FastQCFastQC Report
Fri 17 Jun 2016
SRR1526845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89622
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4520.504340452121131No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3400.3793711365512932No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2530.28229675749257993No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2530.28229675749257993No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2510.28006516257168995No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2480.27671777019035504No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2310.2577492133627904No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1950.2175805047867711No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.21311731494499117No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1700.1896855682756466No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1560.17406440382941687No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1510.16848541652719198No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC1470.16402222668541205No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1410.15732744192274217No Hit
ACGAGCGGGTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1310.1461694673182924No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1270.14170627747651246No Hit
ACGAGCGGGCAGCTTTCCTTTACACACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGA1210.13501149271384258No Hit
ACGAGCGGGATTGGAGTTTTGGTTCTCTCCTGGTATCCACCTGGCATGGCTGATGATAATGGAG1200.13389569525339762No Hit
ACGAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1190.13277989779295263No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1190.13277989779295263No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1130.12608511303028275No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1130.12608511303028275No Hit
ACGAGCGGGGATGAATCTCAGTCCTTCAGCCATGAAGAACCTCCTGCATCACATTCTCAGTGGC1090.12162192318850283No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1080.12050612572805784No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1050.1171587333467229No Hit
ACGAGCGGGATCACCTGCTGAGAAGAAAGTGCATTCTGTAGTGTTTGAGCAGAATGTTCTGTAG1020.11381134096538796No Hit
ACGAGCGGGGCTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1020.11381134096538796No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1000.11157974604449801No Hit
ACGAGCGGGGGAGCTACCCAGCTTCCTGTGGTACCAAGGAGTTATTTTGGATGATTAGAAGCAC980.10934815112360805No Hit
ACGAGCGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG980.10934815112360805No Hit
ACGAGCGGGCGCCTGAGTCTTACATCACCAGGACAAGGCTTTGCCAACTTCTCATGGGTCTGAG940.10488496128182813No Hit
ACGAGCGGGCGGCCAAGAGTCCAATGAAGAGGCTATCTCCGAAGACTTGAAGGAAGGGGCCACT930.10376916382138315No Hit
ACGAGCGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG920.10265336636093816No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.10153756890049319No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC900.10042177144004821No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT900.10042177144004821No Hit
ACGAGCGGGATGTTTTAATTTATGTTTTTAAAGCTATGGAAGGGGAGAATGAAGTGGTGATATT900.10042177144004821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATA201.983398E-458.09966756
AGACCTG258.517463E-658.09966753
GCTAAGG201.983398E-458.09966754
AAGGCCG201.983398E-458.09966757
AAATAGA150.00464146158.09966358
CTATCGG150.00464146158.09966354
CCACTAG150.00464146158.09966358
ATGAGAT150.00464146158.09966352
GGATTAA150.00464146158.09966356
CCTATCG150.00464146158.09966353
AAAATAG150.00464146158.09966357
TGACTTG150.00464146158.09966354
TTAGATG150.004651766758.0671548
AGAAAGA150.004651766758.0671549
CGTCCGT150.004651766758.0671550
ATAATTG150.00466208958.0346730
TCTAGCG150.00466208958.0346736
CGTTATC150.00466208958.0346742
AATTGAT150.00466208958.0346732
ATTGATG150.00466208958.0346733
Produced by FastQC (version 0.11.5)