FastQCFastQC Report
Fri 17 Jun 2016
SRR1526850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125809
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.6533713804258836No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.3147628548037104No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGTGGTATCAA3160.2511743992878093No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.2225595943056538No Hit
TCACTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.13433061227734105No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1530.12161292117416082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTTA258.556386E-658.077916
TTGGTAC201.9901773E-458.0779045
CCAGGTA150.004660073758.05471452
CAGTTGC150.004660073758.05471448
GATTTGC150.004660073758.05471437
TTATCCT150.004660073758.05471444
TCTACGT258.576686E-658.05471438
CTACGTA258.576686E-658.05471439
TAGGGCA150.004660073758.05471442
CAATGAG150.004660073758.05471446
TAGGCTC258.576686E-658.05471441
ACGTACT258.576686E-658.05471441
TGAATGG150.004660073758.05471433
AGAATGC150.004660073758.05471453
GCCCGTT150.004660073758.05471454
TACAATA150.004660073758.05471455
TACGTAC258.576686E-658.05471440
GGATAGC150.004674834758.0083831
TCTGCAA150.004689630657.96212429
CGGAATG202.0099207E-457.96212430
Produced by FastQC (version 0.11.5)