FastQCFastQC Report
Fri 17 Jun 2016
SRR1526846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59417
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1110.18681522123298047No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.17671710116633285No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.14810576097749803No Hit
ACGAGCGGGATCATATTCGTAGGAGTAATCATAACATTCTTCCCTCAACATTTCCTGGGCCTTT780.1312755608664187No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC770.12959254085531077No Hit
ACGAGCGGGATCACCTGCTGAGAAGAAAGTGCATTCTGTAGTGTTTGAGCAGAATGTTCTGTAG720.12117744079977111No Hit
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG700.11781140077755524No Hit
ACGAGCGGGCAGCTTTCCTTTACACACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGA680.11444536075533938No Hit
ACGAGCGGGATGTTTTAATTTATGTTTTTAAAGCTATGGAAGGGGAGAATGAAGTGGTGATATT640.10771328071090766No Hit
ACGAGCGGGGATGAATCTCAGTCCTTCAGCCATGAAGAACCTCCTGCATCACATTCTCAGTGGC620.10434724068869179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAT150.00447227158.62169357
ACTTGAC150.00462559358.1253250
GCTCATT150.00462559358.1253250
AATGAGG150.004641137658.07614549
TAAGACT150.004641137658.07614546
TAGAGGG150.004656721458.0270534
GCCTTCA150.004656721458.0270534
GATAAGA201.990159E-458.0270533
ACTTGAA201.990159E-458.0270545
TAGACAA150.004656721458.0270544
CACATGT150.004656721458.0270539
ATGTGGA150.004656721458.0270542
GCTATCT150.004656721458.0270532
ATTGTGC201.990159E-458.0270529
AAGGTGC150.004656721458.0270530
GGATAAG201.990159E-458.0270532
GAGACTA150.004656721458.0270535
ATTTTAC150.004656721458.0270532
GTAGACA150.004656721458.0270543
CAAGGTG150.004656721458.0270529
Produced by FastQC (version 0.11.5)