FastQCFastQC Report
Fri 17 Jun 2016
SRR1526848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161096
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.7231712767542334No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG7840.4866663356011322No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.29733823310324276No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4600.28554402343943985No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3920.2433331678005661No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3360.20857128668619954No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3310.20546754730098823No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3220.1998808164076079No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3180.19739782489943883No Hit
TCACTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2220.13780602870338182No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2210.13718528082633957No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.13656453294929732No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC2140.13284004568704375Illumina Single End Adapter 2 (96% over 31bp)
TCACTTGGGAGCATCGTGGATGTCATCTTTTTGGTGGACATTGTTTTGAATTTTCACACCACCT2140.13284004568704375No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1880.11670060088394497No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1740.10801013060535332No Hit
TCACTTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1690.10490639122014203No Hit
TCACTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1620.10056115608084619No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1620.10056115608084619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG150.004507302358.55282245
TTAAGGG150.004507302358.55282245
TGATATA150.004524216558.4974843
CAACCGC150.004524216558.4974843
TATTGCC150.004524216558.4974842
ACCAGTT150.004524216558.4974841
CTTACAA258.209132E-658.4974843
GCAAATC150.00452986558.47905739
CAACTTG150.00455251258.40547626
GATCATA258.286135E-658.4054726
ATTTAGT150.004558186558.38710825
GTTGAGC201.940227E-458.38710825
ACGCGAT150.00456955258.3504124
CAAAGTA150.004575242858.33207723
ACTAGCA150.004580938758.3137622
TTCTACA258.363739E-658.31375521
ACAGTTT150.004638188558.13118455
ATTAGGC150.004638188558.13118454
ACCGCTA150.004638188558.13118458
GACGTAG201.9828438E-458.13118456
Produced by FastQC (version 0.11.5)