FastQCFastQC Report
Fri 17 Jun 2016
SRR1526849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161753
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.7721649675740172No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG8030.4964359239086755No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.33136943364265264No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5260.3251871680896181No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3940.24358126278956185No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3850.23801722379183077No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3600.22256155990924434No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3540.2188522005774236No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.17928570103800237No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2840.17557634170618167No Hit
TCACTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2440.1508472794940434No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC2260.13971920149858116Illumina Single End Adapter 2 (96% over 31bp)
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2260.13971920149858116No Hit
TCACTTGGGAGCATCGTGGATGTCATCTTTTTGGTGGACATTGTTTTGAATTTTCACACCACCT2010.12426353761599476No Hit
TCACTTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1980.1224088579500844No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1970.12179063139478093No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1920.11869949861826365No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1890.11684481895235328No Hit
TCACTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG1760.10880787373340835No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1740.10757142062280142No Hit
TCACTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.10757142062280142No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1730.10695319406749798No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1710.10571674095689106No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1700.1050985144015876No Hit
TCACTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1660.10262560818037378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAA150.00462091258.186253
AAATCAA201.973637E-458.186257
TTCTAAC258.473393E-658.18619557
CTGCATT150.00462660458.16811854
TTATTCA201.9766731E-458.16811854
GATTTAC201.9827567E-458.13199251
ACGAAGC258.5203665E-658.13198551
CCCACGA201.985804E-458.11394548
GACGAAG150.004643710358.1139450
TGATATA201.9888551E-458.0959143
CATAAGG201.9888551E-458.0959140
AACTTAT150.00464942358.09590545
CCTACTT150.00464942358.09590545
CCTAATT150.00464942358.09590542
AGACGCA150.00464942358.09590544
CCACTAA150.00464942358.09590542
ATAAGCC150.00464942358.09590539
TACAAAT150.00464942358.09590543
TCACAAC351.6083504E-858.095946
ATATAGG258.551802E-658.095945
Produced by FastQC (version 0.11.5)