093015154 3 13 0.9664574500976784 3 14 0.03830175071065156 3 15 0.43026307509840933 3 16 0.32546672964390666 3 17 0.27305348971788135 3 18 0.036182235617552294 3 19 0.46705753120509996 3 20 0.2516491005337329 3 21 -0.34025578857014693 3 22 0.2445050769204471 3 23 0.7680706321868058 3 24 -0.10797138011659513 3 25 0.037946651608528725 3 26 0.3228448805606803 3 27 0.5126615940642836 3 28 0.9775349624164775 3 29 0.6426022585944651 3 30 0.7357545175461055 3 31 1.3220353173874742 3 32 0.8693363794488604 3 33 2.0206483356877243 3 34 1.4278056546115607 3 35 1.6751615748190147 3 36 0.8053943611872754 3 37 0.7282763470345657 3 38 1.2439027011873023 3 39 0.16000264068203052 3 40 -0.6841079578535911 3 41 -0.4087980132860949 3 42 -0.25090621611305863 3 43 -1.0288882783710669 3 44 -0.7740619763916285 3 45 -0.2535739486970954 3 46 -1.371154086187552 3 47 -1.0961077616999084 3 48 -0.9286516465859052 3 49 -0.906997489672662 3 50 -0.26900728823666853 3 51 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0.5529702387451181 5 21 0.777630390291641 5 22 -0.7260233783641112 5 23 0.987074697227456 5 24 -0.1821583719865174 5 25 -0.31113871424513206 5 26 0.5662391895037686 5 27 0.9405071225195698 5 28 -0.26230243595750125 5 29 0.6243095756676382 5 30 1.1905309403103317 5 31 0.8921572686069865 5 32 1.3540924770098357 5 33 1.8605873600779717 5 34 2.050265004205052 5 35 2.168555883762103 5 36 1.8429959872035369 5 37 1.3812235015061063 5 38 1.514736034520638 5 39 1.517218087836497 5 40 2.63448960312202 5 41 2.6232141818358556 5 42 3.3385246781958884 5 43 2.3938759492712123 5 44 3.3738038772669086 5 45 3.66868214886388 5 46 2.4901264016173243 5 47 2.517713376511473 5 48 3.1028524184547415 5 49 3.298287063172868 5 50 4.10446832151943 5 51 4.833162839895412 5 52 4.803861436020441 5 53 5.362152810352828 5 54 4.895715454672544 5 55 4.196827788700826 5 56 2.54584527290649 6 1 -0.23038239989450915 6 2 -0.04590205900795041 6 3 -0.4251047959189336 6 4 0.01719642460124504 6 5 -0.41330650147245507 6 6 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2.148662986983183 6 47 1.8591767911456216 6 48 2.3467548574791373 6 49 2.4934090143923804 6 50 1.616663443470646 6 51 2.9795043033100406 6 52 3.4867882652887303 6 53 3.898738176206482 6 54 4.164008137599371 6 55 2.1236570569935083 6 56 0.6434062485162464 7 1 -0.5962360584310957 7 2 -0.9727313273006359 7 3 -0.4251047959189336 7 4 -0.7389011363743663 7 5 -0.5352577209846459 7 6 -0.2608259143504412 7 7 -0.6897307040886247 7 8 -0.5665098120290182 7 9 -0.4171168367628937 7 10 -0.12017042159723701 7 11 -0.5141486359517984 7 12 -0.7939388906984846 7 13 -0.9131157206340319 7 14 -0.6563120704275605 7 15 -0.7679076566089051 7 16 -0.8035983110065033 7 17 -0.4002188680057017 7 18 0.07632451204031554 7 19 -0.23721076147782583 7 20 -0.739712688084154 7 21 -0.5150525365376311 7 22 -0.2869989881202031 7 23 0.18219664844696837 7 24 0.5251587011842176 7 25 -0.38430944595244654 7 26 0.6394099212110902 7 27 -0.15705385309018283 7 28 0.1767219542863998 7 29 -0.8878855462835844 7 30 -0.4680056450555199 7 31 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0.009990539167716861 8 17 -0.8073036531392646 8 18 0.2058250927604064 8 19 -0.14603770689014084 8 20 0.7486729100575431 8 21 0.040696592382232666 8 22 0.4290288864442573 8 23 0.05676110837728743 8 24 0.6360762389310182 8 25 0.22544665160852873 8 26 -0.15036940515360442 8 27 0.6019473083499989 8 28 1.2632492481307622 8 29 1.0116498776420855 8 30 1.3339688032603902 8 31 2.2208448411969997 8 32 1.5538601889726706 8 33 0.6248150023543886 8 34 1.3534008927067944 8 35 2.3328996700571096 8 36 1.513727694520611 8 37 2.189585870844084 8 38 2.9909265107111125 8 39 2.805835974015359 8 40 3.1641063278606936 8 41 3.5227496057615255 8 42 2.939569974363131 8 43 2.8044450549622653 8 44 3.0027237378940868 8 45 3.9398784322552842 8 46 3.2270601995267327 8 47 3.0705589049667594 8 48 4.0058721629379015 8 49 3.5810977484225752 8 50 2.972064140334762 8 51 3.963244140708415 8 52 4.771921830910102 8 53 4.634510533930065 8 54 4.9537874639640656 8 55 4.588813851418596 8 56 4.814137955833321 9 1 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-0.654247983319955 120 29 -0.20042099721948858 120 30 0.14515374235230638 120 31 0.30168648017816935 120 32 -0.744035713574398 120 33 0.9348925217342341 120 34 1.200576972441013 120 35 1.4663437453616552 120 36 1.7811695549857305 120 37 1.4254662695151836 120 38 1.677526732195055 120 39 -0.1515283670699077 120 40 0.4365315770301308 120 41 -0.5730421993326047 120 42 0.6754503730342307 120 43 0.7308016441095546 120 44 0.6721589538409276 120 45 0.8961353536284875 120 46 0.2785552161380309 120 47 0.22559766465667863 120 48 -0.10985319697350349 120 49 0.9902893320327628 120 50 -0.06115845102736728 120 51 0.5756472414836118 120 52 0.6484445330141995 120 53 0.1500144098990539 120 54 0.9161351937536537 120 55 0.3579168414518179 120 56 -0.46493181160854036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 9.0 5 6.0 6 7.0 7 10.0 8 16.0 9 16.0 10 19.0 11 34.0 12 41.0 13 38.0 14 75.0 15 97.0 16 156.0 17 166.0 18 214.0 19 257.0 20 286.0 21 394.0 22 459.0 23 474.0 24 731.0 25 1155.0 26 784.0 27 738.0 28 828.0 29 805.0 30 968.0 31 1093.0 32 1388.0 33 1751.0 34 2213.0 35 2954.0 36 4141.0 37 6511.0 38 8607.0 39 7904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.27004079832396 0.5336861837027236 0.15657735141691476 0.03969566655640092 8 99.44401544401545 0.4214009928295643 0.0882515168229454 0.04633204633204633 9 97.92920939464108 1.0475245341272466 0.3263865916859632 0.6968794795457052 10 55.856213474473485 25.526518910574485 10.424523100672621 8.192744514279413 11 36.594993935384274 32.482081817179406 16.169368177307312 14.753556070129012 12 36.08997684419451 20.218326166060205 18.862057558716508 24.82963943102878 13 28.94696217885103 19.783879148748483 25.257470503914437 26.011688168486053 14 22.93747932517367 24.76127467195942 31.377219098026245 20.924026904840666 15 29.07707575256368 24.721579005403022 27.045980813761165 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28.044850571668213 23.539928486293206 29 24.851443592745586 27.07150588703086 28.31297355806402 19.764076962159535 30 22.438150825761095 24.25548849240565 32.75187371493003 20.55448696690323 31 21.473912370335967 26.523345055625597 33.13869904673435 18.864043527304094 32 26.073877460738775 22.234019022340192 33.74917053749171 17.94293297942933 33 24.230624571340073 21.717294630412177 32.772848956835325 21.27923184141242 34 20.939442883377215 25.492842445295043 39.026926565922516 14.540788105405225 35 18.17638464603533 26.397928011688137 40.339575862221636 15.086111480054898 36 21.259964570416297 28.03144375553587 34.26262178919398 16.445969884853852 37 20.492493024491782 26.575579077904248 35.91390229859604 17.018025599007927 38 25.489631336405534 24.291031549096065 32.81637717121588 17.40295994328252 39 20.477520617185423 22.144630664183737 35.10907156158552 22.268777157045314 40 22.134220743205766 21.974486966167497 36.18191902384914 19.709373266777593 41 20.18153170147134 20.973791083198332 38.47451232773352 20.370164887596815 42 20.84128745837958 21.44062153163152 36.872364039955606 20.845726970033297 43 18.393356720991164 20.302855366579333 42.08668235712065 19.21710555530885 44 20.444553004396678 20.253586179331172 36.15712572722832 23.144735089043834 45 19.470913573665623 20.892472401768064 37.93340885364608 21.703205170920235 46 17.269192311965337 22.3975113876236 34.267303632929675 26.065992667481392 47 18.199195394634483 25.63846102553844 35.9116267697984 20.25071681002867 48 18.24046268490713 27.505283060838615 35.64230897564231 18.611945278611948 49 21.422060164083863 25.092825221780018 35.1935433666096 18.291571247526512 50 21.399167797779313 24.031507977125564 35.44647426626021 19.122849958834916 51 18.122581506026776 28.041186674376195 36.31410398968109 17.522127829915938 52 18.730949785302688 25.70805615502703 38.36296082052195 17.19803323914833 53 21.799007720203793 22.724542238636616 37.223841413220015 18.252608627939573 54 19.092538651131527 26.190902980058254 35.30808872955411 19.408469639256108 55 20.52720634072491 25.955116216938283 34.330750734704786 19.186926707632026 56 20.870242599599376 23.058090362786558 34.3690184731805 21.702648564433563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 2.0 7 1.0 8 8.5 9 16.0 10 140.0 11 264.0 12 292.5 13 343.0 14 365.0 15 341.0 16 317.0 17 560.0 18 803.0 19 900.0 20 997.0 21 912.0 22 806.0 23 785.0 24 858.5 25 932.0 26 1056.0 27 1180.0 28 1251.0 29 1356.5 30 1391.0 31 1452.0 32 1513.0 33 1193.0 34 873.0 35 900.0 36 927.0 37 806.5 38 732.5 39 779.0 40 781.0 41 783.0 42 876.5 43 970.0 44 1366.5 45 1763.0 46 2216.5 47 1928.5 48 1187.0 49 1285.5 50 1384.0 51 1473.5 52 1563.0 53 1573.5 54 1763.0 55 1942.0 56 2058.0 57 2174.0 58 2299.0 59 2424.0 60 3015.0 61 3606.0 62 3151.5 63 2299.5 64 1902.0 65 1654.0 66 1406.0 67 1276.5 68 1147.0 69 876.5 70 606.0 71 496.5 72 362.0 73 337.0 74 267.5 75 198.0 76 162.0 77 126.0 78 107.0 79 69.0 80 50.0 81 39.5 82 29.0 83 20.0 84 11.0 85 8.5 86 6.0 87 4.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.04410629617377881 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.004410629617377881 21 0.0 22 0.0 23 0.0 24 0.004410629617377881 25 0.013231888852133642 26 0.06836475906935716 27 0.06174881464329032 28 0.08600727753886868 29 0.1676039254603595 30 0.2514058881905392 31 0.2911015547469401 32 0.29771749917300694 33 0.3219759620685853 34 0.3263865916859632 35 0.3771088322858088 36 0.407983239607454 37 0.41239386922483184 38 0.46091079501598853 39 0.5226596096592788 40 0.5954349983460139 41 0.626309405667659 42 0.6505678685632373 43 0.6770316462675047 44 0.6858529055022605 45 0.7145219980152167 46 0.7498070349542397 47 0.7806814422758849 48 0.8600727753886867 49 0.8115558495975301 50 0.8909471827103319 51 0.8358143124931084 52 0.8777152938581982 53 0.8777152938581982 54 1.5768000882125925 55 0.9460800529275554 56 0.9152056456059102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 45345.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.95468078068144 #Duplication Level Percentage of deduplicated Percentage of total 1 66.47984601449275 25.897011798434228 2 12.771739130434783 9.950380416804498 3 6.66893115942029 7.793582533906715 4 3.940217391304348 6.1395964273900105 5 2.4513134057971016 4.774506560811556 6 1.8795289855072463 4.392987098908369 7 1.0416666666666665 2.8404454735913554 8 0.8944746376811594 2.7875179181828207 9 0.6906702898550725 2.4214356599404563 >10 2.9042119565217392 18.868673503142574 >50 0.198143115942029 5.460359466313816 >100 0.07359601449275363 5.877163965156026 >500 0.0 0.0 >1k 0.005661231884057971 2.7963391774175763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1268 2.7963391774175763 No Hit TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.9372587936927997 No Hit TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 373 0.8225824236409748 No Hit TTGGACGGGATGTAAAGATGTTGTACAGAACTGTTTCCTCTCCCGACACTTGGATT 246 0.5425074429374793 No Hit TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.4785533134855 No Hit TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 195 0.4300363876943433 No Hit TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 192 0.42342044326827655 No Hit TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 180 0.39695666556400927 No Hit TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.39034072113794244 No Hit TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 174 0.38372477671187566 No Hit TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.30653875840776273 No Hit TTGGACGGGATCTGGGTCCCGGCTGACGGATTGGCCCACCCCCTTGCTCATTGATT 138 0.30433344359907377 No Hit TTGGACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT 106 0.23376336972102768 No Hit TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 103 0.22714742529496088 No Hit TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.2183261660602051 No Hit TTGGACGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGT 97 0.2139155364428272 No Hit TTGGACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA 95 0.20950490682544934 No Hit TTGGACGGGGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGG 89 0.1962730179733157 No Hit TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 85 0.18745175873855993 No Hit TTGGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.18745175873855993 No Hit TTGGACGGGGGTGAAAGGGTGAGGTGCGTCCGCGCGAGGCGCATGCGTGCTAGGAC 85 0.18745175873855993 No Hit TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 84 0.185246443929871 No Hit TTGGACGGGGCTGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTAC 81 0.17863049950380416 No Hit TTGGACGGGTGATTTGAAACCTAGGTTGATTTCTTTTCTCACTCTTGAAAGGAGTA 79 0.1742198698864263 No Hit TTGGACGGGTGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGGAC 77 0.1698092402690484 No Hit TTGGACGGGAACATAAGCTTGTCAGTGCACACATAGATGGTTGGCATGTTTAGCAA 76 0.1676039254603595 No Hit TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 76 0.1676039254603595 No Hit TTGGACGGGGTAGCTGTTCACCTTGAAGACAGGCCTTAGTGTCACGCAGGTTTATT 76 0.1676039254603595 No Hit TTGGACGGGGGTACTGCGTGTGACATCTCCCATGCTGCTGGTGGCTCTGGCTGTCT 75 0.16539861065167052 No Hit TTGGACGGGACATAAGATACTAAAAGTCTCATTTTATATTTGTTTTAGATGTTTTA 74 0.1631932958429816 No Hit TTGGACGGGGAAGGATGTTGACCTTAAAGAATCCTAAGACACGCTGGCTTGGAGCT 72 0.1587826662256037 No Hit TTGGACGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.15216672179953689 No Hit TTGGACGGGGTGGACTGGTGCCCCACTGGCTTCAAGGTTGGCATTAACTACCAGCC 68 0.14996140699084795 No Hit TTGGACGGGGCAGCAGGAGGGGCTGGGGGCAGGTTTCATGCATGCCTTTGCTCTTC 65 0.14334546256478112 No Hit TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.14114014775609218 No Hit TTGGACGGGGAAGTAGCAGCCCACTACCCTCAGATCACCTTTGACAGCATGATTGT 64 0.14114014775609218 No Hit TTGGACGGGAAGACAACACTAATCAGCATATCCTATATTGGATCACAGTGCTCTCC 61 0.13452420333002535 No Hit TTGGACGGGGTCCGTTTCTCCGAGAACCGGGAAGGACGTTTGTGTAGAGGACATTG 61 0.13452420333002535 No Hit TTGGACGGGTGAACTCATCTTTCTGGTATGCTCCTTTGTGTCTCATTTCTGATTGC 61 0.13452420333002535 No Hit TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.13452420333002535 No Hit TTGGACGGGGAAAGCACTGCCACCGGCACCATGCCCCACCCATACCCAGCACTGAC 60 0.13231888852133644 No Hit TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 58 0.12790825890395852 No Hit TTGGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.1257029440952696 No Hit TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.12349762928658065 No Hit TTGGACGGGAACTGACCCGGACTCACCTGGGAGGTACCTGGGCTGCCCACGGCCCA 55 0.12129231447789172 No Hit TTGGACGGGGCTGTTCACCTTGAAGACAGGCCTTAGTGTCACGCAGGTTTATTACA 54 0.11908699966920278 No Hit TTGGACGGGGACGCACGCGCGAGCCACATCGTCTGTGGTCGTCCGGTATCCGAAGT 53 0.11688168486051384 No Hit TTGGACGGGGTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTG 53 0.11688168486051384 No Hit TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 51 0.11247105524313597 No Hit TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 49 0.10806042562575807 No Hit TTGGACGGGATTTGAGAAAATGCTAATATATAGAGACAAATGGTTCTTAAAGCTTA 49 0.10806042562575807 No Hit TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 48 0.10585511081706914 No Hit TTGGACGGGGGAAGCAGGCATCTGAGGGCCCACTGAAGGGCATCTTGGGCTACACT 48 0.10585511081706914 No Hit TTGGACGGGGGGCATAGAATGCAAAGGTGTTGGTTGCTGTTTGTTAAGTTAATTGA 46 0.10144448119969127 No Hit TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 46 0.10144448119969127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0022053148086889404 0.0 0.0 0.0 0.0 11 0.013231888852133642 0.0 0.0 0.0 0.0 12 0.017642518469511523 0.0 0.0 0.0 0.0 13 0.01984783327820046 0.0 0.0 0.0 0.0 14 0.022053148086889404 0.0 0.0 0.0 0.0 15 0.033079722130334104 0.0 0.0 0.0 0.0 16 0.03749035174771199 0.0 0.0 0.0 0.0 17 0.041900981365089865 0.0 0.0 0.0 0.0 18 0.04410629617377881 0.0 0.0 0.0 0.0 19 0.04631161098246775 0.0 0.0 0.0 0.0 20 0.050722240599845626 0.0 0.0 0.0 0.0 21 0.05513287021722351 0.0 0.0 0.0 0.0 22 0.06174881464329033 0.0 0.0 0.0 0.0 23 0.06615944426066821 0.0 0.0 0.0 0.0 24 0.06836475906935716 0.0 0.0 0.0 0.0 25 0.07498070349542398 0.0 0.0 0.0 0.0 26 0.0926232219649355 0.0 0.0 0.0 0.0 27 0.09482853677362443 0.0 0.0 0.0 0.0 28 0.11247105524313596 0.0 0.0 0.0 0.0 29 0.12129231447789172 0.0 0.0 0.0 0.0 30 0.12349762928658066 0.0 0.0 0.0 0.0 31 0.13011357371264748 0.0 0.0 0.0 0.0 32 0.13231888852133641 0.0 0.0 0.0 0.0 33 0.1367295181387143 0.0 0.0 0.0 0.0 34 0.14555077737347005 0.0 0.0 0.0 0.0 35 0.15657735141691476 0.0 0.0 0.0 0.0 36 0.15657735141691476 0.0 0.0 0.0 0.0 37 0.1587826662256037 0.0 0.0 0.0 0.0 38 0.1631932958429816 0.0 0.0 0.0 0.0 39 0.16539861065167052 0.0 0.0 0.0 0.0 40 0.16539861065167052 0.0 0.0 0.0 0.0 41 0.1698092402690484 0.0 0.0 0.0 0.0 42 0.1742198698864263 0.0 0.0 0.0 0.0 43 0.1742198698864263 0.0 0.0 0.0 0.0 44 0.17642518469511523 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGGAC 20 3.796833E-4 50.884876 50 CATTGAT 20 3.818075E-4 50.827507 49 TCATTGA 20 3.8608428E-4 50.71316 48 ACACTTG 35 4.888352E-8 50.316963 46 CACTTGG 35 4.888352E-8 50.316963 47 CTCATTG 20 4.0135373E-4 50.316963 47 CGACACT 35 4.931826E-8 50.26087 44 AGAAGCC 15 0.008208038 50.26087 45 ACCGTTT 15 0.008208038 50.26087 44 CCGTTTT 15 0.008208038 50.26087 45 GACACTT 35 4.931826E-8 50.26087 45 TGCGTGC 20 4.0357368E-4 50.260868 44 GCGTGCT 20 4.0357368E-4 50.260868 45 TTACCGT 15 0.008244325 50.204903 42 CATTAAC 30 9.968244E-7 50.204903 42 TACCGTT 15 0.008244325 50.204903 43 CCCGACA 35 4.9754817E-8 50.204903 42 CCGACAC 35 4.9754817E-8 50.204903 43 CATATTC 20 4.0580338E-4 50.2049 42 GAGGCGC 20 4.0804286E-4 50.149055 36 >>END_MODULE