FastQCFastQC Report
Fri 17 Jun 2016
SRR1526847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146177
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12340.8441820532641935No Hit
TCACTTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6690.45766433843901566No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.3919905320262421No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4920.33657825786546447No Hit
TCACTTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3560.24354036544736857No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3020.2065988493401835No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2780.19018039773699008No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2600.17786655903459506No Hit
TCACTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2500.17102553753326447No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.1689732310828653No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2310.15802759668073638No Hit
TCACTTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2090.1429773493778091No Hit
TCACTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2020.13818863432687767No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGT1960.13408402142607934No Hit
TCACTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1920.1313476128255471No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1900.129979408525281No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1850.1265588977746157No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1820.12450659132421653No Hit
TCACTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1600.10945634402128926No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1590.10877224187115621No Hit
TCACTTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1580.10808813972102314No Hit
TCACTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1550.10603583327062396No Hit
TCACTTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC1500.10261532251995868No Hit
TCACTTGGGAGCATCGTGGATGTCATCTTTTTGGTGGACATTGTTTTGAATTTTCA1490.10193122036982563No Hit
TCACTTGGGGGATCCTGACCTTCTACACAGCCATCTTGGTCCCTTACAACGTCTCC1470.1005630160695595No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG1470.1005630160695595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG150.00795029150.72543750
TTCATCG150.00795029150.72543750
TCTTGAT150.00796125250.70776748
TCACCCG150.00796125250.70776748
ACACAGT150.00796125250.70776748
ATTGTGG150.00796125250.70776749
AGTTACT150.00823895450.27002745
ATGCCCG204.0622987E-450.27002746
CTGCAAT150.008250209550.25267444
TCATATA150.008250209550.25267444
CCAGTAT150.00826147750.23533242
TCACATG150.00829534750.18338441
TGACGTG150.00829534750.18338441
CTCTCTA150.00829534750.18338441
CGAGTGA204.1672442E-450.01099430
CGTTAAA204.1672442E-450.01099431
GCGGGTA301.0424137E-649.99381629
TTCGGTA150.00845477749.94236825
ACATTAA252.0926564E-549.94236425
ACGCGAT252.1011698E-549.90812324
Produced by FastQC (version 0.11.5)